a novel therapy of functioning pituitary adenomas using attenuated secretory machinery focusing porosome

• Project/Area Number: 20591723
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Teikyo University
• Principal Investigator: MATSUNO Akira Teikyo University, 医学部, 教授 (00242058)
• Co-Investigator(Renkei-kenkyūsha): MIZUTANI Akiko 帝京平成大学, 健康メディカル学部, 助教 (80465252)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 下垂体細胞 / 成長ホルモン / ポロソーム / 分泌機構 / 細胞内輸送 / VAMP / fodrin / porosome / GH / pituitary cell / siRNA / GH3 cell / AtT-20 cell / EYFP-GH

Research Abstract

Porosome was identified in an experimental rat pituitary cell expressing fluorophore-combined growth hormone. Both VAMP and fodrin were essential for the secretion of growth hormone. The suppression of these molecules induced the attenuated secretion of growth hormone. These results suggest that the suppression of VAMP and fodrin and the suppressed function of porosome will be a novel therapeutic target of growth hormone-producing pituitary adenomas.

Research Products

• [Journal Article] Skull base metastasis from follicular thyroid carcinoma: A report of two cases. (2010)
  • Author(s): Matsuno A, Katakami H, Okazaki R, Yamada S, Sasaki M, Nakaguchi H, Yamada SM, Hoya K, Murakami M, Yamazaki K, Ishida Y, Iwasaki H, Kuyama J, Kakudo K
  • Journal Title: Neurol Med Chir (Tokyo) 50 Pages: 421-425
  • Peer Reviewed
• [Journal Article] Small cell carcinoma originating from the cavernous sinus. (2010)
  • Author(s): Nakaguchi H, Matsuno A, Miyawaki S, Murakami M, Yamada M, Yamazaki K, Ishida Y
  • Journal Title: Acta Neurochir 152 Pages: 493-500
  • Peer Reviewed
• [Journal Article] the International FIPA consortium : Characterization of aryl hydrocarbon receptor interacting protein (AIP) mutations in familial isolated pituitary adenoma families. (2010)
  • Author(s): Igreja S, Chahal1 HS, King P, Bolger GB, Srirangalingam U, Guasti L, Chapple JP, Gueorguiev M, Guegan K, Stals K, Khoo B, Kumar AV, Ellard S, Grossman AB, Korbonits M
  • Journal Title: Hum Mutat 31 Pages: 1-11
  • Peer Reviewed
• [Journal Article] SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals. (2010)
  • Author(s): Asai-Akieda S, Zaima N, Ikegami K, Kahyo T, Yao I, Hatanaka T, Iemura S, Sugiyama R, Yokozeki T, Eishi Y, Koike M, Ikeda K, Chiba T, Yamaza H, Shimokawa I, Song SY,Matsuno A, Mizutani A, Sawabe M, Chao MV, Tanaka M, Kanaho Y, Natsume T, Sugimura H, Date Y, McBurney MW, Konioshi Y, Guarente L, Setou M
  • Journal Title: PLoS One 5
  • Peer Reviewed
• [Journal Article] A rare association of ruptured left middle cerebral artery aneurysm and dextrocardia with situs inversus totalis. (2010)
  • Author(s): Matsuno A, Miyawaki S, Yamada S, Ide F, Yamada SM, Nakaguchi H, Hoya K, Murakami M, Takeuchi M, Sugaya M
  • Journal Title: Ir J Med Sci Sep 25(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Current trends in the treatment of malignant pituitary tumors. (2010)
  • Author(s): Matsuno A, Murakami M, Mizutani A, Nakaguchi H, Yamada SM, Hoya K, Ide F, Yamada S, Onda Y, Takeuchi M, Okinaga H
  • Journal Title: Current Trends in Endocrinology 4 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Characterization of aryl hydrocarbon receptor interacting protein (AIP) mutations in familial isolated pituitary adenoma families (2010)
