Oxidative stress-induced apoptotic cell death pathway after spinal cord injury

• Project/Area Number: 20591704
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Akita University
• Principal Investigator: SUZUKI Akira Akita University, 大学院・医学系研究科, 助教 (10311573)
• Co-Investigator(Kenkyū-buntansha): SUGAWARA Taku 秋田大学, 医学部, 講師 (80241660)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脊髄損傷 / 運動ニューロン / アポトーシス / DNA損傷 / スーパーオキシド

Research Abstract

Ventral horn motor neurons (VMN) are selectively vulnerable to mild spinal cord injury (SCI) ; however, the mechanisms of cell death had not been understood. Mild compression SCI was induced in Sprague-Dawley rats, and superoxide production and apoptotic DNA injury were characterized. Increased superoxide production was observed exclusively in VMN after SCI. Subsequently, a majority of VMN (80%) selectively underwent delayed apoptotic cell death. These results suggest that the oxidative stress plays a pivotal role in apoptotic VMN death after SCI.

Research Products

• [Journal Article] Angiotensin receptor blocker directly binds to HSP and stimulates its production in the brain. Recent advances in mathematics and computers in business (2010)
  • Author(s): Sugawara T, Kokubun K., Ishida R., Fujiwara K.,Yamamoto S., Kinouchi H., Itoh H., Mizoi K.
  • Journal Title: economics, biology & chemistry Pages: 350-357
  • Peer Reviewed
• [Journal Article] Angiotensin receptor blocker directly binds to HSP and stimulates its production in the brain. (2010)
  • Author(s): Sugawara T, et al.
  • Journal Title: Recent advances in mathematics and computers in business, economics, biology & chemistry Volume: 1 Pages: 350-357
  • Peer Reviewed
• [Journal Article] Angiotensin receptor blocker candesartan binds to and upregulates molecular chaperone hsp90 in the hippocampal ca1 neurons -a possible mechanism of neuroprotection by angiotensin receptor blocker- (2009)
  • Author(s): Kokubun K, Sugawara T, Ishida R, Fujiwara K, Itoh H, Mizoi K
  • Journal Title: Akita J Med 36 Pages: 25-33
  • NAID: 110007118185
  • Peer Reviewed
• [Journal Article] Long term outcome and adjacent disc degeneration after anterior cervical disctomy and fusion with titanium cylindrical cages. (2009)
  • Author(s): Sugawara T, Itoh Y, Hirano Y, Higashiyama N, Mizoi K
  • Journal Title: Acta Neurochir 151 Pages: 303-309
  • Peer Reviewed
• [Journal Article] Limaprost alfadex improves myelopathy symptoms in patients with cervical spinal canal stenosis. (2009)
  • Author(s): Sugawara T, Hirano Y, Higashiyama N, Mizoi K
  • Journal Title: Spine 34(6) Pages: 551-555
  • NAID: 10027239605
  • Peer Reviewed
• [Journal Article] Limaprost alfadex improves myelopathy symptoms in patients with cervical spinal canal stenosis (2009)
  • Author(s): Sugawara T, Hirano Y, Higashiyama N, Mizoi K
  • Journal Title: Spine 34 Pages: 551-555
  • NAID: 10027239605
  • Peer Reviewed
• [Journal Article] Long term outcome and adjacent disc degeneration after anterior cervical discectomy and fusion with titanium cylindrical cages. (2009)
  • Author(s): Sugawara T, Itoh Y, Hirano Y, Higashiyama N, Mizoi K
  • Journal Title: Acta Neurochir 151 Pages: 303-309
  • Peer Reviewed
• [Journal Article] Negative Pressure Pulmonary Edema Following Foramen Magnum Decompression for Chiari Malformation Type 1 -case report- (2008)
  • Author(s): Hirano Y, Sugawara T, Sato Y, Sato K, Omae T, Sasajima T, Mizoi K
  • Journal Title: Neurol Med chir 48(3) Pages: 137-139
  • Peer Reviewed
• [Journal Article] 高齢者頸椎dumbell schwannoma の1手術例 (2008)
  • Author(s): 平野仁崇,菅原卓,高橋和孝,東山巨樹,山口卓,柴田憲一,西野克寛,笹嶋寿郎,溝井和夫
  • Journal Title: Geriatric Neurosurgery 20 Pages: 93-97
  • Peer Reviewed
