Establishment of remodeling technologies for protein misfolding in keratin disorders using an inducible transgenic mouse model

• Project/Area Number: 20591334
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Akita University
• Principal Investigator: MANABE Motomu Akita University, 大学院・医学系研究科, 教授 (30138309)
• Co-Investigator(Renkei-kenkyūsha): 河村 七美 秋田大学, 医学部, 助教 (70323152)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ケラチン / 分子シャペロン / 蛋白フォールディング / 細胞死

Research Abstract

In this grant program, we attempted to elucidate the molecular mechanisms of protein misfolding in keratin disorders. The transgenic mouse studies showed that mutated keratins cause neither collapse of keratins or apoptosis in cultured keratinocytes. These findings may be because of the DNA break of transgene in a certain point.