Identification of epigenetic biomarkers in benign brain tumors in the post-genome era

• Project/Area Number: 20591710
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Nagoya University
• Principal Investigator: SAITO Kiyoshi Nagoya University, 医学部, 教授 (00240804)
• Co-Investigator(Kenkyū-buntansha): NATSUME Atsushi 名古屋大学, 大学院・医学系研究科, 准教授 (30362255)
• Research Collaborator: ITO Eiji 福島医科大学, 医学部, 助教 (30402605) ; KISHIDA Yugo 名古屋大学, 大学院・医学系研究科, 大学院
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 良性脳腫瘍 / 髄膜腫 / エピジェネティクス / マイクロアレイ / DNAメチル化 / バイオマーカー / エピジェティクス / 動物モデル

Research Abstract

We analyzed exhaustive epigenetic changes of the meningioma, the most common brain tumor. Using microarrays that can analyze 6157 genomes, 15273 CpG islands, 20 WHO grade 1 and 10 grade 2 meningiomas were analyzed. The data showed 2 clusters, hypo-methylated and hyper-methylated groups, independent of WHO grading. Most invasive meningiomas and recurrent meningiomas were included in the hyper-methylated cluster. Our results suggest that epigenetic changes of DNA methylation reflect active nature, such as invasion or recurrence, of the meningiomas.

Research Products

• [Journal Article] 頭蓋底髄膜腫に対する外科治療の役割:海綿静脈洞～petroclival meningiomaを中心に (2011)
  • Author(s): 齋藤清, 伊藤英治, 佐藤拓, 市川優寛, 渡邉督, 松本由香, 安藤等, 織田恵子, 佐久間潤
  • Journal Title: 脳神経外科ジャーナル Volume: 20 Pages: 170-176
  • Peer Reviewed
• [Journal Article] Epigenetic aberrations and therapeutic implications in gliomas. (2010)
  • Author(s): Natsume A, Kondo Y, Ito M, Motomura K, Wakabayashi T, Yoshida J.
  • Journal Title: Cancer Sci 101 Pages: 1331-1336
  • NAID: 10026592977
  • Peer Reviewed
• [Journal Article] 髄膜腫治療の問題点 (2010)
  • Author(s): 齋藤清, 伊藤英治, 市川優寛, 佐藤拓, 渡邉督, 佐久間潤
  • Journal Title: 「新時代の脳腫瘍学」日本臨床 68増刊号 10 Pages: 324-328
• [Journal Article] Efficient delivery of liposome-mediated MGMT-siRNA reinforces the cytotoxity of temozolomide in GBM-initiating cells. (2010)
  • Author(s): Kato T, Natsume A, Toda H, Iwamizu H, Sugita T, Hachisu R, Watanabe R, Yuki K, Motomura K, Bankiewicz K, Wakabayashi T
  • Journal Title: Gene Therapy 17 Pages: 1363-1371
  • Peer Reviewed
• [Journal Article] Efficient delivery of liposome-mediated MGMT-siRNA reinforces the cytotoxity of temozolomide in GBM-initiating cells (2010)
  • Author(s): Kato T, Natsume A, Toda H, Iwamizu H, Sugita T, Hachisu R, Watanabe R, Yuki K, Motomura K, Bankiewicz K, Wakabayashi T
  • Journal Title: Gene Therapy Volume: 17 Pages: 1363-1371
  • Peer Reviewed
• [Journal Article] A free-radical scavenger protects the neural progenitor cells in the dentate subgranular zone of the hippocampus from cell death after X-irradiation (2010)
  • Author(s): Motomura K, Ogura M, Natsume A, Yokoyama H, Wakabayashi T
  • Journal Title: Neurosci Lett Volume: 485 Pages: 65-70
  • Peer Reviewed
• [Journal Article] Retrovirally engineered T-cell-based immunotherapy targeting type-III variant epidermal growth factor receptor, a glioma-associated antigen (2010)
  • Author(s): Ohno M, Natsume A, Iwami K, Iwamizu H, Noritake K, Ito D, Toi Y, Ito M, Kazuya M, Yoshida J, Yoshikawa K Wakabayashi T
  • Journal Title: Cancer Sci Volume: 101 Pages: 2518-2524
  • NAID: 10027834208
