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Lipid peroxidation and PAFacetylhydrolase activity of cerebrospinal fluid in patients with subarachnoid hemorrhage

Research Project

Project/Area Number 20591696
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionTeikyo University

Principal Investigator

HIRASHIMA Yutaka  Teikyo University, 薬学部, 教授 (30135016)

Co-Investigator(Kenkyū-buntansha) DOSHI Masaru  帝京大学, 薬学部, 助教 (30392385)
YOSHIDA Yasukazu  独立行政法人産業技術総合研究所, 研究チーム長 (90358333)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords脳血管攣縮 / くも膜下出血 / 脂質過酸化 / 脂質 / 酵素 / 脳神経疾患
Research Abstract

We attempt to compare plasma PAF-AH activity and free forms of bio-marker of lipid peroxidation in cerebrospinal fluid (CSF) between patients with and without symptomatic vasospasm (SVS) after SAH. The identification of PAF-AH in CSF was performed by Western blotting. The genotype at position 279 of the plasma PAF-AH gene was determined. The activities of PAF-AH and the levels of free 8-iso-PGF_<2α> (free isoPs), free hydroxyoctadecadienoic acid (free HODE), and free hydroxyeicosatetraenoic acid (free HETE) in CSF were measured. The PAF-AH in CSF was confirmed to be only the plasma type. The genotype of the plasma PAF-AH was not different between patients with and without SVS. Free isoPs, free HODE, and free HETE showed higher values in patients without SVS in 0-4 days and 5-9 days after SAH. The PAF-AH activity also showed higher in patients without SVS in 0-4 days and 5-9 days after SAH. The associations between PAF-AH activity and free isoPs, and between PAF-AH activity and free HODE were significant.Oxidized lipids of lipoproteins and blood cell membranes produced by reactive oxygen species in CSF when SAH occurs may be the main source of lipid peroxidation. Plasma PAF-AH can hydrolize oxidized phospholipids, and may attenuate the spreading of lipid peroxidation and participate in defense mechanism against vasospasm after SAH

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (5 results)

All 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Hyperthermia during Ischemia Protects against Neuronal Death but Not Acute Brain Edema following Transient Forebrain Ischemia in Mice2009

    • Author(s)
      Doshi M
    • Journal Title

      Biol. Pharm. Bull. 32

      Pages: 1957-1961

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新人薬剤師の不安の構造と自己教育力との関係2009

    • Author(s)
      平島豊
    • Journal Title

      YAKUGAKU ZASSHI 129

      Pages: 549-556

    • NAID

      130000136158

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ヒトII型PAFアセチルヒドロラーゼ遺伝子導入マウスによる神経細胞の長期的な抗脂質過酸化効果の検討.2010

    • Author(s)
      道志勝、平島豊
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] 脳血管攣縮患者CSF中の酸化ストレスマーカー、8-イソプロスタンF2αとPAFアセチルヒドロラーゼ活性の測定2008

    • Author(s)
      平島豊
    • Organizer
      第67回日本脳神経外科学会総会
    • Place of Presentation
      盛岡
    • Related Report
      2010 Final Research Report
  • [Presentation] 脳血管攣縮患者CSF中の酸化ストレスマーカー、8-イソブロスクンF2aとPAF-アセチルヒドロラーゼ活性の測定2008

    • Author(s)
      平島 豊
    • Organizer
      第67回日本脳神経外科学会総会
    • Place of Presentation
      盛岡
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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