| Project/Area Number |
20591680
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | University of Occupational and Environmental Health, Japan |
|---|
Principal Investigator |
MIZUKAMI Makiko University of Occupational and Environmental Health, Japan, 医学部, 非常勤助教 (10441859)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HANAGIRI Takeshi 産業医科大学, 医学部, 准教授 (30299614)
SHIGEMATSU Yoshiki 産業医科大学, 医学部, 助教 (10546469)
KAWAHARA Hiroharu 北九州工業高等専門学校, 細胞工学センター, 教授 (20321515)
YASUMOTO Kosei 産業医科大学, 医学部, 名誉教授 (30150452)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 抗体療法 / 肺癌 / 腫瘍抗原 / 腫瘍浸潤B細胞 / モノクローナル抗体 |
|---|
| Research Abstract |
The purpose of this study is to establish the specific antibody therapy targeting cancer antigen "UOEH-LC-1". The recombinant protein of UOEH-LC-1 was manufactured and purified. The B lymphocytes was obtained from mice spleen cells by immunization of UOEH-LC-1 protein. The hybridoma, which produced IgG antibody against UOEH-LC-1, was established by fusion of the B lymphocyte and myeloma cell. As a experimental animal model, the lung cancer cell line A110L was transplanted to SCID mice. Then, the purified monoclonal antibody was repeatedly injected from tail vein. However, the anti-tumor effect was not observed as compared to control mice.
|
