Genetic analysis of glioblastoma cancer stem cell with special reference to chronological and spatial change

• Project/Area Number: 20591708
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Kanazawa University
• Principal Investigator: HAYASHI Yutaka Kanazawa University, 医学系, 准教授 (90262568)
• Research Collaborator: NAKADA Mitsutoshi 金沢大学, 医学系, 助教 (20334774) ; KITA Daisuke 金沢大学, 医学系, 助教 (10377385)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脳腫瘍 / 神経膠芽腫 / 腫瘍幹細胞 / 神経膠芽腫瘍 / 遺伝子異常

Research Abstract

To evaluate the specific factors in tumorigenesis of the glioblastoma, especially in chronological and spatial character, several genes expressions and mutations were analyzed. Among the integrins that could be related with invasion, the alpha 3 subunit had a significantly higher expression level in tumor stem cells. The alpha 3 subunit of integrin could play an important role in the tumor invasion. Furthermore, during a course of tumor progression, FAS and TP53 mutation were detected. These genetic alterations could be related with tumor progression with a malignant transformation.

Research Products

• [Journal Article] The correlation between promoter methylation status and the expression level of O6-methylguanine-DNA methyltransferasein recurrent glioma. (2011)
  • Author(s): Suzuki, T., Nakada, M., Yoshida, Y., Nambu, E., Furuyama, N., Kita, D., Hayashi, Y., Hayashi, Y., Hamada, JI.
  • Journal Title: Jpn J Clin Oncol 41 Pages: 190-196
  • Peer Reviewed
• [Journal Article] Secondary anaplastic astrocytoma developing in a young adult with autoimmune lymp hoproliferative syndrome (2011)
  • Author(s): Kita D., et al
  • Journal Title: Neuropathol Appl Neurobiol Volume: in press
  • Peer Reviewed
• [Journal Article] Epithelioid glioblastoma changed to typical glioblastoma : the methylation status of MGMT promoter and 5-ALA fluorescence (2011)
  • Author(s): Tanaka S., et al
  • Journal Title: Brain Tumor Pathol Volume: in press
  • Peer Reviewed
• [Journal Article] The expression level of sphingosine-1-phosphate receptor type 1 is related to MIB-1 labeling index and predicts survival of glioblastoma patients. (2010)
  • Author(s): Yoshida, Y., Nakada, M., Harada, T., Tanaka, S., Furuta, T., Hayashi, Y., Kita, D., Uchiyama, N., Hayashi, Y., Hamada, JI.
  • Journal Title: J Neurooncol 98 Pages: 41-47
  • Peer Reviewed
• [Journal Article] Implication of 5-aminolevulinic acid fluorescence of the ventricular wall for postoperative communicating hydrocephalus associated with cerebrospinal fluid dissemination in patients with glioblastoma multiforme : a report of 7 cases. (2010)
  • Author(s): Hayashi, Y., Nakada, M., Tanaka, S., Uchiyama, N., Hayashi, Y., Kita, D., Hamada, JI.
  • Journal Title: J Neurosurg 112 Pages: 1015-1019
  • Peer Reviewed
• [Journal Article] The correlation between promoter methylation status and the expression level of O6-methylguanine-DNA methyltrans ferase in recurrent glioma (2010)
  • Author(s): Suzuki T., et al
  • Journal Title: Jpn J Clin Oncol I Volume: 41 Pages: 190-196
  • Peer Reviewed
• [Journal Article] Ependymoma and choroid plexus papilloma as synchronous multiple neuroepithelial tumor in the same patient : a case report and review of literature (2010)
  • Author(s): Hayashi Y., et al
  • Journal Title: Neurosurgery (in press)
  • Peer Reviewed
• [Journal Article] The expression level of sphingosine-1-phosphate receptor type 1 is related to MIB-1 labeling index and predicts survival of glioblastoma patients (2010)
  • Author(s): Yoshida Y., et al
  • Journal Title: J Neurooncol (in press)
  • Peer Reviewed
• [Journal Article] Implication of 5-aminolevulinic acid fluorescence of the ventricular wall for postoperative communicating hydrocephalus associated with cerebrospinal fluid dissemination in patients with glioblastoma multiforme : a report of 7 cases (2010)
  • Author(s): Hayashi Y., et al
  • Journal Title: J Neurosurg (in press)
  • Peer Reviewed
• [Journal Article] BCL10 single nucleotide polymorphism and its association with CSF dissemination of primary intracranial germ cell tumors (2009)
