Pre-clinical study on the protective effect of drug inhalation on the lung graft from donor after cardiac death

• Project/Area Number: 20591668
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: BANDO Toru Kyoto University, 大学院・医学研究科, 准教授 (20293954)
• Co-Investigator(Kenkyū-buntansha): DATE Hiroshi 京都大学, 大学院・医学研究科, 教授 (60252962) ; SHOJI Tsuyoshi 京都大学, 大学院・医学研究科, 助教 (80402840) ; FUJINAGA Takuji 京都大学, 大学院・医学研究科, 助教 (00444456) ; CHEN Fengshi 京都大学, 大学院・医学研究科, 助教 (00452334)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 外科 / 移植・再生医療 / トランスレーショナルリサーチ

Research Abstract

We evaluated post-transplant pulmonary function of the lung graft that received 4-hour warm ischemia.in a canine left lung transplantation model. One hour mechanical ventilation with 100% oxygen during the last hour of 4-hour warm ischemia provided significantly better lung function after reperfusion than no ventilation. In the next experiment where we extended the warm ischemic time to 5 hours, the last one-hour ventilation with inhalation of a beta2-adrenoreceptor agonist (Procatelol) significantly enhanced the posttransplant lung performance than the one-hour ventilation only. We demonstrated the efficacy and safety of the combined therapy with inhalation of a beta2-agonist and mechanical ventilation for the lungs from donor after cardiac death.

