| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
In order to analyze both cancer and inflammation associated-microRNAs, we used leukocytes before and after operation, and THP-1 cells for assay of cytokines and microRNAs expression. IL-8 mRNA expression of leukocytes was significant higher in drainage fluid than in peripheral blood. That indicated severe inflammation occurred in local surgical field. Some kind of microRNAs were detected highly in local sites. Detection of miR-21 and miR146a in a late phase suggested that could play a role of negative feedback regulator after activation of signal transduction. LPS, TNFα and IL-1β promoted the expression of miR-21 significantly in a dose dependent fashion in THP-1 cells. We determined the effect of IL-6 on the biological function in colon and pancreas cancer. IL-6 activated the phosophorylation of STAT-3 and promoted cancer progression, such as proliferation and invasion. IL-6 suppressed let7a expression and induced miR146a expression. We also identified some microRNAs which were associated with cancer and inflammation. We need to identify the phenotype of microRNAs in vivo to establish targeting therapy by using microRNAs. That will contribute to discover and apply for novel microRNA therapy against cancer and inflammation.
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