Introduction of humanβ-defensin-3 and human cathelicidine antimicrobial peptide-18 into cultured human skin cells

• Project/Area Number: 20591534
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Tokai University
• Principal Investigator: INOKUCHI Sadaki 東海大学, 医学部, 教授 (60160008)
• Research Collaborator: ANDO Kiyoshi 東海大学, 医学部, 教授 (70176014) ; UMEZAWA Kazuo 東海大学, 医学部, 講師 (30349344) ; SUZUKI Yousuke 東海大学, 医学部, 講師 (90459454)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 移植・再生医療 / 培養皮膚 / 抗菌ペプチド / ウイルスベクター / LL37 / hBD3 / 再生医療 / hCAP18

Research Abstract

Humanβ-defensin-3(hBD3) gene and human cathelicidine antimicrobial peptide-18(hCAP18/LL37) gene were introduced into cultured human skin cells simultaneously by a recombinant adenovirus vector. The virus infected cells secreted both hBD3 and hCAP18, a pro-peptide of LL37.

Research Products

• [Journal Article] Introduction of humanβ-defensin-3 into cultured human keratinocytes and fibroblasts by infection of a recombinant adenovirus vector (2011)
  • Author(s): Suzuki Y, Inokuchi S, Takazawa K, Umezawa K, Saito T, Kidokoro M, Tanaka M, Matsuzawa H, Inoue S, Tsuchiya I, Ando K
  • Journal Title: Burns Volume: 37(1) Pages: 109-16
  • Peer Reviewed
• [Journal Article] Introduction of human β-defensin-3 into cultured human keratinocytes and fibroblasts by infection of a recombinant adenovirus vector. (2011)
  • Author(s): Suzuki Y, Inokuchi S, Takazawa K, Umezawa K, Saito T, Kidokoro M, Tanaka M, Matsuzawa H, Inoue S, Tuchiya I, Ando K.
  • Journal Title: Burns. 37(1) Pages: 109-116
  • Peer Reviewed
• [Journal Article] Introduction of human β-defensin-3 into cultured human keratinocytes and fibroblasts by infection of a recombinant adenovirus vector (2011)
  • Author(s): Suzuki Y, Inokuchi S, Takazawa K, Umezawa K, Saito T, Kidokoro M, Tanaka M, Matsuzawa H, Inoue S, Tachiya I, Ando K.
  • Journal Title: Burns. Volume: 37(1) Pages: 109-16
  • Peer Reviewed
• [Journal Article] The effectiveness of basic fibroblast growth factor in fibrin-based cultured skin substitute in vivo (2009)
  • Author(s): Inoue S, Kijima H, Kidokoro M, Tanaka M, Suzuki Y, Motojuku M, Inokuchi S
  • Journal Title: J Burn Care Res Volume: 30(3) Pages: 514-9
  • Peer Reviewed
• [Journal Article] The effectiveness of basic fibroblast growth factor in fibrin-based cultured skin substitute in vivo. (2009)
  • Author(s): Inoue S, Kijima H, Kidokoro M, Tanaka M, Suzuki Y, Motojuku M, Inokuchi S.
  • Journal Title: J Burn Care Res. 30(3) Pages: 514-519
  • Peer Reviewed
• [Presentation] 培養皮膚移植後の血管新生に対する高気圧酸素療法の影響 (2011)
  • Author(s): 秋枝一基, 梅澤和夫, 吉井久倫, 石川祥一朗, 飯塚進一, 田中真紀子, 城所正子, 猪口貞樹
  • Organizer: 第19回日本熱傷学会関東地方会
  • Place of Presentation: スクワール麹町
  • Year and Date: 2011-01-28
• [Presentation] 熱傷治療の先端技術:バイオ人工皮膚の開発と応用 (2008)
  • Author(s): 猪口貞樹
  • Organizer: 第19回日本急性血液浄化学会
  • Place of Presentation: 御殿山ガーデンホテルラフォーレ東京
  • Year and Date: 2008-09-20
• [Presentation] 培養皮膚の抗菌性向上を目指して: human-β-defensin遺伝子組換え細胞のさらなる検討 (2008)
  • Author(s): 鈴木陽介、梅澤和夫、井上茂亮、高沢研丞、斎藤剛、田中真紀子、城所正子、猪口貞樹
  • Organizer: 第34回日本熱傷学会総会・学術集会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-06-28
• [Presentation] β-デフェンシンの抗菌活性と遺伝子組換え細胞の作製 (2008)
  • Author(s): 鈴木陽介, 井上茂亮, 斉藤剛, 梅澤和夫, 田中真紀子, 城所正子, 猪口貞樹
  • Organizer: 遺伝子発現の検討 熱傷(0285-113X)
• [Presentation] 培養皮膚の抗菌性向上を目指して human-β-defensin遺伝子組換え細胞のさらなる検討 (2008)
  • Author(s): 鈴木陽介、梅澤和夫, 井上茂亮, 高澤研丞, 斉藤剛, 田中真紀子, 城所正子, 猪口貞樹
  • Organizer: 熱傷(0285-113X)
• [Presentation] 熱傷治療の先端技術 : バイオ人工皮膚の開発と応用 (2008)
  • Author(s): 猪口貞樹
  • Organizer: 第19回日本急性血液浄化学会
  • Place of Presentation: 東京