Hippocampal Gene expression analysis related with redox regulation in excitatory synaptic circuit of epileptogenesis

• Project/Area Number: 20591372
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: University of Miyazaki
• Principal Investigator: UEDA Yuto University of Miyazaki, 医学部, 准教授 (70244192)
• Co-Investigator(Kenkyū-buntansha): DOI Taku 宮崎大学, 医学部, 助教 (70274793) ; NAKAJIMA Akira 宮崎大学, 医学部, 准教 (10041857)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: てんかん / グルタミン酸 / GABA / トランスポーター / レドックス制御 / チオレドキシン / レドックス抑制

Research Abstract

As well known the knockdown EAAC-1 by anti-sense induces epileptic convulsion in rats, EAAC-1 is important protein to connect glutamate re-uptake with GABA synthesis. It is important study to elucidate the role of glutamate transporter associated protein 3-18 (GTRAP3-18), because GTRAP3-18 inhibitory regulates the glutamate re-uptake through EAAC-1 into GABAergic neurons. Hippocampal glutamate and GABA basal release in GTRAP3-18 knockdown group higher rather than those of sense-injected group suggested knockdown of GTRAP3-18 promotes GABA synthesis. Sustenance of high seizure susceptibility in the PTZ kindled state and the existence of inter-paroxysmal period could be explained by synchronization of glutamatergic neuron caused by the enhanced GABAergic system.

