Identification of microRNA which is related to chemoresistance of pancreatic cancer, and intensification of the sensitivity by controlling the microRNA.

• Project/Area Number: 20591629
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: TOMA Hiroki Kyushu University, 大学病院, 助教 (80437780)
• Co-Investigator(Kenkyū-buntansha): MIZUMOTO Kazuhiro 九州大学, 大学病院, 准教授 (90253418) ; OHUCHIDA Kenoki 九州大学, 医学研究院, 客員助教 (20452708)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵臓外科学 / 膵癌 / microRNA / 治療抵抗性 / miRNA / 膵液 / Gemcitabine / マイクロダイセクション

Research Abstract

We established two Gemcitabine-resistant pancreatic cancer cell lines, and global miRNA expression analyses was performed.24 miRNA were candidates that were related to chemoresistance of pancreatic cancer. MiR142-5p and miR204 show most highest expression in two Gemcitabine-resistant pancreatic cancer cell lines. MiR17-5p, miR200c and miR203 were poor prognosis factors. MiR21 and miR155 in pancreatic juice have the potential of becoming new biomarkers for diagnosing pancreatic cancer. MiR21 modulates biological functions of pancreatic cancer cells including their proliferation, invasion, and chemoresistance.

Research Products

• [Journal Article] MicroRNA Expression as a Predictive Marker for Gemcitabine Response after Surgical Resection of Pancreatic Cancer. (2011)
  • Author(s): Ohuchida K, et al.
  • Journal Title: Ann Surg Oncol. 118 Pages: 405-411
• [Journal Article] MicroRNA expression analyses in preoperative pancreatic juice samples of pancreatic ductal adenocarcinoma. (2010)
  • Author(s): Sadakari Y., et al.
  • Journal Title: JOP 11(6) Pages: 587-92
• [Journal Article] MicroRNA miR-17-5p is overexpressed in pancreatic cancer, associated with a poor prognosis, and involved in cancer cell proliferation and invasion. (2010)
  • Author(s): Yu J., et al.
  • Journal Title: Cancer Biol Ther. 10(8) Pages: 748-57
• [Journal Article] MicroRNA-203 expression as a new prognostic marker of pancreatic adenocarcinoma. (2010)
  • Author(s): Ikenaga N., et al.
  • Journal Title: Ann Surg Oncol. 17(12) Pages: 3120-3128
• [Journal Article] MicroRNA (2010)
  • Author(s): Yu J., et al.
  • Journal Title: hsa-miR-200c, is an independent prognostic factor in pancreatic cancer and its upregulation inhibits pancreatic cancer invasion but increases cell proliferation Mol Cancer. 28;9 Pages: 169-169
• [Journal Article] MicroRNA-21 modulates biological functions of pancreatic cancer cells including their proliferation, invasion, and chemoresistance. (2009)
  • Author(s): Moriyama T., et al.
  • Journal Title: Mol Cancer Ther 8(5) Pages: 1067-1074
• [Journal Article] MicroRNA-21 modulates biological functions of pancreatic cancer cells in cluding their proliferation, invasion, and chemoresistance (2009)
  • Author(s): 森山大樹
  • Journal Title: Molecular Cancer Therapy 8(5) Pages: 1067-1074
  • Peer Reviewed
• [Presentation] Joint Meeting of the International Association of Pancreatology and the Japan Pancreas Society 2010, Japan (2010)
  • Author(s): Ikenaga N
  • Organizer: Joint Meeting of the International Association of Pancreatology and the Japan Pancreas Society
  • Place of Presentation: Japan
• [Presentation] MicroRNA-21 Modulates Biologic Functions of Pancreatic Cancer Cells Including Their Proliferation (2010)
  • Author(s): Moriyama T
  • Organizer: Invation and Chemoresistance, International Association of Pancreatology and the Japan Pancreas Society
  • Place of Presentation: Japan
• [Presentation] 網羅的発現解析による膵癌の治療抵抗性に関わるmicroRNAの同定 (2009)
  • Author(s): 大内田研宙
  • Organizer: 第109回 日本外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-04-02
• [Presentation] MicroRNA-200c is a novel independent prognostic marker for pancreatic cancer. (2009)
  • Author(s): Yu J
  • Organizer: 40th Anniversary Meeting of the American Pancreatic Association and Japan Pancreas Association
  • Place of Presentation: United States of America
• [Remarks] ホームページ等 なし