Participation of Wee1 gene to carcinogenesis of the pancreatic cancer in new genetically-modified mouse and clinical implications of Wee1 gene

• Project/Area Number: 20591630
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: TOMINAGA Yohei Kyushu University, 医学研究院, 特任講師 (90304823)
• Co-Investigator(Kenkyū-buntansha): NAGAI Eishi 九州大学, 医学研究院, 准教授 (30264021) ; OHUCHIDA Kenoki 九州大学, 医学研究院, 客員助教 (20452708)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵臓外科学 / 膵癌 / 細胞周期 / microdissection / Weel / G2 / Mチェツクポイント / ノックアウトマウス / 膵臓がん / Wee1 / Mチェックポイント

Research Abstract

WEE1 mRNA expression in pancreatic cancer cell lines is higher than that in HPDE cells. Six normal pancreas tissues and 51 PDAC samples were analyzed by immunostaining. We did not find any significant association between WEE1 expression and various clinicopathologic factors in patients with pancreatic caner. There was no difference in overall survival and disease free survival between WEE1-positive and WEE1-negtive groups. Unfortunately, we did not recognize the clinical implications of Wee1 gene to the pancreatic cancer.

Research Products

• [Journal Article] Combination with low-close gemcitabine and hTERT-promoter-dependent conditionally replicative adenovirus enhances cytotoxicity through their crosstalk mechanisms in pancreatic cancer (2010)
  • Author(s): Nagai E(3番目)
  • Journal Title: Cancer Lett
  • Peer Reviewed
• [Journal Article] S100P is a novel marker to identify intraductal papillary mucinous neoplasms (2010)
  • Author(s): Nagai E(2番目)
  • Journal Title: Hum Pathol.
  • Peer Reviewed
• [Journal Article] CD44v6 expression in intraductal papillary mucinous neoplasms of the pancreas (2010)
  • Author(s): Nagai E(2番目)
  • Journal Title: Pancreas 39 Pages: 31-35
  • Peer Reviewed
• [Journal Article] hTERT-promoter-dependent oncolytic adenovirus enhances the transduction and therapeutic efficacy of replication-defective adenovirus vectors in pancreatic cancer cells (2009)
  • Author(s): Nagai E(4番目)
  • Journal Title: Cancer Sci. 101 Pages: 735-742
  • Peer Reviewed
• [Journal Article] S100A4 mRNA is a diagnostic and prognostic marker in pancreatic carcinoma (2009)
  • Author(s): Nagai E(9番目)
  • Journal Title: J Gastrointest Surg. 13 Pages: 1852-1858
  • Peer Reviewed
• [Journal Article] LMO2 is a novel predictive marker for a better prognosis in pancreatic cancer (2009)
  • Author(s): Nagai E(3番目)
  • Journal Title: Neoplasia 11 Pages: 712-719
  • Peer Reviewed
• [Journal Article] MicroRNA-21 modulates biological functions of pancreatic cancer cells including their proliferation, invasion, and chemoresistance (2009)
  • Author(s): Nagai E(9番目)
  • Journal Title: Mol Cancer Ther. 8 Pages: 1067-1074
  • Peer Reviewed
• [Journal Article] Antagonistic Regulation of Cell-Matrix Adhesion by FosB and deltaFosB/delta2deltaFosB Encoded by Alternatively Spliced Forms of fosB Transcripts (2008)
  • Author(s): Olinishi Y, Tominaga Y(6番目)
  • Journal Title: Molecular Biology of the Cell 19 Pages: 4717-4729
  • Peer Reviewed
• [Presentation] Murine Weel plays a critical role in cell cycle regulation and pre-implanatation stages of embryonic development (2008)
  • Author(s): 冨永 洋平
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Remarks] ホームページ等