  • Peer Reviewed
• [Journal Article] Epigenetic aberrations and therapeutic implications in gliomas (2010)
  • Author(s): Natsume A, Kondo Y, Ito M, Motomura K, Wakabayashi T, Yoshida J
  • Journal Title: Cancer Sci Volume: 101 Pages: 1331-1336
  • NAID: 10026592977
  • Peer Reviewed
• [Journal Article] Human neural stem cells transduced with IFN-beta and cytosine deaminase genes intensify bystander effect in experimental glioma (2010)
  • Author(s): Ito S, Natsume A, Shimato S, Ohno M, Kato T, Chansakul P, Wakabayashi T, Kim SU
  • Journal Title: Cancer Gene Therapy Volume: 17 Pages: 299-306
  • Peer Reviewed
• [Journal Article] Intradural cranial chordoms (2010)
  • Author(s): Ito E, Saito K, Nagatani T, Ishiyama J, Terada K, Yoshida M, Wakabayashi T
  • Journal Title: World Neurosurg Volume: 73 Pages: 194-197
  • Peer Reviewed
• [Journal Article] Magnetically guided 3-dimensional virtual neuronavigation for neuroendoscopic surgery : technique and clinical experience (2010)
  • Author(s): Ito E, Fujii M, Hayashi Y, Zhengang J, Nagatani T, Satio K, Kishida Y, Mori K, Wakabayashi T
  • Journal Title: Neurosurgery Volume: 66 Pages: 342-353
  • Peer Reviewed
• [Journal Article] 髄膜腫治療の問題点.「新時代の脳腫瘍学」 (2010)
  • Author(s): 齋藤清, 伊藤英治, 市川優寛, 佐藤拓, 渡邉督, 佐久間潤
  • Journal Title: 日本臨床 Volume: 68増刊号10 Pages: 324-328
• [Journal Article] Iatrogenic drop metastasis of recurrent meningioma in a vascularized free omental flap (2010)
  • Author(s): Ichikawa M, Saito K, Ito E, Kawai K, Fujimoto Y, Kamei Y
  • Journal Title: Neurol Med Chir (Tokyo) 50 Pages: 70-73
  • Peer Reviewed
• [Journal Article] Long-term control of clival chordoma with initial aggressive surgical resection and gamma knife radiosurgery for recurrence (2010)
  • Author(s): Ito E, Saito K Okada T, Nagatani T, Nagasaka T
  • Journal Title: Acta Neurochir 152 Pages: 57-67
  • Peer Reviewed
• [Journal Article] p16 promoter methylation in the serum as a basis for the molecular diagnosis of gliomas. (2009)
  • Author(s): Wakabayashi T, Natsume A, Hatano H, Fujii M, Shimato S, Ito M, Ohno M, Ito S, Ogura M, Yoshida J.
  • Journal Title: Neurosurgery 64 Pages: 455-461
  • Peer Reviewed
• [Journal Article] Synergistic induction of NY-ESO-1 antigen expression by a novel histone deacetylase inhibitor, valproic acid, with 5-aza-2'-deoxycytidine in glioma cells. (2009)
  • Author(s): Oi S, Natsume A, Ito M, Kondo Y, Shimato S, Maeda Y, Saito K, Wakabayashi T
  • Journal Title: J Neurooncol 92 Pages: 15-22
  • Peer Reviewed
• [Journal Article] Factors predicting growth of vestibular schwannoma in neurofibromatosis type 2 (2009)
  • Author(s): Ito E, Saito K Yatsuya H, Nagatani T, Otsuka G
  • Journal Title: Neurosurg Rev 32 Pages: 425-433
  • Peer Reviewed
• [Journal Article] The modulation of MicroRNAs by type I IFN through the activation of signal transducers and activators of transcription 3 in human glioma (2009)
  • Author(s): Ohno M, Natsume A Kondo Y, Iwamizu H, Motomura K, Toda H, Ito M, Kato T, Wakabayashi T
  • Journal Title: Mol Cancer Res 7 Pages: 2002-2030
  • Peer Reviewed
• [Journal Article] Induction of oligodendrogenesis in glioblastoma-initiating cells by IFN-mediated activation of STAT3 signaling (2009)
