| Project/Area Number |
20591517
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
KUZUYA Takafumi Nagoya University, 医学部附属病院, 副部長 (00444406)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Takaaki 名古屋大学, 大学院・医学系研究科, 教授 (70314010)
HANEDA Masataka 名古屋大学, 大学院・医学系研究科, 講師 (50436995)
KODERA Yasuhiro 名古屋大学, 大学院・医学系研究科, 准教授 (10345879)
IWASAKI Kenta 名古屋大学, 大学院・医学系研究科, 助教 (10508881)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 免疫抑制薬 / 薬力学 / IL-2 / 細胞増殖抑制効果 / 免疫卸制薬 / CFSE / Bリンパ球 / PCNA / インターロイキン2 / real-time RT-PCR / IC50値 |
|---|
| Research Abstract |
To optimize immunosuppressive treatment in individual patients, many biomarkers have been proposed, but there has been very little information about association to clinical outcome data. The most effective immunosuppressive strategies in organ transplantation are based on the inhibition of IL-2 signal by calcineurin inhibitiors and we first evaluated the inhibition of IL-2 mRNA production for each patient by using RT-PCR method. Then we examined individual T-cell sensitivity to each immunosuppressive agent (cyclosporine, tacrolimus, mycophenolic acid, predonisolone) using flow cytometry with CFSE labeling. Patients with high sensitivity to cyclosporine tended to experience viral reactivation. Pretransplant pharmacodynamics could provide useful information on optimal and safe immunosuppressive therapy.
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