  • Author(s): Hayashi, Y., Watanabe, T., Nakada, M., Kita, D., Hayashi, Y., Uchiyama, N., Hamada, JI.
  • Journal Title: Pediatr Neurosurg 45 Pages: 291-295
  • Peer Reviewed
• [Journal Article] Potential therapeutic effect of glycogen synthase kinase 3beta inhibition against human glioblastoma. (2009)
  • Author(s): Miyashita, K., Kawakami, K., Nakada, M., Mai, W., Shakoori, A., Fujisawa, H., Hayashi, Y., Hamada, JI., Minamoto, T.
  • Journal Title: Clin Cancer Res 15 Pages: 887-897
  • Peer Reviewed
• [Journal Article] A reevaluation of the primary diagnosis of hemangiopericytoma and the clinical importance of differential diagnosis from solitary fibrous tumor of the central nervous system. (2009)
  • Author(s): Hayashi, Y., Uchiyama, N., Hayashi, Y., Nakada, M., Iwato, M., Kita, D., Higashi, R., Hirota, Y., Kai, Y., Kuratsu, J., Hamada, JI.
  • Journal Title: Clin Neurol Neurosurg 111 Pages: 34-38
  • Peer Reviewed
• [Journal Article] 遺伝子解析が膠芽腫亜分類に与えたインパクト (2008)
  • Author(s): 林裕
  • Journal Title: Journal of the Juzen Medical Society(金沢大学十全医学会雑誌) 117 Pages: 89-93
  • NAID: 10026960627
• [Journal Article] Ependymoma and choroid plexus papilloma as synchronous multiple neuroepithelial tumors in the same patient : a case report and review of literature.
  • Author(s): Hayashi, Y., Mohri, M., Nakada, M., Hamada, JI.
  • Journal Title: Neurosurgery in press
  • Peer Reviewed
• [Journal Article] Secondary anaplastic astrocytoma developing in a young adult with autoimmune lymphoproliferative syndrome (ALPS).
  • Author(s): Kita, D., Hayashi, Y., Watanabe, T., Korshunov, A., vonDeimling, A., Nakada, M., Kasahara, Y., Zen, Y., Hamada, JI., Hayashi, Y.
  • Journal Title: Neuropathol Appl Neurobiol in press
  • Peer Reviewed
• [Presentation] 膠芽腫におけるGSK3 β研究の進展とその阻害薬剤を使用した再発膠芽腫に対する第I/II相臨床試験 (2010)
  • Author(s): 中田光俊、林裕、宮下勝吉、渡邉卓也、Pyko IIya、喜多大輔、近野祐里、林康彦、内山尚之、川上和之、源利成、濱田潤一郎
  • Organizer: 第69回日本脳神経外科学会学術総会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2010-10-29
• [Presentation] 頭蓋咽頭腫に対するtranscrusal approachにおいて三半規管削除が術野に及ぼす影響 (2010)
  • Author(s): 林裕、木下雅史、中田光俊、毛利正直、林康彦、内山尚之、濱田潤一郎
  • Organizer: 第69回日本脳神経外科学会学術総会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2010-10-28
• [Presentation] 神経膠腫におけるIDH1/2遺伝子変異の意義 (2010)
  • Author(s): 渡邉卓也、信澤純人、喜多大輔、中田光俊、林裕、濱田潤一郎、大垣比呂子
  • Organizer: 第69回日本脳神経外科学会学術総会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2010-10-27
• [Presentation] 巨大insular glioma摘出の問題点 (2010)
  • Author(s): 林裕、中田光俊、喜多大輔、佐野宏樹、田中慎吾、長谷川貴之、林康彦、内山尚之、濱田潤一郎
  • Organizer: 第15回日本脳腫瘍の外科学会
  • Place of Presentation: 大阪市中央公会堂(大阪府)
  • Year and Date: 2010-10-02
• [Presentation] 巨大insular glioma摘出の問題点 (2010)
  • Author(s): 林裕, 他
  • Organizer: 第15回日本脳腫瘍の外科学会
  • Place of Presentation: 大阪中央公会堂(大阪)
• [Presentation] Non-enhancing intrinsic tumor (NEIT)において摘出率が予後に及ぼす影響と術中モニタリングの重要性 (2009)
  • Author(s): 林裕、中田光俊、田中慎吾、上出智也、林康彦、内山尚之、濱田潤一郎
  • Organizer: 第68回日本脳神経外科学会学術総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2009-10-15
• [Presentation] Frequent single nucleotide polymorphism (SNP) of BCL10 and association with poor prognosis in primary intracranial germ cell tumors (2009)
  • Author(s): 林裕, 渡邉卓也, 中田光俊, 喜多大輔, 林康彦, 内山尚之, 濱田潤一郎
  • Organizer: 3^<rd> Quadrennial Meeting of the World Federation of Neuro-Oncology
  • Place of Presentation: PACIFICO YOKOHAMA (JAPAN)
  • Year and Date: 2009-05-12
• [Presentation] Non-enhancing intrinsic tumor (NEIT) において摘出率が予後に及ぼす影響と術中モニタリングの重要性 (2009)
  • Author(s): 林裕, 他
  • Organizer: 社団法人日本脳神経外科学会第68回学術総会
  • Place of Presentation: (東京) 京王プラザホテル
• [Presentation] 坐位手術の有用性 (2008)
  • Author(s): 林裕、中田光俊、木下雅史、内山尚之、新多寿、長谷川光広、山下純宏、濱田潤一郎
  • Organizer: 第67回日本脳神経外科学会学術総会
  • Place of Presentation: 岩手県民会館(岩手県)
  • Year and Date: 2008-10-02
• [Book] ビジュアル脳神経外科1、前頭葉・頭頂葉 (2010)
  • Author(s): 林裕、濱田潤一郎
  • Publisher: MEDICAL VIEW
• [Book] RNA technologies and their applications (2010)
  • Author(s): Nakada M, Kita D, Hayashi Y, Kawakami K, Hamada JI, Minamoto T
  • Publisher: Springer Verlag
• [Patent(Industrial Property Rights)] 脳腫瘍治療用キット及び脳腫瘍治療方法 (2010)
  • Inventor(s): 中田光俊、源利成、林裕、濱田潤一郎
  • Industrial Property Rights Holder: 金沢大学
  • Industrial Property Number: 2010-185691
  • Filing Date: 2010-08-22