| Project/Area Number |
20591359
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
YAMANISHI Kiyofumi Hyogo College of Medicine, 医学部, 教授 (10182586)
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| Co-Investigator(Kenkyū-buntansha) |
TARUTANI Masahito 高知大学, 医学部, 准教授 (30301261)
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| Research Collaborator |
TSUDA Tatsuya 兵庫医科大学, 医学部, 助教 (80434942)
IMAI Yasutomo 兵庫医科大学, 医学部, 助教 (10529514)
NAKAGAWA Noboru 兵庫医科大学, 医学部, 助教 (90412014)
YAMAMOTO Masaaki 兵庫医科大学, 大学院生
KONISH Hiroe 兵庫医科大学, 実験補助
SAKAGUCHI Yoshiko 兵庫医科大学, 実験補助
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| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 角層 / タンパク質架橋 / トランスグルタミナーゼ / Gln-Lys結合 / SS結合 / 角化症 / 酵素 / 基質 / 角化細胞 / 遺伝子発現 / 組織化学 / 遺伝子変異 / 皮膚 |
|---|
| Research Abstract |
The protein cross-links in the stratum corneum include Gln-Lys and SS bonds. In the present study, in combination of the high resolution fluorescent imaging for in situ transglutaminase activity and SS bonds with immunofluorescence, we showed that those cross-links are formed in order in the keratinization process to reinforce the structure of the stratum corneum. Furthermore, we demonstrated that transglutaminase 1 is inevitable for the proper localization of SS in the stratum corneum using mutant mice in transglutaminase 1.
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