Restoration of self liver using transplantation of autologous cryopreserved hepatocytes.
| Project/Area Number |
20591529
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
EGUCHI Susumu Nagasaki University, 大学院・医歯薬学総合研究科, 准教授 (80404218)
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| Co-Investigator(Kenkyū-buntansha) |
KANEMATSU Takashi 長崎大学, 大学院・医歯薬学総合研究科, 教授 (40128004)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 肝細胞 / 幹細胞 / 自己再生型 / 肝細胞移植 / 肝移植 / 移植・再生医療 |
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| Research Abstract |
This year we isolated human hepatocytes from resected liver specimen from patients with metastatic liver cancer. After some modification of the method of isolation, we could obtain intact human hepatocytes for transplantation. We transplanted those hepatocytes in nude mice and SCID mice.In addition, we used our original method to proliferate transplanted hepatocyte using radiation on host liver. Using those technique, after growth advantage, transplated human hepatocytes proliferate very well until replace a host liver to 95% By the time of 16 weeks. Around transplanted human hepatocytes, gap junction and glycogenesis were observed. Although auto transplantation has not been performed, concept of hepatocyte banking and retransplantation could be a good therapeutic choice for end stage liver diseases.
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Report
(4 results)
Research Products
(69 results)
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[Journal Article] Macrophage-dominant sialadenitis inhuman T-cell leukemia virus type I-associated yelopathy after living-donor liver transplantation.2010
Author(s)
Nakamura H, Ichikawa T, Nakamura T, Kawakami A, Iwamoto N, Matsuzaki T, Miyaaki H, Yamasaki S, Ida H, Eguchi S,Hayashi T, Nakao K, Kanematsu T, Eguchi K.
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Journal Title
Transplantation Proceedings. 42
Pages: 2797-2799
Related Report
Peer Reviewed
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[Journal Article] Macrophage-dominant sialadenitis in human T-cell leukemia virus type I-associated myelopathy after living-donor liver transplantation.2010
Author(s)
Nakamura H, Ichikawa T, Nakamura T, Kawakami A, Iwamoto N, Matsuzaki T, Miyaaki H, Yamasaki S, Ida H, Eguchi S, Hayashi T, Nakao K, Kanematsu T, Eguchi K.
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Journal Title
Transplantation Proceedings
Volume: 42
Pages: 2797-2799
NAID
Related Report
Peer Reviewed
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[Journal Article] Multicentric occurrence and spread of hepatocellular carcinoma in whole explanted end-stage liver.2009
Author(s)
Hidaka M, Eguchi S, Okudaira S, Takatsuki M, Tokai H, Soyama A, Nagayoshi S, Mochizuki S, Hamasaki K, Tajima Y, Kanematsu T.
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Journal Title
Hepatology Research 39
Pages: 143-148
NAID
Related Report
Peer Reviewed
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[Journal Article] Acute deterioration of idiopathic portal hypertension requiring living donor liver transplantation : a case report.2009
Author(s)
Inokuma T, Eguchi S, Tomonaga T, Miyazaki K, Hamasaki K, Tokai H, Hidaka M, Yamanouchi K, Takatsuki M, Okudaira S, Tajima.Y, Kanematsu T
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Journal Title
Digestive Diseases and Sciences 54
Pages: 1597-601
NAID
Related Report
Peer Reviewed
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[Journal Article] Hepatitis C virus kinetics during the first phase of pegylated interferon-α-2b with ribavirin therapy in patients with living donor liver transplantation.2009
Author(s)
Ichikawa T, Nakao K, Miyaaki H, Eguchi S, Takatuski M, Fujimito M, Akiyama M, Miuma S, Ozawa E, Shibata H, Takeshita S, Kanematsu T, Eguchi K
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Journal Title
Hepatology Research 39
Pages: 856-864
NAID
Related Report
Peer Reviewed
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[Journal Article] Actual therapeutic efficacy of pre-transplant treatment on hepatocellular carcinoma and its impact on survival after salvage living donor liver transplantation.2009
Author(s)
Eguchi S, Hidaka M, Tomonaga T, Miyazaki K, Inokuma T, Takatsuki M, Okudaira S, Yamanouchi K, Miyaaki H, Ichikawa T, Tajima Y, Kanematsu T
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Journal Title
Journal of Gastroenterology 44
Pages: 624-629
NAID
Related Report
Peer Reviewed
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[Journal Article] The significance of enzyme immunoassay for the assessment of hepatitis B virus core-related antigen following liver transplantation.2009
Author(s)
Fujimoto M, Ichikawa T, Nakao K, Miyaaki H, Shibata H, Eguchi S, Takatsuki M, Nagaoka S, Yatsuhashi H, Kanematsu T, Eguchi K
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Journal Title
Internal medicine journal 48
Pages: 1577-1583
NAID
Related Report
Peer Reviewed
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[Journal Article] Comparison of the outcomes between an anatomical subsegme ntectomy and a non-anatomical minor hepatectomy for single hepatocellular carcinomas based on a Japanese nationwide survey.2008
Author(s)
Eguchi S. Kanematsu T, Arii S, Okazaki M, Okita K, Omata M, Ikai I, Kudo M, Kojiro M, Makuuchi M, Monden M, Matsuyama Y, Nakanuma Y, Takayasu K.
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Journal Title
Surgery 143
Pages: 469-475
Related Report
Peer Reviewed
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[Presentation] 肝移植摘出肝を用いた肝細胞癌例での肝静脈造影所見2008
Author(s)
日高匡章, 江口 晋, 高槻光寿, 山之内孝彰, 渡海大隆, 濱崎幸司, 猪熊孝実, 朝長哲生, 黒木 保, 田島義証, 兼松隆之
Organizer
第20回日本肝胆膵外科学会学術集会
Place of Presentation
山形
Related Report
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