Mechanisms of keratinocyte migration stimulated by laminin a3 chain-derived peptide : Its application for wound healing acceleration

• Project/Area Number: 20591344
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kyoto University
• Principal Investigator: UTANI Atsushi Kyoto University, 大学院・医歯薬学総合研究科, 教授 (10292707)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ラミニン / シンデカン / 創傷治癒 / ウミニン

Research Abstract

Laminin-5 (LN332) is a heterotrimeric basement membrane-specific glycoprotein composed of α3, β3, and γ2 subunits and involves in biological activities such as keratinocyte differentiation, proliferation, as well as a maintenance of stable basement membrane structure. Studies of in vivo wound tissue suggest that LN332 plays a role in facilitating cell migration during wound healing. In this study, we demonstrated that a synthetic peptide, PEP75, which contains the syndecan-binding sequence of the laminin α3LG4 module, induces keratinocyte migration in in vitro, ex vivo, and in vivo. To activate integrin function through syndecans by this synthetic peptide could be a novel therapeutic approach for chronic cutaneous wound.

Research Products

• [Journal Article] Laminin alpha3 chain-derived peptide promotes keratinocyte migration and wound closure: clustering of syndecan-4 and integrin beta1. (2010)
  • Author(s): Utani A.
  • Journal Title: Seikagaku 82(4) Pages: 327-331
• [Journal Article] Laminin alpha3 chain-derived peptide promotes keratinocyte migration and wound closure : clustering of syndecan-4 and integrin beta1 (2010)
  • Author(s): Utani A
  • Journal Title: Seikagaku. Volume: 82 Pages: 327-31
• [Journal Article] ミニレビュー:ラミニン5α3鎖由来ペプチドの細胞遊走および創傷治癒への関与:シンデカン4とインテグリンβ1凝集形成 (2010)
  • Author(s): 宇谷厚志
  • Journal Title: 日本生化学学会 (印刷中)(未定)
• [Journal Article] Clustering of syndecan-4 and integrin b1 by laminin a3 chain-derived peptide promotes keratinocyte migration. (2009)
  • Author(s): Araki E, Momota Y, Togo T, Tanioka M, Hozumi K, Nomizu M, Miyachi Y, Utani A.
  • Journal Title: Mol Biol Cell 20(13) Pages: 3012-3024
• [Presentation] シンポジウム「組織を支える基底膜分子の機能解明と展望」 (2008)
  • Author(s): 宇谷厚志
  • Organizer: 40回日本結合組織学会学術大会・第55回マトリックス研究会大会 合同学術集会
  • Place of Presentation: 東京
• [Presentation] Syndecan-binding peptide PEP75 derived from laminin a3 stimulates integrin b1 dependent cell migration and accelerates wound healing in vivo. (2008)
  • Author(s): Atsushi Utani, Eri Araki, Yutaka Momota, Takeshi Togo, Yoshiki Miyachi
  • Organizer: Cell adhesion/Matrix biology, Minisymposium, the 5th International Invetigative Dermatology Kyoto 2008, 128, S1, S43, 2008
  • Place of Presentation: J Invest Dermatol
• [Presentation] Syndecan-binding peptide PEP75 derived from laminin a3 stimulates integrin bl dependent cell migration and accelerates wound healing in vivo (2008)
  • Author(s): Atsushi Utani, Eri Araki, Yutaka Momota, Takeshi Togo, Yoshiki Miyachi
  • Organizer: International Investigative Dermatology
  • Place of Presentation: Kyoto