• [Journal Article] Model mice for mild-form glycine encephalopathy : behavioral and biochemical characterizations and efficacy of antagonists for the glycine binding site of N-methyl D-aspartate receptor (2008)
  • Author(s): Kojima-ishii K, Kure S, Ichinohe A, Shinka T, Narisawa A, Komatsuzaki S, Kanno J, Kamada F, Aoki Y, Yokoyama H, Oda M, Sugawara T, Mizoi K, Nakahara D, Matsubara Y.
  • Journal Title: Pediatr Res 64 Pages: 228-233
  • Peer Reviewed
• [Presentation] 溝井和夫非機能性下垂体腺腫に対するfractionated sterotactic radiotherapy(FSRT) (2010)
  • Author(s): 鈴木明,羽入紀朋,笹嶋寿郎,古賀誠,高橋和孝,島田直也,柴田憲一,柳澤俊晴,橋本学
  • Organizer: の長期治療成績第19回日本定位放射線治療学会
  • Place of Presentation: 大津
  • Year and Date: 2010-07-30
• [Presentation] Invited lecture "Angiotensin receptor blocker directly binds to HSP and stimulates its production in the brain." (2010)
  • Author(s): 菅原卓
  • Organizer: WSEAS (World Scientific and Engineering Academy and Society) at Cambridge University
  • Place of Presentation: Cambridge (U.K.)
  • Year and Date: 2010-02-24
• [Presentation] Invited lecture "The recent progress in the research for cerebral ischemia" (2010)
  • Author(s): 菅原卓
  • Organizer: Lecture at Neuroscience Center, Genera University
  • Place of Presentation: Geneva (Switzerland)
  • Year and Date: 2010-02-22
• [Presentation] 頚椎前方アプローチの適応と基本手技 (2010)
  • Author(s): 菅原卓
  • Organizer: 第30回日本脳神経外科コングレス総会
  • Place of Presentation: 横浜
• [Presentation] 溝井和夫脊髄髄内病変の手術経験 (2010)
  • Author(s): 菅原卓,東山巨樹,柴田憲一,高橋和孝,笹嶋寿郎
  • Organizer: 第25回日本脊髄外科学会
  • Place of Presentation: 名古屋
• [Presentation] 非機能性下垂体腺腫に対するfanctionated stereotactic radiotherapy (FSRT)の長期治療成績 (2010)
  • Author(s): 鈴木明,羽入紀朋,笹嶋寿郎,高橋和孝,島田直也,柴田憲一,溝井和夫,古賀眞,橋本学
  • Organizer: 第69回日本脳神経外科学会総会
  • Place of Presentation: 福岡
• [Presentation] スクリューガイドテンプレートシステムを用いた頚椎後方固定術 (2010)
  • Author(s): 菅原卓,東山巨樹,大野秀則,杉山久幸,溝井和夫
  • Organizer: 第69回日本脳神経外科学会総会
  • Place of Presentation: 福岡
• [Presentation] 頭蓋底部髄膜腫に対するstereotactic radiotherapy(SRT)の治療成績 (2009)
  • Author(s): 鈴木明,羽入紀朋,佐久間郁郎,高橋和孝,柳澤俊晴,溝井和夫
  • Organizer: 第18回日本定位放射線治療学会
  • Place of Presentation: 仙台
  • Year and Date: 2009-07-17
• [Presentation] 特別講演「脳卒中患者におけるARB の投与意義」 (2009)
  • Author(s): 菅原卓
  • Organizer: 第50回日本神経学会総会イブニングセミナー
  • Place of Presentation: 仙台
  • Year and Date: 2009-05-21
• [Presentation] 単一神経根に多発した硬膜内神経鞘腫の2手術例 (2009)
  • Author(s): 菅原卓,東山巨樹,溝井和夫
  • Organizer: 第24回日本脊髄外科学会
  • Place of Presentation: 宮崎
• [Presentation] キアリI型奇形に対するsandwith duraplasty (2009)
  • Author(s): 菅原卓,東山巨樹,平野仁崇,柴田憲一,伊藤康信,溝井和夫
  • Organizer: 第68回日本脳神経外科学会総会
  • Place of Presentation: 東京
• [Presentation] 頭蓋底部髄膜腫に対するstereotactic radiotherapy(SRT)の治療成績 (2009)
  • Author(s): 鈴木明,羽入紀朋,佐久間郁郎,高橋和孝,柳澤俊晴,溝井和夫
  • Organizer: 第68回日本脳神経外科学会総会
  • Place of Presentation: 東京
• [Presentation] キアリI型奇形に対するsandwith duraplasty (2009)
  • Author(s): 菅原卓,東山巨樹,平野仁崇,柴田憲一,伊藤康信,溝井和夫
  • Organizer: 第44回日本脊髄障害医学会
  • Place of Presentation: 東京
• [Presentation] 聴神経腫瘍に対するLinac によるstereotactic radiotherapy(SRT)の治療成績 (2008)
  • Author(s): 鈴木明,柳澤俊晴,高橋和孝,太田徹,内藤雄一郎,笹嶋寿郎,溝井和夫,泉純一,戸村則昭,渡会二郎
  • Organizer: 第17回日本定位放射線治療学会
  • Place of Presentation: 奈良
  • Year and Date: 2008-05-30
• [Presentation] 頚椎症性脊髄症に対するリマプロストの効果 (2008)
  • Author(s): 菅原卓,平野仁崇,東山巨樹,国分康平,大前智也,島田直也,溝井和夫
  • Organizer: 第23回日本脊髄外科学会
  • Place of Presentation: 松島
• [Presentation] 頚椎前方除圧固定術におけるチタンケージ充填材料としてのβ-TCPとアパセラムの比較 (2008)
  • Author(s): 菅原卓,東山巨樹,平野仁崇,溝井和夫
  • Organizer: 第67回(社)日本脳神経外科学会総会
  • Place of Presentation: 盛岡
• [Presentation] 聴神経腫瘍に対するLinac によるfractionated stereotactic radiotherapy(FSRT)の治療成績 (2008)
  • Author(s): 鈴木明,羽入紀朋,佐久間郁郎,高橋和孝,柳澤俊晴,溝井和夫
  • Organizer: 第67回(社)日本脳神経外科学会総会
  • Place of Presentation: 盛岡
• [Presentation] 頚椎症性脊髄症に対するリマプロストの効果 (2008)
  • Author(s): 菅原卓,平野仁崇,東山巨樹,溝井和夫
  • Organizer: 第43回日本脊髄障害医学会
  • Place of Presentation: 札幌
• [Remarks] ホームページ等