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Development of anti-cancer drug modified by the peptide binding to cholangiocarcinoma.

Research Project

Project/Area Number 20591588
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionShinshu University

Principal Investigator

ISHIZONE Satoshi  Shinshu University, 医学部附属病院, 助教 (90419341)

Co-Investigator(Kenkyū-buntansha) MIYAGAWA Shinichi  信州大学, 医学部, 教授 (80229806)
KOMATSU Daisuke  信州大学, 医学部附属病院, 助教 (50507481)
MURANAKA Futoshi  信州大学, 医学部附属病院, 医員 (80507492)
Research Collaborator SHIMIZU Akira  信州大学, 医学部附属病院, 助教 (00447773)
KITAHARA Hiroe  信州大学, 医学部附属病院, 医員 (20569321)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsバイオパンニング法 / 消化器癌標的治療 / ペプチド / バイオパニング法
Research Abstract

Cholangiocarcinoma (CCA) is a common carcinoma of the liver, and the majority of patients with CCA have a poor prognosis due to the lack of effective non-surgical therapies in addition to its rapid progression and inoperability at the time of diagnosis. The development of novel non-surgical therapeutics that efficiently target CCA could significantly improve the prognosis for patients presenting with CCA. Here, we describe the iteration and characterization of a novel peptide, designated COP35 (CCA-binding oligo-peptide #35) that binds selectively to human CCA identified by bacteriophage biopanning using the intrahepatic cholangiocarcinoma cell line RBE and the normal cholangiocyte cell line MMNK-1. COP35 was found to augment the growth inhibitory effects of 5-FU against RBE cells. Utilizing pull down assay and liquid chromatography, we identify the clathrin heavy chain (CHC) accompanied by GRP78/BiP as a COP35 binding partner. In summary, we identify COP35 as a possible candidate for peptide targeted therapies for CCA.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (25 results)

All 2011 2010 2009 2008

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (8 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] CoP35, a cholangiocarcinoma-binding oligo-peptide, interacts with the clathrin heavy chain accompanied by GRP78.2011

    • Author(s)
      Kitahara H, Masumoto J, Parker AL, Maruta F, Kubo N, Shimizu A, Akita N, Miwa S, Kobayashi N, Nakayama J, Miyagawa S.
    • Journal Title

      Mol Cancer Res. in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Noncompliance with medications in pediatric patients after living-donor liver transplantation.2010

    • Author(s)
      Masuda Y, Mita A, Ohno Y, Urata K, Nakazawa Y, Ikegami T, Masaru T, Miyagawa S.
    • Journal Title

      Transplant Proc. 42(10)

      Pages: 4191-4192

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Advances intherapeutics for liver metastasis from colorectal cancer.2010

    • Author(s)
      Kobayashi A, Miyagawa S.
    • Journal Title

      World J Gastrointest Oncol. 15;2(10)

      Pages: 380-389

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of portal branch ligation-stimulated Hmga2-positive bipotent hepatic progenitor cells.2010

    • Author(s)
      Sakai H, Tagawa Y, Tamai M, Motoyama H, Ogawa S, Soeda J, Nakata T, Miyagawa S.
    • Journal Title

      Biochem Biophys Res Commun. 17;403(3-4)

      Pages: 298-304

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of high endothelial venule-like vessels expressing GIcNAc6ST-1-mediated L-selectin ligand carbohydrate and mucosal addressin cell adhesion molecule 1 (MAdCAM-1) in a mouse model of "Candidatus Helicobacter heilmannii"-induced gastritis and gastric mucosa-associated lymphoid tissue (MALT) lymphoma.2010

    • Author(s)
      Suzuki A, Kobayashi M, Matsuda K, Matsumoto T, Kawakubo M, Kumazawa S, Koide N, Miyagawa S, Ota H.
    • Journal Title

      Helicobacter. 15(6)

      Pages: 538-548

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antiproinflammatory effects of iodixanol (OptiPrep)-based density gradient purification on human islet preparations.2010

    • Author(s)
      Mita A, Ricordi C, Messinger S, Miki A, Misawa R, Barker S, Molano RD, Haertter R, Khan A, Miyagawa S, Pileggi A, Inverardi L, Alejandro R, Hering BJ, Ichii H.
    • Journal Title

      Cell Transplant. 19(12)

      Pages: 1537-1546

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Esophageal cancer associated with other primary cancers--historical comparison of clinicopathologic features in 359 esophageal cancer patients.2010

    • Author(s)
      Koide N, Komatsu D, Hiraga R, Kitazawa M, Suzuki A, Miyagawa S.
    • Journal Title

      Hepatogastroenterology. 57(99-100)

      Pages: 513-518

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of complement regulatory protein on porcine endogenous retrovirus (PERV) depends on molecular size.2010

    • Author(s)
      Yamamoto A, Kobayashi C, Yamashita S, Miyazawa T, Okabe M, Fukuzawa M, Miyagawa S.
    • Journal Title

      Transpl Immunol. 23(1-2)

