| Project/Area Number |
20591327
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Keio University |
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Principal Investigator |
MASUNAGA Takuji Keio University, 医学部, 講師 (70424140)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKO Akira 慶應義塾大学, 医学部, 講師 (10202988)
|
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Yoshifumi 北里大学, 北里研究所病院・皮膚科, 部長 (10306772)
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| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 遺伝子 / 病理学 / 基底膜 / ラミニン332 / 表皮水疱症 / 免疫電顕 / 表皮水庖症 |
|---|
| Research Abstract |
The epidermal basement membrane, an important structure to adhere the epidermis to the dermis, is consisted of many molecules. To clarify the in vivo interaction between these components gives a clue to elucidating the pathophysiology of skin bullous diseases showing blister formation in the epidermal basement membrane zone. In the present study, we employed post-embedding immunoelectron microscopy using human skin lacking laminin 332 (former laminin 5) to clarify the ultrastructural distribution of basement membrane components, including type XVII collagen. As a result, ultrastructural distribution of C-terminus of type XVII collagen in human skin lacking laminin 332 was different from that in normal human skin, indicating in vivo interaction between laminin 332 and type XVII collagen.
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