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Transglutaminase and protein polymerization in keratinization

Research Project

Project/Area Number 20591359
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionHyogo College of Medicine

Principal Investigator

YAMANISHI Kiyofumi  Hyogo College of Medicine, 医学部, 教授 (10182586)

Co-Investigator(Kenkyū-buntansha) TARUTANI Masahito  高知大学, 医学部, 准教授 (30301261)
Research Collaborator TSUDA Tatsuya  兵庫医科大学, 医学部, 助教 (80434942)
IMAI Yasutomo  兵庫医科大学, 医学部, 助教 (10529514)
NAKAGAWA Noboru  兵庫医科大学, 医学部, 助教 (90412014)
YAMAMOTO Masaaki  兵庫医科大学, 大学院生
KONISH Hiroe  兵庫医科大学, 実験補助
SAKAGUCHI Yoshiko  兵庫医科大学, 実験補助
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords角層 / タンパク質架橋 / トランスグルタミナーゼ / Gln-Lys結合 / SS結合 / 角化症 / 酵素 / 基質 / 角化細胞 / 遺伝子発現 / 組織化学 / 遺伝子変異 / 皮膚
Research Abstract

The protein cross-links in the stratum corneum include Gln-Lys and SS bonds. In the present study, in combination of the high resolution fluorescent imaging for in situ transglutaminase activity and SS bonds with immunofluorescence, we showed that those cross-links are formed in order in the keratinization process to reinforce the structure of the stratum corneum. Furthermore, we demonstrated that transglutaminase 1 is inevitable for the proper localization of SS in the stratum corneum using mutant mice in transglutaminase 1.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (27 results)

All 2011 2010 2009 2008 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (6 results) Book (4 results) Remarks (1 results)

  • [Journal Article] Lichen planus-like dermatosis distributed along the lines of Blaschko.2011

    • Author(s)
      Yamamoto M, Imai Y, Nakagawa N, Yamanishi K
    • Journal Title

      J Dermatol. 38

      Pages: 190-1

    • NAID

      10029330435

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In Situ Detection of Active Transglutaminases for Keratinocyte-type (TGase 1) and Tissue-type (TGase 2) Using Fluorescence-labeled Highly Reactive Substrate Peptides.2011

    • Author(s)
      Itoh M, Kawamoto T, Tatsukawa H, Kojima S, Yamanishi K, Hitomi K
    • Journal Title

      J Histochem Cytochem. 59

      Pages: 180-187

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lichen planus-like dermatosis distributed along the lines of Blaschko.2011

    • Author(s)
      Yamamoto M, et al.
    • Journal Title

      Journal of Dermatology

      Volume: 38 Pages: 190-191

    • NAID

      10029330435

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In situ detection of active transglutaminases for keratinocyte type (TGase 1) and tissue type (TGase 2) using fluorescence-labeled highly reactive substrate peptides.2011

    • Author(s)
      Itoh M, et al.
    • Journal Title

      Journal of Histochemistry and Cytochemistry

      Volume: 59 Pages: 180-187

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neutrophil-dominant psoriasis-like skin inflammation induced by epidermal-specific expression of Raf in mice.2010

    • Author(s)
      Tarutani M, Imai Y, Yasuda K, Tsutsui H, Nakanishi K, Yamanishi K
    • Journal Title

      J Dermatol Sci. 58

      Pages: 28-35

    • NAID

      10027400286

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neutrophil-dominant psoriasis-like skin inflammation induced by epidermal-specific expression of Raf in mice.2010

    • Author(s)
      Tartutani M, et al.
    • Journal Title

      Journal of Dermatological Science

      Volume: 59 Pages: 64-71

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tetracyclines modulate protease-activated receptor 2-mediated proinflammatory reactions in epidermal keratinocytes.2009

    • Author(s)
      Ishikawa C, Tsuda T, Konishi H, Nakagawa N, Yamanishi K
    • Journal Title

      Antimicrob Agents Chemother 53

      Pages: 1760-1765

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cutaneous malignant fibrous histiocytoma (undifferentiated pleomorphic sarcoma) arising in a chronic scalp ulcer of a patient with non-bullous congenital ichthyosiform erythroderma.2009

    • Author(s)
      Fujimoto T, Tsuda T, Yamamoto M, Tarutani M, Natsuaki M, Minami S, Ito T, Kozuka T, Yamanishi K
    • Journal Title

      J Eur Acad Dermatol Venereol 23

      Pages: 202-203

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cutaneous malignant fibrous histiocytoma(undifferentiated pleomorphic sarcoma)arising in a chronic scalp ulcer of a patient with non-bullous congenital ichthyosiform erythroderma.2009

    • Author(s)
      Fujimoto T, et al.
    • Journal Title

      Journal of European Academy of Dermatology and Venereology 23

      Pages: 202-203

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tetracyclines modulate protease-activated receptor 2-mediated proinflammatory reactions in epidermal keratinocytes.2009

