Basic and clinical study of new diagnostic method for gastrointestinal cancer using the ratio of free DNA fragments in the serum

• Project/Area Number: 20591558
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Fujita Health University
• Principal Investigator: OZAWA Soji Fujita Health University, 医学部・消化器外科学, 教授 (10169287)
• Co-Investigator(Renkei-kenkyūsha): SHIRAISHI Tenzou 藤田保健衛生大学, 坂文種報徳會病院一般消化器外科, 講師 (00449464) ; OOSHIMA Hisanori 藤田保健衛生大学, 坂文種報徳會病院一般消化器外科, 助教 (70367712) ; ARAKAWA Satoshi 藤田保健衛生大学, 坂文種報徳會病院一般消化器外科, 助教
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 消化器癌 / DNA断片 / 悪性度診断法 / 血清DNA / 核酸 / 腫瘍マーカー / 血行性転移

Research Abstract

The aim of this study was to analyze in the serum of nude mice that were implanted colorectal cancer cells and in the serum of colorectal cancer patients using Alu DNA sequences (Alu247 and Alu115) and to reveal whether Alu247/Alu115 ratio (Alu ratio) is related to outcome of treatments and to clinical-oncological factors. We concluded that the Alu ratio might be useful for predicting effects of chemotherapy and for discriminating between cancer and non-cancer. The Alu ratio was considered to have a higher discriminatory ability for diagnosing cancer than the CEA and CA19-9 tumor marker levels.

Research Products

• [Journal Article] Gene expression and effects of orally active derivatives off fluoropyrimidine on gastric and colorectal cancer (2010)
  • Author(s): Arakawa S, Ozawa S, Ooshima H, 他
  • Journal Title: Experimental and Therapeutic Medicine 1(1) Pages: 331-336
  • Peer Reviewed
• [Journal Article] Gene expression and effects of orally active derivatives off fluoropyrimidine on gastric and colorectal cancer. (2010)
  • Author(s): Arakawa S, et al
  • Journal Title: Experimental and Therapeutic Medicine Volume: 1 Pages: 331-336
  • Peer Reviewed
• [Journal Article] Gene expression and effects of orally active derivatives off fluoropyrimidine on gastric and colorectal cancer (2010)
  • Author(s): Arakawa S, Ozawa S, el al.
  • Journal Title: Experimental and Therapeutic Medicine 1 Pages: 331-336
  • Peer Reviewed
• [Presentation] 血清遊離DNA断片比による大腸癌高感度診断法の検討 (2010)
  • Author(s): 荒川敏、小澤壯治、大島久徳、他
  • Organizer: 第69回日本癌学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-24
• [Presentation] 血清遊離DNA断片比による消化器癌の悪性度診断の基礎的検討 (2010)
  • Author(s): 大島久徳、小澤壯治、荒川敏、他
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2010-04-08
• [Presentation] 胃癌と大腸癌における抗癌剤感受性関連遺伝子の発現と経口フッ化ピリミジン系抗癌剤の効果に関する検討 (2009)
  • Author(s): 荒川敏、小澤壯治、大島久徳、他
  • Organizer: 第68回日本癌学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-01