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The investigation to Dynamin-1 for new antidepressats binding protein

Research Project

Project/Area Number 20591380
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionSt.Marianna University School of Medicine

Principal Investigator

OSADA Kenichi  St.Marianna University School of Medicine, 医学部, 講師 (20233504)

Co-Investigator(Kenkyū-buntansha) YAMADA Mituhiko  国立精神・神経センター 精神保健研究所, 精神薬理研究部, 部長 (60240040)
KIDA Satoshi  東京農業大学, 用生物科学部, 教授 (80301547)
MIYOSHI Hiroshi  聖マリアンナ医科大学, 医学部, 講師 (80322519)
Co-Investigator(Renkei-kenkyūsha) ASAKURA Mikio  聖マリアンナ医科大学, 医学部, 准教授 (70103504)
MATUI Hiroaki  聖マリアンナ医科大学, 医学(系)研究科(研究院), 教授 (90181685)
KATOU Tomohiro  聖マリアンナ医科大学, 医学部, 教授 (80233807)
SASUGA Yasuo  聖マリアンナ医科大学, 医学部, 助教 (60308450)
MISONOO Atushi  聖マリアンナ医科大学, 医学部, 助教 (20410128)
GAWA Yuriko  聖マリアンナ医科大学, 医学部, 研究技術員 (90449398)
TANAKA Daisuke  聖マリアンナ医科大学, 医学部, 助教 (80410124)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsSNRI / dynamin / GTPase
Research Abstract

Dynamin (Dyn) 1 plays a role in recycling of synaptic vesicles, and thus in nervous system function. Fluvoxamine , a selective serotonin reuptake inhibitor (SSRI),may compete with L-phosphatidylserine (PS) for binding to the pleckstrin homology domain of dynamin I (Dyn 1) GTPase. On the other hand, sertraline was a mixed type inhibitor with respect to both GTP and PS. We showed that sertraline is a mixed-type inhibitorof Dyn 1 with respect to both GTP and L-a-phosphatidyl-L-serine (PS) in vitro, and we suggested that it may regulate the neurotransmitter transport by modulating synaptic vesicle endocytos is via inhibition of Dyn 1 GTPase. GTPase assay showed that sertraline inhibited Dyn 2 as well as Dyn 1. Therefore, the effect of sertraline on endocytosis was mediated by Dyn 2, at least in HeLa cells, as well as by Dyn 1 in cell lines that express it. Moreover, the inhibition mechanism of transferrin (Tf) uptake by sertraline differed from that in cells expressing Dyn 1 K44A, a GTP binding-defective variant.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report

Research Products

(34 results)

All 2011 2010 2009 2008 Other

All Journal Article (20 results) (of which Peer Reviewed: 15 results) Presentation (10 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Development of the Japanese version of the Fibromyalgia Impact Questionnaire (JFIQ) : The assessments of reliability and validity2011

    • Author(s)
      Osada K, Oka H, Isomura T, Nakamura I, Tominaga K, Takahashi S, Kojima A, Nishioka K
    • Journal Title

      Int.J.Rheumatol Dis 14(1)

      Pages: 74-80

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of the Japanese version of the Fibromyalgia Impact Questionnaire (JFIQ)2011

    • Author(s)
      K.Osada, et at.
    • Journal Title

      Int.J.Rheumatic Diseases

      Volume: 14(1) Pages: 74-80

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chronic trifluoperazine treatment increased P-glycoprotein in the rat brain.2010

    • Author(s)
      Osada K, Ogawa Y, Haga T, Nakano M, Tanaka D, Sasuga Y, Asakura M, Yamaguchi N
    • Journal Title

      J.St.Marianna Univ 38(1)

      Pages: 7-13

    • NAID

      40018813569

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of dynamin GTPase activity by sertraline leads to inhibition of dynamin-dependent endocytosis.2010

    • Author(s)
      Takahashi K, Miyoshi H, Otomo M, Osada K, Yamaguchi N, Nakashima H
    • Journal Title

      Biochem Bio Res Comm 391

      Pages: 382-387

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fluvoxamine and sigma-1 receptor agonists dehydroepiandrosterone (DHEA)-sulfate induces the Ser473-phosphorylation of Akt-1 in PC12 cells.2010