  • Author(s): Yuki K, Natsume A, Yokoyama H, Kondo Y, Ohno M, Kato T, Chansakul P, Ito M, Kim SU, Wakabayashi T
  • Journal Title: Cancer Lett 284 Pages: 71-79
  • Peer Reviewed
• [Journal Article] Interferon-beta, MCNU, and conventional radiotherapy for pediatric patients with brainstem glioma (2009)
  • Author(s): Ohno M, Natsume A Fujii M, Ito M, Wakabayashi T
  • Journal Title: Pediatr Blood Cancer 53 Pages: 37-41
  • Peer Reviewed
• [Journal Article] p16 promoter methylation in the serum as a basis for the molecular diagnosis of gliomas (2009)
  • Author(s): Wakabayashi T, Natsume A Hatano H, Fujii M, Shimato S, Ito M, Ohno M, Ito S, Ogura M, Yoshida J
  • Journal Title: Neurosurgery 64 Pages: 455-461
  • Peer Reviewed
• [Journal Article] Type I interferon inhibits astrocytic gliosis and promotes functional recovery after spinal cord injury by deactivation of the MEK/ERK pathway (2009)
  • Author(s): Ito M, Natsume A, Takeuchi H, Shimato S, Ohno M, Wakabayashi T, Yoshida J
  • Journal Title: J Neurotrauma 26 Pages: 41-53
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 齋藤清, 藤本保志
  • Journal Title: 頭蓋底腫瘍.「脳神経外科周術期管理のすべて 改訂第3版:松谷雅生,田村晃(編)」(メディカルビュー,東京) Pages: 167-175
• [Journal Article] (2009)
  • Author(s): 齋藤清, 伊藤英治
  • Journal Title: Rathke嚢胞.「下垂体腫瘍のすべて:寺本明,長村義之(編)」(医学書院,東京) Pages: 282-287
• [Journal Article] (2009)
  • Author(s): 齋藤清
  • Journal Title: 前頭蓋底の解剖と手術.「脳神経外科エキスパート 頭蓋底:佐伯直勝(編)」(中外医学社,東京) Pages: 38-47
• [Journal Article] Synergistic induction of NY-ESO-1antigen expression by a novel histone deacetylase inhibitor, valproic acid, with 5-aza-2'-deoxyoytidine in glioma cells. (2009)
  • Author(s): Oi S, Natsume A. Ito M, Kondo Y, Shimato S, Maeda Y, Saito K, Wakabayashi T.
  • Journal Title: J Neurooncol. 92 Pages: 15-22
  • Peer Reviewed
• [Journal Article] Preliminary measurement of intraoperative sympathetic nerve acticity using microneurography and laser Doppler flowmetry during surgical resection of suprasellar tumors. (2009)
  • Author(s): Kurimoto F, Saito K, Watanabe T, Nagatani T, Nishiwaki K, Wakabayashi T. Iwase S.
  • Journal Title: Neurol Med Chir (Tokyo). 49 Pages: 13-21
  • Peer Reviewed
• [Journal Article] Measurement of optic nerve blood flow during dissection of parasellar tumors. (2009)
  • Author(s): Aimi Y, Saito K, Nagatani T, Ito E, Watanabe T, Wakabayashi T.
  • Journal Title: Neurosurg Review. 32 Pages: 199-205
  • NAID: 120002386546
  • Peer Reviewed
• [Journal Article] Lymphangioma of the skull base bones leading to cerebrospinal fluid rhinorrhea. (2008)
  • Author(s): Ito E, Saito K, Nagatani T, Teranishi M, Kamei Y, Yagi S, Kawabe T, Niimi N, Yoshida J.
  • Journal Title: J Neurosurg Pediatr. 2 Pages: 273-276
  • Peer Reviewed
• [Journal Article] The DNA demethylating agent 5-aza-2'-deoxycytidine activates NY-ESO-1 antigenicity in orthotopic human glioma. (2008)
  • Author(s): Natsume A, Wakabayashi T, Tsujimura K, Shimato S, Ito M, Kuzushima K, Kondo Y, Sekido Y, Kawatsura H, Narita Y, Yoshida J
  • Journal Title: Int J Cancer. 122 Pages: 2542-2553
  • Peer Reviewed
• [Presentation] Aggressive resection of invasive skull base meningiomas. (2011)
  • Author(s): 齋藤清, et al.
