Comprehensive analysis of human hemidesmosomal proteins by mass spectrometry

• Project/Area Number: 20591341
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Nagoya University
• Principal Investigator: HIRAKO Yoshiaki Nagoya University, 理学研究科, 講師 (50377909)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞接着 / 表皮水疱症 / 自己免疫疾患 / ヘミデスモソーム / 質量分析 / 蛋白質 / 細胞・組織 / 生体分子

Research Abstract

Hemidesmosomes (HDs) are protein complexes connecting epidermal basal cells to underlying basement membranes. The present study describes, for the first time, a method to prepare a fraction highly enriched with HD proteins from human material. Mass spectrometry and immunoblotting analyses demonstrated that the fraction contained newly identified proteins in addition to known HD components.

Research Products

• [Journal Article] Plectin deficiency leads to both muscular dystrophy and pyloric atresia in epidermolysis bullosa simplex. (2010)
  • Author(s): Natsuga K, Nishie W, Shinkuma S, Arita K, Nakamura H, Ohyama M, Osaka H, Kambara T, Hirako Y, Shimizu H.
  • Journal Title: Hum Mutat 31(10) Pages: 1687-1698
  • Peer Reviewed
• [Journal Article] Plectin expression patterns determine two distinct subtypes of epidermolysis bullosa simplex. (2010)
  • Author(s): Natsuga K, Nishie W, Akiyama M, Nakamura H, Shinkuma S, McMillan JR, Nagasaki A, Has C, Ouchi T, Ishiko A, Hirako Y, Owaribe K, Sawamura D, Bruckner-Tuderman L, Shimizu H.
  • Journal Title: Hum Mutat 31(3) Pages: 308-316
  • Peer Reviewed
• [Journal Article] Plectin deficiency leads to both muscular dystrophy and pyloric atresiain epidermolysis bullosa simplex. (2010)
  • Author(s): Natsuga Ken
  • Journal Title: Human Mutation Volume: 31 Pages: 1687-1698
  • Peer Reviewed
• [Journal Article] Plectin expression patterns determine two distinct subtypes of epidermolysis bullosa simplex. (2010)
  • Author(s): Natsuga Ken
  • Journal Title: Human Mutation 31 Pages: 308-316
  • Peer Reviewed
• [Journal Article] IgG from patients with bullous pemphigoid depletes cultured keratinocytes of the 180-kDa bullous pemphigoid antigen (type XVII collagen) and weakens cell attachment. (2009)
  • Author(s): Iwata H, Kamio N, Aoyama Y, Yamamoto Y, Hirako Y, Owaribe K, Kitajima Y.
  • Journal Title: J Invest Dermatol. 129(4) Pages: 919-926
  • Peer Reviewed
• [Journal Article] IgG from patients with bullous pemphigoid depletes cultured keratinocytes of the 180-kDa bullous pemphigoid antigen(type XVII collagen)and weakens cell attachment. (2009)
  • Author(s): Iwata Hiroaki
  • Journal Title: Journal of Investigative Dermatology 129 Pages: 919-926
  • Peer Reviewed
• [Journal Article] 細胞接着と細胞骨格 (2008)
  • Author(s): 平子善章, 尾張部克志
  • Journal Title: 生化学 80(3) Pages: 184-193
  • NAID: 10021191078
  • Peer Reviewed
• [Journal Article] 細胞接着と細胞骨格 (2008)
  • Author(s): 平子善章
  • Journal Title: 生化学 80 Pages: 184-193
  • NAID: 10021191078
  • Peer Reviewed
• [Presentation] A monoclonal antibody and Linear IgA bullous dermatosis autoantibodies preferentially recognize enitones within the NC16A domain of BP180 (2010)
  • Author(s): 松下知嗣
  • Organizer: 第35回日本研究皮膚科学会大会
  • Place of Presentation: 和歌山県民文化会館(和歌山県和歌山市)
  • Year and Date: 2010-12-03
• [Presentation] 消化管単層上皮におけるヘミデスモソーム関連タンパク質の分布パターン (2010)
  • Author(s): 富樫宏一
  • Organizer: 第42回日本結合組織学会学術大会・第57回マトリックス研究会大会合同学術集会
  • Place of Presentation: 秋田拠点センター・アルヴェ(秋田県秋田市)
  • Year and Date: 2010-08-20
• [Presentation] A monoclonal antibody and Linear IgA bullous dermatosis autoantibodies preferentially recognaize epitopes within the NC16A domain of BP180 (2010)
  • Author(s): 松下知嗣、尾張部克志、平子善章
  • Organizer: 第35回日本研究皮膚科学会大会
  • Place of Presentation: 和歌山
• [Presentation] Cell to matrix adhesion structures in stratified and simple epithelia (2009)
  • Author(s): 平子善章
  • Organizer: 第34回日本研究皮膚科学会大会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2009-12-04
• [Presentation] Calcium influences hemidesmosome formation and processing of laminin 332 (2009)
  • Author(s): 平子善章
  • Organizer: 8th Pan-Pacific Connective Tissue Society Symposium
  • Place of Presentation: 神奈川県・湘南国際村センター
  • Year and Date: 2009-06-05
• [Presentation] Cell to matrix adhesion structures in stratified and simple epithelia (2009)
  • Author(s): 平子善章
  • Organizer: 第34回日本研究皮膚科学会大会
  • Place of Presentation: 博多
• [Presentation] Analyses of hemidesmosome-rich fraction prepared from a human squamous cell carcinoma line (2009)
  • Author(s): 米元裕貴、桂朋矢、尾張部克志、平子善章
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] Preperation of the hemidesmosome-rich fractionfrom a human squamous cell carcinoma line (2008)
  • Author(s): 米元裕貴
  • Organizer: 回細胞生物学会大会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2008-07-01
• [Presentation] Gastrointestinal distribution of the 180-kDa bullous pemphigoid antigen (2008)
  • Author(s): 平子善章
  • Organizer: Post IID 2008 satellite international meeting on autoimmune bullous diseases
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2008-05-18
• [Presentation] Preparation of the hemidesmosome-rich fraction from a human squamous cell carcinoma line (2008)
  • Author(s): 米元裕貴、尾張部克志、平子善章
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
• [Remarks] ホームページ等