Screening for carcinogenesis-related proteins and diagnostic markers of pancreatic cancer by proteomic analysis

Research Project

Project/Area Number	20591628
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Digestive surgery
Research Institution	Yamaguchi University
Principal Investigator	YOSHINO Shigefumi Yamaguchi University, 医学部附属病院, 准教授 (60294633)
Co-Investigator(Kenkyū-buntansha)	TAMESA Akuo 山口大学, 医学部附属病院, 助教 (30359905) SAKAMOTO Kazuhiko 山口大学, 医学部附属病院, 助教 (50420526)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	膵癌 / プロテオーム解析 / 発癌関連蛋白質 / 早期診断マーカー / 血清マーカー / α-1-antitripsin
Research Abstract	Pancreatic cancer (PC) is one of the most lethal malignant tumors because of late diagnosis and the lack of response to various therapies. To identify potential biomarkers in cancerous serum from early stage PC patients, we carried out two-dimensional gel electrophoresis (2-DE) to compare the serum proteomic profiles from 45 patients with PC and 20 healthy volunteers. Two spots showed differential expression on 2-DE gels and these up-regulated protein spots were identified as α-1-antitrypsin (AAT). These protein spots were also confirmed by Western blotting. The serum isoforms of AAT might be clinically useful for PC diagnosis and monitoring.