Analysis of the mechanism of islet injury during islet transplantation using electron spin resonance.
| Project/Area Number |
20591539
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | 国立病院機構 千葉東病院 (2010)
Clinical research Center, Chiba-East National Hospital, National Hospital Organization (2008-2009) |
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Principal Investigator |
MARUYAMA Michihiro 国立病院機構 千葉東病院, 臨床研究センター, 室長 (40399754)
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| Co-Investigator(Kenkyū-buntansha) |
AKUTSU Naotake 独立行政法人・国立病院機構千葉東病院, 臨床研究センター, 室長 (00344979)
剣持 敬 独立行政法人国立病院機構(千葉東病院臨床研究センター), センター長 (50215133)
西郷 健一 独立行政法人国立病院機構(千葉東病院臨床研究センター), 部長 (60323424)
大月 和宣 独立行政法人国立病院機構(千葉東病院臨床研究センター), 室長 (50399755)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 膵島移植 / フリーラジカル / ヒドロキシラジカル / 膵島分離 / スピントラップ法 / 電子スピン共鳴 / 膵島障害 / 活性酸素 / ラットモデル |
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| Research Abstract |
Free radical adducts was easily detected by electron spin resonance (ESR) using 2-(5,5-Dimethyl-2-oxo-2λ5-[1,3,2]dioxaphosphinan-2-yl)-2-methyl-3,4 -dihydro-2H-pyrrole 1-oxide (CYPMPO). Signal of free radical adducts did not decrease by adding UW solution or ET-Kyoto solution as organ preserve solution. Oxidative stress during islet isolation may affect islet yield. It was very difficult to detect free radical adducts in blood sample of islet isolation model.
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Report
(4 results)
Research Products
(32 results)
