Biochemical and proteomic analysis of epithelial lining fluid for early diagnosis of respiratory diseases
| Project/Area Number |
20591677
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Keio University |
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Principal Investigator |
WATANABE Masazumi Keio University, 医学部, 教授 (90201227)
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| Co-Investigator(Kenkyū-buntansha) |
KOHNO Mitsutomo 慶應義塾大学, 医学部, 助教 (10276272)
HASEGAWA Naoki 慶應義塾大学, 医学部, 准教授 (20198724)
KAMIYA Kazunori 慶應義塾大学, 医学部, 助教 (80365270)
ISHIZAKA Akitoshi 慶應義塾大学, 医学部, 教授 (90176181)
KOBAYASHI Koichi 慶應義塾大学, 医学部, 名誉教授 (80051704)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 呼吸器外科 / 気道上皮被覆液 / 気管支鏡 / マイクロサンプリング法 / プロテオーム解析 / 腫瘍マーカー / 肺癌 / 非結核性抗酸菌症 / プロテオーム / マイクロサンプリング |
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| Research Abstract |
Bronchoscopic microsampling method (BMS) is a unique procedure using a newly developed probe. Bronchial epithelial lining fluid (ELF) was obtained using this method and biochemical and proteomic analyses were performed in various lung diseases. BMS was effective for the diagnosis and the prediction of therapeutic effect of lung cancer and non-tuberculous mycobacterial lung disease. 35 proteins as a candidate of tumor marker were extracted from ELF of both lung adenocarcinoma and squamous cell carcinoma patients. Measurement of cytokines and proteomic analysis could be performed in rat lung injury model. BMS has some advantages compared to conventional broncho-alveolar lavage method, because it is less invasive and can obtain highly-concentrated ELF.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Interstitial lung disease induced by gefitinib and Toll-like receptor ligands is mediated by Fra-1.2011
Author(s)
Takada Y, Gresh L, Bozec A, Ikeda E, Kamiya K, Watanabe M, Kobayashi K, Asano K, Toyama Y, Wagner EF, Matsuo K.
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Journal Title
Related Report
Peer Reviewed
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