Comprehensive analysis of human hemidesmosomal proteins by mass spectrometry
| Project/Area Number |
20591341
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Nagoya University |
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Principal Investigator |
HIRAKO Yoshiaki Nagoya University, 理学研究科, 講師 (50377909)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細胞接着 / 表皮水疱症 / 自己免疫疾患 / ヘミデスモソーム / 質量分析 / 蛋白質 / 細胞・組織 / 生体分子 |
|---|
| Research Abstract |
Hemidesmosomes (HDs) are protein complexes connecting epidermal basal cells to underlying basement membranes. The present study describes, for the first time, a method to prepare a fraction highly enriched with HD proteins from human material. Mass spectrometry and immunoblotting analyses demonstrated that the fraction contained newly identified proteins in addition to known HD components.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Plectin expression patterns determine two distinct subtypes of epidermolysis bullosa simplex.2010
Author(s)
Natsuga K, Nishie W, Akiyama M, Nakamura H, Shinkuma S, McMillan JR, Nagasaki A, Has C, Ouchi T, Ishiko A, Hirako Y, Owaribe K, Sawamura D, Bruckner-Tuderman L, Shimizu H.
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Journal Title
Hum Mutat 31(3)
Pages: 308-316
Related Report
Peer Reviewed
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