| Project/Area Number |
20591601
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Chiba University |
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Principal Investigator |
TAKEUCHI Dan Chiba University, 医学部附属病院, 助教 (20400822)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Masaru 千葉大学, 大学院・医学研究院, 教授 (70166156)
YOSHIDOME Hiroyuki 千葉大学, 大学院・医学研究院, 講師 (10312935)
OHTSUKA Masayuki 千葉大学, 大学院・医学研究院, 講師 (90334185)
YOSHITOMI Hideyuki 千葉大学, 医学部附属病院, 助教 (60375631)
FURUKAWA Katsunori 千葉大学, 医学部附属病院, 助教 (00400987)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 肝臓外科学 / 感染症 / 移植・再生医療 / 免疫学 / 外科 / シグナル伝達 |
|---|
| Research Abstract |
In rat model, 90% hepatectomy caused neutrophil dependent liver tissue injury, impaired liver regeneration, resulting in poor survival. This was accompanied by TGF-β production by HSC and suppressed expression of MRP-2 by hepatocytes. Liver tissue injury was attenuated after 70% hepatectomy. Kuppfer cells from remnant liver produced HGF and EGF. Humoral agents produced by cells in sinusoidal space were supposed to be important for liver regeneration.
In patients developing infectious complications after hepatectomy, neutrophils produced IL-1β, TNFα, and CCL2 3 days after surgery, and CD14^+ monocytes showed properties of M2 macrophages. In patients developing severe SIRS, preoperative neutrophils produced IL-1β and TNFα.
In murine model, administration of steroids caused impaired recruitment of inflammatory cells to the site of viral infection after hepatectomy. This also caused impaired production of α-defensin by neutrophils resulting in prolonged viral infection.
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