    • Author(s)
      Nakano M, Osada K, Misonoo A, Fujiwara K, Takahashi M, Ogawa Y, Haga T, Kanai S, Tanaka D, Sasuga Y. Yanagida T, Asakura M, Yamaguchi N
    • Journal Title

      Life Sciences 86

      Pages: 309-314

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of dynamin GTPase activity by sertraline leads to inhibition of dynamin-dependent endocytosis2010

    • Author(s)
      K.Takahashi, et al.
    • Journal Title

      Biochem Biophy Resear Comm

      Volume: 391 Pages: 382-387

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluvoxamine and sigma-1 receptor agonists dehydroepiandrosterone (DHEA)-sulfate induces the Ser473- phosphorylation of Akt-1 in PC12 cells2010

    • Author(s)
      M.Nakano, et al.
    • Journal Title

      Life science

      Volume: 86 Pages: 309-314

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chronic trifluoperazine treatment increased P-glycoprotein in the rat brain.2010

    • Author(s)
      K.Osada, et al.
    • Journal Title

      J.St.Marianna Univ

      Volume: 38(1) Pages: 7-13

    • NAID

      40018813569

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 精神科領域からみた線維筋痛症2010

    • Author(s)
      長田賢一, 他
    • Journal Title

      神経内科

      Volume: 72(5) Pages: 486-493

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Suppression of dynamin GTPase activity by sertraline leads to inhibition of dynamin-dependent endocytosis2010

    • Author(s)
      K.Takahashi, et al.
    • Journal Title

      Biochem Biophy Resear Comm 391

      Pages: 382-387

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluvoxamine and sigma-1 receptor agonists dehydroepiandrosterone(DHEA)-sulfate induces the Ser473-phosphorylation of Akt-1 in PC12 cells2010

    • Author(s)
      M.Nakano, et al.
    • Journal Title

      Life science 86

      Pages: 309-314

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blonaserin長期投与試験の臨床評価2009

    • Author(s)
      長田賢一
    • Journal Title

      臨床精神薬理 12

      Pages: 2622-2629

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 統合失調症に対するblonaserinの長期投与試験2009

    • Author(s)
      長田賢一, 他
    • Journal Title

      臨床精神薬理 12

      Pages: 2337-2351

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chronic treatment with fluvoxaime stimulates phosophorylation of Ser473 anf Thr308 of AKT in the rat cerebral cortex.2008

    • Author(s)
      Misonoo A., Osada K, Nakano M.
    • Journal Title

      聖マリアンナ医科大学大学雑誌 36(3)

      Pages: 207-214

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Some selective serotonin reuptake inhibitors inhibit dynamin I GTPase.2008

    • Author(s)
      Otomo M, Takahashi K, Miyoshi H, Osada K, Nakashima H, Yamaguchi N
    • Journal Title

      Biol Pharm Bull 88

      Pages: 1489-1495

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 向精神薬の脳内濃度の調節するP糖蛋白質について2008

    • Author(s)
      長田 賢一, 他
    • Journal Title

      精神科 12

      Pages: 434-439

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Chronic treatment with fluvoxaime stimulates phosophorylation of Ser473 anf Thr308 of ART in the rat cerebral cortex2008

    • Author(s)
      A. Misonoo, et al.
    • Journal Title

      聖マリアンナ医科大学大学雑誌 36

      Pages: 207-214

    • Related Report
      2008 Annual Research Report
  • [Journal Article] P糖蛋白質-向精神薬の脳内濃度の調節について-2008

    • Author(s)
      長田 賢一, 他
    • Journal Title

      分子精神医学 8

      Pages: 83-85

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Some selective serotonin reuptake inhibitors inhibit dynamin IGTPase2008

    • Author(s)
      Otomo, M., et al.
    • Journal Title

      Biol. Pharm. Bull. 88

      Pages: 1489-1495

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 日本語版Fibromyalgia Impact Quesionnarire (JFIQ)の開発 : 言語的妥当性を担保した翻訳版の作成2008

    • Author(s)
      長田 賢一, 他
    • Journal Title

      臨床リウマチ 20

      Pages: 19-28

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Fluvoxamine and Sigma-1 Receptor Agonists Dehydroepiandrosterone (DHEA)-sulfate Induces the Ser473-phosphorylation of Akt-1 in PC12 Cells.2010