    • Author(s)
      Ishikawa C, et al.
    • Journal Title

      Antimicrobial Agents and Chemotherapy 53

      Pages: 1760-1765

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of preferred substrate sequences for transglutaminase 1-development of a novel peptide that can efficiently detect cross-linking enzyme activity in the skin.2008

    • Author(s)
      Sugimura Y, Hosono M, Kitamura M, Tsuda T, Yamanishi K, Maki M, Hitomi K
    • Journal Title

      FEBS J 275

      Pages: 5667-5677

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel point mutation of keratin 17 (KRT17) in a Japanese family with pachyonychia congenita type 2 : An RNA-based genetic analysis using a single hair bulb.2008

    • Author(s)
      Tsuda T, Ishikawa C, Nakagawa N, Konishi H, Tarutani M, Matsuki M, Yamanishi K
    • Journal Title

      Br J Dermatol 159

      Pages: 730-732

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of 14-membered ring macrolides on the production of interleukin-8 mediated by protease-activated receptor 2 in human keratinocytes.2008

    • Author(s)
      Tsuda T, Ishikawa C, Konishi H, Hayashi Y, Nakagawa N, Matsuki M, Mizutani H, Yamanishi K
    • Journal Title

      Antimicrob Agents Chemother 52

      Pages: 1538-1541

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel point mutation of keratin 17 (KRT17) in a Japanese family with pachyonychia congenita type 2 : An RNA-based genetic analysis using a single hair bulb.2008

    • Author(s)
      Tsuda T, et al.
    • Journal Title

      British Journal of Dermatology 159

      Pages: 730-732

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of preferred substrate sequences for transglutaminase 1-development of a novel peptide that can efficiently detect cross -linking enzyme activity in the skin.2008

    • Author(s)
      Sugimura Y, et al.
    • Journal Title

      FEBS Journal 275

      Pages: 5667-5677

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effect of 14-menibered ring macrolides on the production of interleukin-8 mediated by protease-activated receptor 2 in human keratinocytes.2008

    • Author(s)
      Tsuda T, et al.
    • Journal Title

      Antimicrob Agents Chemother 52

      Pages: 1538-1541

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] An unusual Japanese case of congenital ichthyosis with TGM1 mutations : A variant of bathing suit ichthyosis?2010

    • Author(s)
      Nakagawa N., et al.
    • Organizer
      第10回イオニア国際皮膚科学会議
    • Place of Presentation
      マルタ共和国セントポールベイ
    • Year and Date
      2010-10-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] An unusual Japanese case of congenital ichthyosis with TGM1 mutations : A variant of bathing suit ichthyosis?2010

    • Author(s)
      Nakagawa N., Yamamoto M, Sakaguchi Y, Imai Y, Yamanishi K
    • Organizer
      The 10th Ionic Dermatological Association (ADI) International Congress
    • Place of Presentation
      St. Paul's bay, Malta
    • Related Report
      2010 Final Research Report
  • [Presentation] Stratum corneum and homeostasis of the cutaneous barrier.2009

    • Author(s)
      Yamanishi K
    • Organizer
      The 1st international symposium of deimination and skin biology.
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2009-04-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Stratum corneum and homeostasis of the cutaneous barrier.2009

    • Author(s)
      Yamanishi K.
    • Organizer
      The 1st international symposium of deimination and skin biology.
    • Place of Presentation
      Rihga Royal Hotel(Osaka)
    • Year and Date
      2009-04-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Psoriasis-like hyprplastic and inflammatory lesions produced by epidermis-specific inducible activation of Raf in mice.2008

    • Author(s)
      Tarutani M, et al.
    • Organizer
      International Investigative Dermatology 2008
    • Place of Presentation
      京都
    • Year and Date
      2008-05-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] Psoriasis-like hyprplastic and inflammatory lesions produced by epidermis-specific inducible activation of Raf in mice.2008

    • Author(s)
      Tarutani M, Imai Y, Tsuda T, Nakanishi K, Yamanishi K
    • Organizer
      International Investigative Dermatology
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2010 Final Research Report
  • [Book] 皮膚疾患診療実践ガイド 第2版2009

    • Author(s)
      山西清文,他
    • Total Pages
      856
    • Publisher
      文光堂
    • Related Report
      2010 Final Research Report
  • [Book] 皮膚疾患診療実践ガイド 第2版2009

    • Author(s)
      山西清文, 他
    • Total Pages
      856
    • Publisher
      文光堂
    • Related Report
      2009 Annual Research Report
  • [Book] 角化異常/紅斑・紅皮症/紫斑/血管(皮膚科診療カラーアトラス体系 第2巻)2008

    • Author(s)
      山西清文,他
    • Total Pages
      184
    • Publisher
      講談社
    • Related Report
      2010 Final Research Report
  • [Book] 皮膚科診療カラーアトラス体系第2巻「角化異常/紅斑・紅皮症/紫斑/血管」2008

    • Author(s)
      山西清文, ほか
    • Total Pages
      184
    • Publisher
      講談社
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.hyo-med.ac.jp/department/drmt/Ref1.html

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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