Research Products

• [Journal Article] Establishment of an ex-vivo lung perfusion model using non-heart-beating large pigs (2010)
  • Author(s): Okamoto T, Chen F, Zhang J, Yamada T, Nakayama E, Morikawa H, Bando T, Date H
  • Journal Title: Transplant Proc 42 Pages: 1598-1601
  • Peer Reviewed
• [Journal Article] Beneficial effects of perioperative low-dose inhaled carbon monoxide on pulmonary allograft survival in MHC-inbred CLAWN miniature swine (2010)
  • Author(s): Sahara H, Shimizu A, Setoyama K, Oku M, Okumi M, Nishihara H, Oriyanhan W, Tasaki M, Scalea J, Wada H, Bando T, Date H, Yamada K
  • Journal Title: Transplantation 90 Pages: 1336-1343
  • Peer Reviewed
• [Journal Article] Forced oscillation technique as a noninvasive assessment for lung transplant recipients (2010)
  • Author(s): Hamakawa H, Bando T, (9番目), Date H
  • Journal Title: Adv Exp Med Biol Volume: 662 Pages: 293-298
  • Peer Reviewed
• [Journal Article] Establishment of an ex-vivo lung perfusion model using non-heart-beating large pigs (2010)
  • Author(s): Okamoto T, Bando T, (6番目), Date H
  • Journal Title: Transplant Proc Volume: 42 Pages: 1598-1601
  • Peer Reviewed
• [Journal Article] Beneficial effects of perioperative low-dose inhaled carbon monoxide on pulmonary allograft survival in MHC-inbred CLAWN miniature swine (2010)
  • Author(s): Sahara H, Bando T, (10番目), Date H, Yamada K
  • Journal Title: Transplantation Volume: 90 Pages: 1336-1343
  • Peer Reviewed
• [Journal Article] Nebulized phosphodiesterase III inhibitor during warm ischemia attenuates pulmonary ischemia-reperfusion injury (2009)
  • Author(s): Zhang J, Chen F, Zhao X, Aoyama A, Okamoto T, Fujinaga T, Shoji T, Sakai H, Cui Y, Bando T, Date H
  • Journal Title: J Heart Lung Transplant 28 Pages: 79-84
  • Peer Reviewed
• [Journal Article] Postischemic infusion of atrial natriuremic peptide attenuates warm ischemia-reperfusion injury in rut lungs (2009)
  • Author(s): Aoyama A, Chen F, Fujinaga T, Sato A, Tsuruyama T, Zhang J, Shoji T, Sakai H, Nakamura T, Date H, Wada H, Bando T
  • Journal Title: J Heart Lung Transplant 28 Pages: 628-634
  • Peer Reviewed
• [Journal Article] Protective effects of thioredoxin perfusion not inhalation in warm ischemic-reperfused rat lungs (2009)
  • Author(s): Zhang J, Chen F, Nakamura T, Fujinaga T, Aoyama A, Hamakawa H, Sakai H, Hoshino Y, Yodoi J, Wada H, Bando T, Nakamura H
  • Journal Title: Redox Rep 14 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Postischemic infusion of atrial natriuremic peptide sttenuates warm ischemia-reperfusion injury in rat lungs (2009)
  • Author(s): Aoyama A, Chen F, Fujinaga T, Sato A, Tsuruyama T, Zhang J, Shoji T, Sakai H, Nakamura T, Date H, Wada H, Bando T
  • Journal Title: J Heart Lung Transplant 28 Pages: 628-634
  • Peer Reviewed
• [Journal Article] Protective effects of thioredoxin perfusion but not inhalation in warm is chemic-reperfused rat lungs (2009)
  • Author(s): Zhang J, Chen F, Nakamura T, Fujinaga T, Aoyama A, Hamakawa H, Sakai H, Hoshino Y, Yodoi J, Wada H, Bando T, Nakamura H
  • Journal Title: Rdeox Rep 14 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Nebulized phosphodiesterase III inhibitor during warm ischemia attenuates pulmonary ischemia-reoerfusion injury (2009)
  • Author(s): Zhang J, Chen F, Zhao X, Aoyama A, Okamoto T, Fujinaga T, Shoji T, Sakai H, Cui Y, Bando T, Date H
  • Journal Title: J Heart Lung Transplant 28 Pages: 79-84
  • Peer Reviewed
• [Journal Article] Postischemic infusion of atrial natriuretic peptide attenuates warm ischemia-reperfusion injury in rat lungs (2009)
  • Author(s): Aoyama A, Chen F, Fujinaga T, Sato A, Tsuruyama T, Zhang J, Shoji T, Sakai H, Nakamura T, Date H, Wada H, Bando T
  • Journal Title: J Heart Lung Transplant
  • Peer Reviewed
• [Journal Article] Successful subzero nonfreezing preservation of rat lungs at ミ2C utilizing a new supercooling technology (2008)
  • Author(s): Okamoto T, Nakamura T, Zhang J, Aoyama A, Chen F, Fujinaga T, Shoji T, Hamakawa H, Sakai H, Manabe T, Wada H, Date H, Bando T
  • Journal Title: J Heart Lung Transplant 27 Pages: 1150-1157
  • Peer Reviewed
• [Presentation] Protective effect of ventilation before organ procurement on graft lungs donated after cardiac death in a canine lung transplantation model (2011)
  • Author(s): Sakamoto J, Yamada T, Chen F, Nakajima D, Ohsumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H,