Research Products

• [Journal Article] Differential molecular regulation of glutamate in kindling resistant rats. (2011)
  • Author(s): Doi T, Ueda Y, Takaki M, Willmore LJ
  • Journal Title: Brain Res. Volume: 1375 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Hydrogen-related enhancement of in vivo antioxidant ability in the brain of rats fed coral calcium hydride (2010)
  • Author(s): Ueda Y, Nakajima A, Oikawa T
  • Journal Title: Neurochem Res. Volume: 35(10) Pages: 1510-5
  • Peer Reviewed
• [Journal Article] Alterations in the hippocampal glycinergic system in an animal model of posttraumatic stress disorder. (2010)
  • Author(s): Yamamoto S, Morinobu S, Iwamoto Y, Ueda Y, Takei S, Fujita Y, Yamawaki
  • Journal Title: J Psychiatr Res Volume: 44(15) Pages: 1069-74
  • NAID: 120002753738
  • Peer Reviewed
• [Journal Article] 2010精神疾患治療の話題■てんかんの治療戦略■ (2010)
  • Author(s): 植田勇人
  • Journal Title: Medicament News Pages: 4-5
• [Journal Article] Levetiracetam enhances endogenoantioxidant in the hippocampusrats : in vivo evaluation by brain microdialysis combined with ESR spectroscopy. (2009)
  • Author(s): Ueda, Y., Doi, T., Takaki, M., Nagatomo, K., Nakajima, A., Willmore, L.J.
  • Journal Title: Brain Res 1266 Pages: 1-7
• [Journal Article] パーキンソン病におけるレドックス (2009)
  • Author(s): 植田勇人
  • Journal Title: 自律神経 46 Pages: 181-185
  • NAID: 10025571061
• [Journal Article] 【抗てんかん薬】レベチラセタム (2009)
  • Author(s): 植田勇人
  • Journal Title: 最新精神医学 14 Pages: 355-359
• [Journal Article] (2009)
  • Author(s): Ueda, Y., Willmore, L.J.
  • Journal Title: Role of Transporter Proteins and Lipid Peroxidation in Epileptoge nesis(Encyclopedia of Basic Epilepsy Research. Academic Press, Oxford, 2009;) Pages: 250-253
• [Journal Article] Role of Glutamate and GABA Transporters in Development of Pent : ylenetetrazol-Kindling. (2009)
  • Author(s): Doi, T., Ueda, Y., Nagatomo, K., Willmore, L. J.
  • Journal Title: Neurochem Res. On-linepublished Pages: 1324-1331
  • Peer Reviewed
• [Journal Article] Levetiracetam enhances endogenous antioxidant in the hippocamp us of rats : In vivo evaluation by brain microdialysis combined with ESR spectroscopy (2009)
  • Author(s): Ueda, Y., Doi, T., Takaki, M., Nagatomo, K., Nakajima, A., Willmore, L. J.
  • Journal Title: Brain Res 1266 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Redox and neuroprotection. (2008)
  • Author(s): Ueda, Y.
  • Journal Title: J Brain Science. 34 Pages: 1-5
• [Journal Article] Molecular regulation of antioxidant ability in the hippocampus of EL mice. (2008)
  • Author(s): Takaki, M., Ueda, Y., Doi, T., Nagatomo, K., Murashima, Y. L., Kannan, H.
  • Journal Title: Brain Res. 1228, 1-5. 1228 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Redox regulation and anti-convulsive effect of zonisamide. (2007)
  • Author(s): Ueda, Y.
  • Journal Title: Redox and ZNS 1 Pages: 1-3
• [Presentation] Hippocampal gene network analysis to determine the effects of antioxidant treatment in an experimental model of accelerated senescence (2010)
  • Author(s): Ueda, Y., Sato, A., Kitamoto, A., Adati, N., Oikawa, T., Kojima, T.
  • Organizer: In : Inflammation 2010.
  • Place of Presentation: Luxembourg.
  • Year and Date: 2010-11-18
• [Presentation] Hippocampal gene network analysis to determine the effects of antioxidant treatment in an experimental model of accelerated senescence (2010)
  • Author(s): Ueda, Y
  • Organizer: Cell Signal-omits
  • Place of Presentation: Luxembourg
  • Year and Date: 2010-11-18
• [Presentation] Affect of dissolved oxygen on the reduction of nitroxide by vitamin C : In vitro model for acute ischamia. (2010)
  • Author(s): Nakajima, A., Masuda, Y., Matsuda, E., Ueda, Y., Sameshima, H., Ikenoue, T.
  • Organizer: In : APES2010.
  • Place of Presentation: Jeju, Korea.
• [Presentation] Affect of dissolved oxygen on the reduction of nitroxide by vitamin C : In vitro model for acute ischamia. (2010)
  • Author(s): Nakajima, N., Masuda, Y., Matsuda, E., Ueda, Y., Samejima, H., Ikenoue, T.
  • Organizer: In : APES2010.
  • Place of Presentation: Jeju.
• [Presentation] Gene network analysis the effects of coral calcium hydride treatment to senescence accelerated mice. (2010)
  • Author(s): Ueda, Y., Kojima, T., Oikawa, T.
  • Organizer: In : The 14th International Congress of Immunology
  • Place of Presentation: Kobe
• [Presentation] Gene network analysis the effects of coral calcium hydride treatment to senescence accelerated mice. (2010)
  • Author(s): Ueda, Y
  • Organizer: 弟14回国際免疫学会議
  • Place of Presentation: 神戸(兵庫県)
• [Presentation] Differential latency between glutamate and GABA release with seizures induced by NMDA. (2009)
  • Author(s): Chihara, Y., Ueda, Y., Doi, T., Willmore, L.J.
  • Organizer: In : 63th American Epilepsy Society's Annual Meeting.
  • Place of Presentation: Boston.
• [Presentation] Differential molecular regulation of glutamate in kindling resistant rats. (2009)
  • Author(s): Doi, T., Ueda, Y., Chihara, Y., Willmore, L.J.
  • Organizer: In : 63th American Epilepsy Society's Annual Meeting.
  • Place of Presentation: Boston
• [Presentation] Down-regulated anti-oxidant ability and seizure sensitivity of mature rats after neonatal hypoxic-ischemic brain injury (2009)
  • Author(s): Ueda, Y., Doi, T., Chihara, Y., Nakajima, A., Willmore, L.J.
  • Organizer: In : 63th American Epilepsy Society's Annual Meeting.
  • Place of Presentation: Boston.
• [Presentation] Down-regulated anti-oxidant ability and seizure sensitivity of mature rats after neonatal hypoxic-ischemic brain injury Boston, 2009 (2009)
  • Author(s): Ueda, Y., Doi, T., Chihara, Y., Nakajima, A., Willmore, L.J.
  • Organizer: 63th American Epilepsy Society's Annual Meeting
  • Place of Presentation: Boston
• [Presentation] パーキンソン病とレドックス (2009)
  • Author(s): 植田勇人, 中島暉, 土井拓
  • Organizer: 日本脳科学会
  • Place of Presentation: 金沢