Therapy strategy for inflammation and cancer by using the phenotype of multitargeting microRNAs.
| Project/Area Number |
20591530
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
KAMOHARA Hidenobu Kumamoto University, 医学部附属病院, 講師 (90398222)
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| Co-Investigator(Kenkyū-buntansha) |
HIROTA Masahiko 熊本大学, 医学部附属病院, 非常勤講師 (80284769)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | icroRNA / cancer / inflammation / 分子生物学 / 実験外科学 / microRNA / Cancer / Inflammation |
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| Research Abstract |
In order to analyze both cancer and inflammation associated-microRNAs, we used leukocytes before and after operation, and THP-1 cells for assay of cytokines and microRNAs expression. IL-8 mRNA expression of leukocytes was significant higher in drainage fluid than in peripheral blood. That indicated severe inflammation occurred in local surgical field. Some kind of microRNAs were detected highly in local sites. Detection of miR-21 and miR146a in a late phase suggested that could play a role of negative feedback regulator after activation of signal transduction. LPS, TNFα and IL-1β promoted the expression of miR-21 significantly in a dose dependent fashion in THP-1 cells. We determined the effect of IL-6 on the biological function in colon and pancreas cancer. IL-6 activated the phosophorylation of STAT-3 and promoted cancer progression, such as proliferation and invasion. IL-6 suppressed let7a expression and induced miR146a expression. We also identified some microRNAs which were associated with cancer and inflammation. We need to identify the phenotype of microRNAs in vivo to establish targeting therapy by using microRNAs. That will contribute to discover and apply for novel microRNA therapy against cancer and inflammation.
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Report
(4 results)
Research Products
(60 results)
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[Journal Article] Is an estimation of physiologic ability and surgical stress able to predict operative morbidity after pancreaticoduodenectomy2010
Author(s)
Hashimoto D, Takamori H, Sakamoto Y, Ikuta Y, Nakahara O, Furuhashi S, Tanaka H, Watanabe M, Beppu T, Hirota M, Baba H.
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Journal Title
J Hepatobiliary Pancreat Sci. 17(2)
Pages: 132-138
NAID
Related Report
Peer Reviewed
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[Journal Article] The role of oxysterol binding protein-related protein 5 in pancreatic cancer.2010
Author(s)
Ishikawa S, Nagai Y, Masuda T, Koga Y, Nakamura T, Imamura Y, Takamori H, Hirota M, Funakosi A, Fukushima M, Baba H.
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Journal Title
Cancer Sci. 101(4)
Pages: 898-905
NAID
Related Report
Peer Reviewed
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[Journal Article] Clinical significance of dihydropyrimidine dehydrogenase and thymidylate synthase expression in patients with pancreatic cancer.2010
Author(s)
Nakahara O, Takamori H, Tanaka H, Sakamoto Y, Ikuta Y, Furuhashi S, Watanabe M, Beppu T, Hirota M, Kanemitsu K, Baba H.
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Journal Title
Int J Clin Oncol. 15
Pages: 39-45
NAID
Related Report
Peer Reviewed
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[Journal Article] Clinical significance of dihydropyrimidine dehydrogenase and thymidylate synthase expression in patients with pancreatic cancer.2010
Author(s)
Nakahara O, Takamori H, Tanaka H, Sakamoto Y, Ikuta Y, Furuhashi S, Watanabe M, Beppu T, Hirota M, Kanemitsu K, Baba H.
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Journal Title
Int J Clin Oncol.
Volume: 15
Pages: 39-45
NAID
Related Report
Peer Reviewed
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[Journal Article] Significance of endothelial molecular markers in the evaluation of the severity of acute pancreatitis.2009
Author(s)
Ida S, Fujimura Y, Hirota M, Imamura Y, Ozaki N, Suyama K, Hashimoto D, Ohmuraya M, Tanaka H, Takamori H, Baba H.
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Journal Title
Surg Today. 39(4)
Pages: 314-319
NAID
Related Report
Peer Reviewed
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[Journal Article] MicroRNA-21 regulates the proliferation and invasion in esophageal squamous cell carcinoma.2009
Author(s)
Hiyoshi Y, Kamohara H, Karashima R, Sato N, Imamura Y, Nagai Y, Yoshida N, Toyama E, Hayashi N, Watanabe M, Baba H.
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Journal Title
Clin.Cancer Res. 15(6)
Pages: 1915-1922
Related Report
Peer Reviewed
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