  • Organizer: North American Skull Base Society 21st Annual Meeting
  • Place of Presentation: Scottsdale, USA
• [Presentation] Aggressive resection of invasive skull base meningiomas (2011)
  • Author(s): Saito K, Sato T, Ito E, Watanabe T, Ichikawa M, Oda K, Ando H, Matsumoto Y, Sakuma J
  • Organizer: North American Skull Base Society 21st Annual Meeting
  • Place of Presentation: Scottsdale, USA
• [Presentation] エピゲノムの見地からみた髄膜腫の再分類 (2010)
  • Author(s): 岸田悠吾, 夏目敦至, 近藤豊, 齋藤清, 若林俊彦
  • Organizer: 第28回日本脳腫瘍学会
  • Place of Presentation: 軽井沢
• [Presentation] 髄膜腫のエピゲノム解析 (2010)
  • Author(s): 岸田悠吾, 夏目敦至, 近藤豊, 齋藤清, 若林俊彦
  • Organizer: 第69回日本脳神経外科学会総会
  • Place of Presentation: 福岡
• [Presentation] 頭蓋底髄膜腫に対する外科治療の役割:海綿静脈洞～petroclival meningiomaを中心に (2010)
  • Author(s): 齋藤清, 伊藤英治, 佐藤拓, 市川優寛, 渡邉督, 佐久間潤
  • Organizer: 第30回 日本脳神経外科コングレス
  • Place of Presentation: 横浜
• [Presentation] 篩骨洞悪性腫瘍に対する-塊切除再建術と長期成績 (2010)
  • Author(s): 齋藤清, 伊藤英治, 佐藤拓, 市川優寛, 渡邊督, 佐久間潤, 長谷川泰久, 高橋正克, 藤本保志, 亀井譲
  • Organizer: 第22回 日本頭蓋底外科学会
  • Place of Presentation: 久留米
• [Presentation] 髄膜腫のエピゲノム解析 (2010)
  • Author(s): 岸田悠吾, 夏目敦至, 近藤豊, 齋藤清, 若林俊彦
  • Organizer: 第69回日本分子脳神経外科学会
  • Place of Presentation: 仙台
• [Presentation] Bifrontal basal interhemispheric approachによる頭蓋咽頭腫の摘出術 (2010)
  • Author(s): 齋藤清, 佐藤拓, 渡邉督, 市川優寛, 伊藤英治, 織田恵子, 松本由香, 安藤等, 佐久間潤
  • Organizer: 第15回 日本脳腫瘍の外科学会
  • Place of Presentation: 大阪
• [Presentation] Strategies for malignant skull base tumors (2010)
  • Author(s): Saito K, Ichikawa M, Ito E, Sato T, Watanabe T, Oda K, Ando H, Matsumoto Y, Sakuma J
  • Organizer: 12^<th> Annual Conference of the Skull Base Surgery Society of India
  • Place of Presentation: Trivandrum, India
• [Presentation] Strategies for malignant skull base tumors (2010)
  • Author(s): Saito K, Ito E, S ato T, Watanabe T, Ichikawa M, Oda K, Ando H, Matsumoto Y, Sakuma J
  • Organizer: 4^<th> Indian Japanese Friendship Neurosurgical Meeting
  • Place of Presentation: Cochin, India
• [Presentation] 髄膜腫のエピゲノム解析 (2010)
  • Author(s): 岸田悠吾, 夏目敦至, 近藤豊, 齋藤清, 若林俊彦
  • Organizer: 第69回 日本脳神経外科学会総会
  • Place of Presentation: 福岡
• [Presentation] 全摘出を基本とした頭蓋咽頭腫の治療戦略と成績 (2010)
  • Author(s): 齋藤清, 渡邉督, 佐藤拓, 市川優寛, 伊藤英治, 安藤等, 松本由香, 佐久間潤, 永谷哲也, 若林俊彦
  • Organizer: 第69回 日本脳神経外科学会総会
  • Place of Presentation: 福岡
• [Presentation] Aggressive resection of invasive skull base meningiomas (2010)
  • Author(s): Saito K, Ito E, Watanabe T, Sato T, Ichikawa M, Oda K, Ando H, Matsumoto Y, Sakuma J
  • Organizer: 10^<th> Asian-Oceanian International Congress on Skull Base Surgery
  • Place of Presentation: Bali, Indonesia
• [Presentation] Aggressive resection and long-term outcome of skull base chordomas (2010)
  • Author(s): Saito K, Ito E, Watanabe T, Sato T, Ichikawa M, Oda K, Ando H, Matsumoto Y, Sakuma J.
