| Project/Area Number |
20591617
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
|
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
UYAMA Naoki Hyogo College of Medicine, 医学部, 助教 (70402873)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Jiro 兵庫医科大学, 医学部, 教授 (90199373)
IIMURO Yuji 兵庫医科大学, 医学部, 准教授 (30252018)
TADAMICHI Hirano 兵庫医科大学, 医学部, 講師 (90340968)
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| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 肝内胆管癌 / 間質 / 線維芽細胞 / コラーゲン / 相互作用 / 受容体 |
|---|
| Research Abstract |
Immunohistochemical study revealed that collagens type I, III, V and XII were expressed by fibroblasts in cholangiocarcinoma and collagens type V, IX, XII, XIV and XVI were expressed by cancer cells of cholangiocarcinoma. In addition, Immunohistochemical study revealed that DDR1 and Integrin beta1 receptor, which are collagen receptor, and focal adhesion kinase (FAK), an integrin downstrem molecule, were expressed by cancer cells as well as fibroblasts in cholangiocarcinoma tissues. They were also expressed by cholangiocarcinoma cell lines and fibroblasts isolated from cholangiocarcinoma tissues. In culture experiments, FAK specific inhibitor suppressed cell growth and cell invaison ability of RBE cells, SSP-25 cells, cell lines of cholangiocarcinoma, and fibroblasts. Taken together, FAK may be an important molecule to regulate matrix-FAK signaling dependant cancer behavors.
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