    • Author(s)
      Osada K, Haga T, Ogawa Y, Matsui H, Nakano M, Asakura M, Yamaguchi N
    • Organizer
      41th Annual Meeting of Neuroscience
    • Year and Date
      2010-11-14
    • Related Report
      2010 Final Research Report
  • [Presentation] Fluvoxamine and Sigma-1 Receptor Agonists Dehydroepiandrosterone (DHEA)-sulfate Induces the Ser473-phosphorylation of Akt-1 in PC12 Cells.2010

    • Author(s)
      K.Osada, et al.
    • Organizer
      41th Annual Meeting of Neuroscience
    • Place of Presentation
      サンデイエゴ(U.S.A)
    • Year and Date
      2010-11-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] Validation of a fluorescence-based high-thoughput method for the cellualar serotonin uptake activity2009

    • Author(s)
      T.Haga, et al.
    • Organizer
      38rd Annual Meeting of Neuroscience
    • Place of Presentation
      Chicago
    • Year and Date
      2009-10-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] Chronic trifluoperazine treatment increased P-glycoprotein in the rat brain.2009

    • Author(s)
      Osada K, Ogawa Y, Matsui H, Haga T, Nakano M, Takahashi K, Fujiwara K, Yanagida T, Kanai S
    • Organizer
      40th Annual Meeting of Neuroscience
    • Year and Date
      2009-10-19
    • Related Report
      2010 Final Research Report
  • [Presentation] Chronic trifluoperazine treatment increased P-glycoprotein in the rat brain2009

    • Author(s)
      K.Osada, et al.
    • Organizer
      38rd Annual Meeting of Neuroscience
    • Place of Presentation
      Chicago
    • Year and Date
      2009-10-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Fuorescence-based high-thoughput method for milnasipran in vivo cells2009

    • Author(s)
      Y.Ogawa, et al.
    • Organizer
      38rd Annual Meeting of Neuroscience
    • Place of Presentation
      Chicago
    • Year and Date
      2009-10-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Neurotransmitter (serotonin, dopamine, noradrenaline )were labeled NBD-F and detected fluorescence in vivo2008

    • Author(s)
      T. Haga, et al.
    • Organizer
      38rd Annual Meeting of Neuroscience
    • Place of Presentation
      Washington(U. S. A)
    • Year and Date
      2008-11-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] Antidepressants induces phosphorylation of Akt in PC122008

    • Author(s)
      K. Osada, et al.
    • Organizer
      38rd Annual Meeting of Neuroscience
    • Place of Presentation
      Washington(U. S. A)
    • Year and Date
      2008-11-17
    • Related Report
      2008 Annual Research Report
  • [Presentation] Fluorescence-based high-throughput method for Milnacipran in vivo cells2008

    • Author(s)
      Y. Ogawa, et al.
    • Organizer
      38rd Annual Meeting of Neuroscience
    • Place of Presentation
      Washington(U. S. A)
    • Year and Date
      2008-11-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] Antidepressants induces phosphorylation of Akt in PC12.2008

    • Author(s)
      Osada K, Nakano M, Misonoo A, Kanai S, Tanaka D, Ootmono M, Sasuga Y, Takahashi K. Ogawa Y, Haga T, Asakura M
    • Organizer
      38rd Annual Meeting of Neuroscience
    • Year and Date
      2008-11-15
    • Related Report
      2010 Final Research Report
  • [Book] 向精神薬などの精神科的治療、線維筋痛症診療ガイドライン2009(厚生労働省研究班監修 東京)2010

    • Author(s)
      長田賢一
    • Publisher
      日本リウマチ財団
    • Related Report
      2010 Final Research Report
  • [Book] SNRIのすべて第二版(小山司・樋口輝彦編集)(疼痛に対するSNRIの臨床応用:線維筋痛症 東京)2010

    • Author(s)
      長田賢一
    • Publisher
      先端医学社
    • Related Report
      2010 Final Research Report
  • [Book] 線維筋痛症診療ガイドライン20092010

    • Author(s)
      長田賢一
    • Total Pages
      103
    • Publisher
      日本リウマチ財団
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://mariannapsycho.web.fc2.com/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-03-31   Modified: 2016-04-21  

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