  • Organizer: 10^<th> Asian-Oceanian International Congress on Skull Base Surgery
  • Place of Presentation: Bali, Indonesia
• [Presentation] エピゲノムの見地からみた髄膜腫の再分類 (2010)
  • Author(s): 岸田悠吾, 夏目敦至, 近藤豊, 齋藤清, 若林俊彦
  • Organizer: 第28回 日本脳腫瘍学会
  • Place of Presentation: 軽井沢
• [Presentation] 再発間葉性軟骨肉腫に対する開頭・経鼻内視鏡併用頭蓋底手術 (2010)
  • Author(s): 齋藤清, 渡邉督, 粕谷泰道, 伊藤英治, 佐藤拓, 市川優寛, 佐久間潤
  • Organizer: 第26回 白馬脳神経外科セミナー
  • Place of Presentation: 白馬
• [Presentation] 頭蓋底髄膜腫の手術 (2009)
  • Author(s): 齋藤清
  • Organizer: 第45回千葉神経外科研究会
  • Place of Presentation: 千葉
  • Year and Date: 2009-01-30
• [Presentation] Skull base surgeries and long-term outcome for malignant skull base tumors. (2009)
  • Author(s): 齋藤清, et al.
  • Organizer: XIV World Congress of Neurological Surgery of the World Federation of Neurosurgical Societies
  • Place of Presentation: Boston, USA
• [Presentation] Skull base approaches for and long term control of clival chordomas (2009)
  • Author(s): Saito K, Ito E, Okada T, Aimi Y, Nagatani T
  • Organizer: 9^<th> Congress of the European Skull Base Society
  • Place of Presentation: Rotterdam, Netherlands
• [Presentation] トルコ鞍近傍部腫瘍の手術:開頭による摘出術 (2009)
  • Author(s): 齋藤清
  • Organizer: 第29回 日本脳神経外科コングレス
  • Place of Presentation: 大阪
• [Presentation] 前頭蓋底・眼窩の脳外科手術 (2009)
  • Author(s): 齋藤清
  • Organizer: 第21回 日本頭蓋底外科学会
  • Place of Presentation: 福岡
• [Presentation] Skull base surgeries and long-term outcome for malignant skull base tumors (2009)
  • Author(s): Saito K, Ito E, Okada T, Tachibana E, Nagatani T, Wakabayashi T
  • Organizer: XIV World Congress of Neurological Surgery of the World Federation of Neurosurgical Societies
  • Place of Presentation: Boston, USA
• [Presentation] 静脈洞に進展した髄膜腫の摘出術 (2009)
  • Author(s): 齋藤清, 伊藤英治, 佐藤拓, 粕谷泰道, 安藤等, 松本由香, 渡邉督, 佐久間潤、永谷哲也、若林俊彦
  • Organizer: 第14回 日本脳腫瘍の外科学会
  • Place of Presentation: 東京
• [Presentation] Clinoidal meningiomaの手術 (2009)
  • Author(s): 齋藤清, 伊藤英治, 佐藤拓, 粕谷泰道, 安藤等, 松本由香, 渡邉督, 佐久間潤, 永谷哲也, 若林俊彦
  • Organizer: 第68回 社団法人日本脳神経外科学会総会
  • Place of Presentation: 東京
• [Presentation] Aggressive removal of malignant skull base tumors : cancers, chordomas, and invasive or malignant skull base meningiomas (2009)
  • Author(s): Saito K
  • Organizer: 49^<th> Annual Meeting of the Korean Neurosurgical Society
  • Place of Presentation: Seoul, Korea
• [Presentation] 経頭蓋硬性鏡単独手術 (2009)
  • Author(s): 齋藤清, 渡邉督, 安藤等, 粕谷泰道, 伊藤英治, 佐藤拓, 市川優寛, 佐久間潤, 永谷哲也
  • Organizer: 第16回 日本神経内視鏡学会
  • Place of Presentation: 富山
• [Presentation] Surgical treatment of invasive and atypical skull base meningiomas (2009)
  • Author(s): Saito K, Sato T, Ito E, Kasuya H, Ichikawa M, Watanabe T, Sakuma J
  • Organizer: 58^<th> Annual Conference of Neurological Society of India
  • Place of Presentation: Lucknow, India
• [Presentation] Genetic characteristics of malignant meningiomas. (2008)
  • Author(s): 夏目敦至, 齋藤清, et al.