  • Organizer: The 31st annual meeting and scientific sessions, The International Society for Heart and Lung Transplantation
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-04-14
• [Presentation] 肺移植における肺保存の現状と新たな肺保存法の検討 (2010)
  • Author(s): 藤永卓司、板東徹, (7番目)伊達洋至
  • Organizer: 第37回日本臓器保存生物医学会学術集会、シンポジウム
  • Place of Presentation: 新潟グランドホテル、新潟(招待講演)
  • Year and Date: 2010-11-20
• [Presentation] 中動物肺移植モデルを用いた摘出前換気による心停止ドナー肺の保護効果 (2010)
  • Author(s): 阪本仁、陳豊史、中島大輔、菊地柳太郎、小島史嗣、大角明宏、高橋鮎子、里田直樹、藤永卓司、庄司剛、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第63回日本胸部外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-25
• [Presentation] 中動物肺移植モデルを用いた摘出前換気による心停止ドナー肺の保護効果 (2010)
  • Author(s): 阪本仁、板東徹, (11番目)、伊達洋至
  • Organizer: 第63回日本胸部外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場、大阪(招待講演)
  • Year and Date: 2010-10-25
• [Presentation] イヌ心停止ドナー肺移植モデルにおける臓器摘出前の換気によるグラフト肺保護効果 (2010)
  • Author(s): 阪本仁、山田徹、陳豊史、中島大輔、高橋鮎子、里田直樹、藤永卓司、庄司剛、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第46回日本移植学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-22
• [Presentation] イヌ心停止ドナー肺移植モデルにおける臓器摘出前の換気によるグラフト肺保護効果 (2010)
  • Author(s): 阪本仁、板東徹, (9番目), 伊達洋至
  • Organizer: 第46回日本移植学会総会
  • Place of Presentation: 京都市勧業館みやこめっせ、京都(招待講演)
  • Year and Date: 2010-10-22
• [Presentation] Injury of donor lungs from donation after cardiac death in various settings (2010)
  • Author(s): Yamada T, Chen F, Sakamoto J, Nakajima D, Satoda N, Takahashi A, Shoji T, Fujinaga T, Sakai H, Bando T, Date H
  • Organizer: The 30th annual meeting and scientific sessions, The International Society for Heart and Lung Transplantation
  • Place of Presentation: Chicago, USA
  • Year and Date: 2010-04-23
• [Presentation] Injury of donor lungs from donation after cardiac death in various settings ; investigation by RT-PCR and pathology. The 30^<th> annual meeting and scientific sessions (2010)
  • Author(s): Yamada T, Bando T, (9番目), Date H
  • Organizer: The Intertional Society for Heart and Lung Transplantation
  • Place of Presentation: Hilton Chicago, Chicago, USA(招待講演)
  • Year and Date: 2010-04-23
• [Presentation] 心臓死ドナー肺における心停止導入条件と肺障害 (2009)
  • Author(s): 山田徹、陳豊史、阪本仁、中島大輔、里田直樹、高橋鮎子、岩切章太郎、園部誠、藤永卓司、庄司剛、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第62回日本胸部外科学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-12
• [Presentation] 心臓死ドナー肺における心停止導入条件と肺傷害 RT・PCRと病理学的検討 (2009)
  • Author(s): 山田徹、陳豊史、阪本仁、中島大輔、里田直樹、高橋鮎子、岩切章太郎、園部誠、藤永卓司、庄司剛、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第62回日本胸部外科学会定期学術集会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2009-10-12
• [Presentation] マージナルドナー肺、心臓死ドナー肺の新しい評価法と保護法 (2009)
  • Author(s): 陳豊史、岡本俊宏、山田徹、張吉天、青山晃博、藤永卓司、森川洋匡、高橋鮎子、里田直樹、庄司剛、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第45回日本移植学会総会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-09-18
• [Presentation] マージナルドナー肺、心臓死ドナー肺の新しい評価と保護法 (2009)
  • Author(s): 陳豊史、岡本俊宏、山田徹、張吉天、青山晃博、藤永卓司、森川洋匡、高橋鮎子、里田直樹、庄司剛、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第45回日本移植学会総会、シンポジウム
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2009-09-18
• [Presentation] How the cause of death affects the quality of DCD donor lungs? (2009)
  • Author(s): Yamada T, Chen F, Zhang J, Okamoto T, Fujinaga T, Morikawa H, Matsumoto I, Satoda N, Takahashi A, Shoji T, Sakai H, Bando T, Date H
  • Organizer: The 29th annual meeting and scientific sessions, The International Society for Heart and Lung Transplantation
  • Place of Presentation: Paris, France
  • Year and Date: 2009-04-23
• [Presentation] How the cause of death affects the quality of DCD donor lung? -An experimental study (2009)
  • Author(s): Yamada T, Chen F, Zhang J, Okamoto T, Fujinaga T, Morikawa H, Matsumoto I, Satoda N, Takahashi A, Shoji T, Sakai H, Bando T, Date H,
  • Organizer: The 29^<th> annual meeting and scientific sessions, The International Society for Heart and Lung Transnlantation
  • Place of Presentation: Palais des Congres, Paris, France
  • Year and Date: 2009-04-23
• [Presentation] わが国のDCDからの肺移植実現に向けての実験的取り組み (2008)
  • Author(s): 板東徹、陳豊史、藤永卓司、張吉天、青山晃博、岡本俊宏、趙向東、高橋鮎子、里田直樹、山田徹、森川洋匡、庄司剛、浜川博司、阪井宏彰、松本いずみ、伊達洋至、
  • Organizer: 第44回日本移植学会総会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-20
• [Presentation] わが国のDCDからの肺移植実現に向けての実験的取り組み (2008)
  • Author(s): 板東 徹, 他
  • Organizer: 第44回日本移植学会総会、シンポジウム
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2008-09-20
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