      Pages: 71-76

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adrenomedullin in sinusoidal endothelial cells play protective roles against cold injury of liver.2010

    • Author(s)
      Iinuma N, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Arai T, Yoshizawa T, Koyama T, Uetake R, Kawate H, Muto S, Tagawa Y, Miyagawa S, Shindo T.
    • Journal Title

      Peptides. 31(5)

      Pages: 865-871

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Disease recurrence patterns after RO resection of hilar cholangiocarcinoma.2010

    • Author(s)
      Kobayashi A, Miwa S, Nakata T, Miyagawa S.
    • Journal Title

      Br J Surg. 97(1)

      Pages: 56-64

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potentials of regenerative medicine for liver disease.2009

    • Author(s)
      Ogawa S, Miyagawa S.
    • Journal Title

      Surg Today. 39(12)

      Pages: 1019-1025

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Administration of dalteparin based on the activated clotting time for prophylaxis of hepatic vessel thrombosis in living donor liver transplantation.2009

    • Author(s)
      Uchikawa Y, Ikegami T, Masuda Y, Ohno Y, Mita A, Urata K, Nakazawa Y, Terada M, Miyagawa S.
    • Journal Title

      Transplant Proc. 41(9)

      Pages: 3784-3790

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro reprogramming of adult hepatocytes into insulin-producing cells without viral vectors.2009

    • Author(s)
      Motoyama H, Ogawa S, Kubo A, Miwa S, Nakayama J, Tagawa Y, Miyagawa S.
    • Journal Title

      Biochem Biophys Res Commun. 17;385(1)

      Pages: 123-128

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential feasibility of early bone marrow cell injection into the spleen for creating functional hepatocytes.2009

    • Author(s)
      Misawa R, Soeda J, Ise H, Takahashi M, Kubota K, Mita A, Nakata T, Miyagawa S.
    • Journal Title

      Transplantation. 27;87(8)

      Pages: 1147-1154

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinical and pathological features of primary carcinoma of the cystic duct2009

    • Author(s)
      Nakata T, Kobayashi A, Miwa S, Soeda J, Uehara T, Miyagawa S.
    • Journal Title

      J Hepatobiliary Pancreat Surg 16(1)

      Pages: 75-82

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of oligopeptide to colon cancer cells separated from patients using laser capture microdissection2008

    • Author(s)
      Naoli Kubo, et al
    • Journal Title

      Journal of Drug Targeting 16

      Pages: 396-404

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] バイオパンニング法による胆管癌特異的ペプチドの同定2010

    • Author(s)
      北原弘恵
    • Organizer
      第52回日本消化器病学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-10-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] バイオパンニング法による胆管癌特異的ペプチドの同定2010

    • Author(s)
      北原弘恵、増本純也、清水明、久保直樹、秋田倫幸、三輪史郎、中山淳、宮川眞一
    • Organizer
      第52回日本消化器病学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2010-10-13
    • Related Report
      2010 Final Research Report
  • [Presentation] バイオパンニング法による胆管癌特異的ペプチドの同定2010

    • Author(s)
      北原弘恵、増本純也、久保直樹、清水明、秋田倫幸、三輪史郎、中山淳、宮川眞一
    • Organizer
      第110回日本外科学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2010-04-08
    • Related Report
      2010 Final Research Report
  • [Presentation] バイオパンニング法による胆管癌特異的ペプチドの同定2009

    • Author(s)
      北原弘恵、久保直樹、清水明、秋田倫幸、三輪史郎、宮川眞一
    • Organizer
      第108回日本外科学会総会
    • Place of Presentation
      長崎市
    • Year and Date
      2009-05-15
    • Related Report
      2010 Final Research Report
  • [Presentation] バイオパンニング法による胆管癌特異的ペプチドの同定2009

    • Author(s)
      北原弘恵
    • Organizer
      第109回日本外科学会定期学術総会
    • Place of Presentation
      福岡市
    • Related Report
      2009 Annual Research Report
  • [Presentation] バイオパンニング法による胆管癌特異的ペプチドの同定2009

    • Author(s)
      北原弘恵
    • Organizer
      第47回日本癌治療学会総会
    • Place of Presentation
      横浜市
    • Related Report
      2009 Annual Research Report
  • [Presentation] バイオパンニング法による月旦管癌特異的ペプチドの同定2008

    • Author(s)
      北原弘恵、丸田福門、久保直樹、清水明、秋田倫幸、三輪史郎、宮川眞一
    • Organizer
      第46回日本癌治療学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2008-10-30
    • Related Report
      2010 Final Research Report
  • [Presentation] バイオパンニング法による胆管癌特異的結合ケプチドの同定2008

    • Author(s)
      北原弘恵
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-28
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] クリアランス結合性ペプチド誘導体2009

    • Inventor(s)
      宮川眞一、清水明、北原弘恵、増本純也
    • Industrial Property Rights Holder
      信州大学
    • Industrial Property Number
      2009-250552
    • Filing Date
      2009-10-30
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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