  • Organizer: 6th International Congress on Meningiomas and Cerebral Venous System
  • Place of Presentation: Boston, USA
• [Presentation] Outcome after aggressive removal of invasive skull base meningiomas. (2008)
  • Author(s): Ichikawa M, Saito K, Ito E, Okada T, Watanebe T, Nagatani T.
  • Organizer: 9^<th> Asian-Oceanian International Congress on Skull Base Surgery
  • Place of Presentation: Busan, Korea
• [Presentation] standard procedures for malignant skull base tumors ; from experience of 150 patients. (2008)
  • Author(s): Saito K, Tachibana E, Okada T, Ito E, Fujimoto Y, Kamei Y.
  • Organizer: 5^<th> international congress of the world federation of skull base societies
  • Place of Presentation: Vancouver, Canada
• [Presentation] 浸潤性頭蓋底髄膜腫の長期成績と治療戦略. (2008)
  • Author(s): 齋藤清, 永谷哲也, 伊藤英治, 渡邊督, 前澤聡, 中原紀元, 岡田健, 八木俊路朗, 亀井譲.
  • Organizer: 第20回 日本頭蓋底外科学学会
  • Place of Presentation: 東京
• [Book] イラストレイテッド脳腫瘍外科学(小脳橋角部髄膜腫) (2011)
  • Author(s): 齋藤清
  • Publisher: 医学書院
• [Book] 小脳橋角部髄膜腫.頭蓋底の修復と閉鎖.イラストレイテッド脳腫瘍外科学:河本圭司,本郷一博,栗栖薫(編) (2011)
  • Author(s): 齋藤清
  • Publisher: 医学書院,東京
• [Book] 脳腫瘍の外科Science, Art and Technology(静脈洞に進展した髄膜腫の摘出術) (2010)
  • Author(s): 齋藤清, ら
  • Publisher: 編集室なるにあ
• [Book] 錐体斜台部・錐体部髄膜腫の手術-retrosigmoid approach. NS NOW 10後頭蓋窩手術 これだけは知っておこう:寺本明,新井一,塩川芳昭,大畑建治(編) (2010)
  • Author(s): 齋藤清
  • Publisher: メディカルビュー,東京
• [Book] Anterior interhemispheric approach, anterior craniofacial approach.改訂第4版 脳神経外科臨床マニュアル:端和夫(編) (2010)
  • Author(s): 齋藤清
  • Publisher: シュプリンガー・ジャパン,東京
• [Book] 静脈洞に進展した髄膜腫の摘出術.脳腫瘍の外科Science, Art and Technology:原岡裏(編) (2010)
  • Author(s): 齋藤清, 伊藤英治, 佐藤拓, 安藤等, 松本由香, 市川優寛, 渡邉督, 佐久間潤, 永谷哲也, 若林俊彦
  • Publisher: 編集室なるにあ,東京
• [Book] NS NOW 3テント上髄膜腫(嗅溝髄膜腫) (2008)
  • Author(s): 齋藤清
  • Publisher: メディカルビュー
• [Book] 嗅溝髄膜腫. 「NS NOW 3 テント上髄膜腫 アプローチから摘出まで : 寺本明, 新井一, 塩川芳昭, 大畑建治(編)」 (2008)
  • Author(s): 齋藤清.
  • Publisher: メデイカルビュー, 東京.