  • Author(s): Igreja S, ら
  • Journal Title: Hum Mutat Volume: 31 Pages: 1-11
  • Peer Reviewed
• [Journal Article] SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals (2010)
  • Author(s): Asai-Akieda S, ら
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] Current trends in the treatment of malignant pituitary tumors (2010)
  • Author(s): Matsuno A, ら
  • Journal Title: Current Trends in Endocrinology 2010, 4 : 1-7 Volume: 4 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Oral administration of cilnidipine to patients with hypertensive intracerebral hemorrhage in an acute stage : significance and role of an N-type calcium channel blocker. (2009)
  • Author(s): Matsuno A, Ide F, Tanaka H, Asano S, Miyawaki S, Uno T, Tanaka J, Nakaguchi H, Sasaki M, Murakami M, Fuke N
  • Journal Title: Ir J Med Sci 178 Pages: 419-422
  • Peer Reviewed
• [Journal Article] Preoperative elevated serum growth hormone is preventive of osteopenia in postsurgical patients with pituitary somatotroph adenomas : comparison with clinically non-functioning adenomas. (2009)
  • Author(s): Sasaki M, Okazaki R, Inoue D, Nakashima M, Ide F, Nakaguchi H, Yamada SM, Hoya K, Murakami M, Mizutani A, Okinaga H, Matsuno A
  • Journal Title: Endocrinologist 19 Pages: 208-210
  • Peer Reviewed
• [Journal Article] Analyses of factors influencing the acute effect of octreotide in growth hormone-secreting adenomas. (2009)
  • Author(s): Nakashima M, Takano K, Matsuno A
  • Journal Title: Endocr J 56 Pages: 295-304
  • NAID: 10026913199
  • Peer Reviewed
• [Journal Article] Molecular pathogenesis and functional analysis of pituitary adenoma. (2009)
  • Author(s): Matsuno A, Mizutani A, Takekoshi S, Osamura RY, Sanno N, Teramoto A, Ide F, Yamada S, Sasaki M, Nakaguchi H, Yamada SM, Hoya K, Murakami M, Okinaga H
  • Journal Title: Current Trends in Neurology 3 Pages: 69-73
  • Peer Reviewed
• [Journal Article] Oral administration of cilnidipine to patients with hypertensive intracerebral hemorrhage in anacute stage : significance and role_of an N-type calcium channel blocker (2009)
  • Author(s): Matsuno A, Ide F, Tanaka H, Asano S, Miyawaki S, Uno T, Tanaka, J, Nakaguchi H, Sasaki M, Murakami M, Fuke N
  • Journal Title: Ir J Med Sci 178 Pages: 419-422
  • Peer Reviewed
• [Journal Article] Preoperative elevated serum growth hormone is preventive of osteonenia in postsurgical patients with pituitary somatotroph adenomas : comparison with clinically non-functioning adenomas (2009)
  • Author(s): Sasaki M, Okazaki R, Inoue D, Nakashima, M, Ide F, Nakaguchi H, Yamada SM, Hoya K, Murakami M, Mizutani A, Okinaga H, Matsuno A
  • Journal Title: Endocrinologist 19 Pages: 208-210
  • Peer Reviewed
• [Journal Article] Analyses of factors_influencing the acute effect of octreotide in growth hormone-secreting adenomas (2009)
  • Author(s): Nakashima M, Takano K, Matsuno A
  • Journal Title: Endocr J 56 Pages: 295-304
  • Peer Reviewed
• [Journal Article] Disappearance of gadolinium enhancement in a chemoresistant astrocytoma of the tectum after high dose interferon beta. (2008)
  • Author(s): Matsuno A, Fujimaki T, Mizutani A, Ide F, Tanaka H, Asano S, Miyawaki S, Uno T, Tanaka J, Nakaguchi H, Sasaki M, Murakami M, Yamazaki K, Ishida Y
  • Journal Title: Tumori 94 Pages: 853-855
  • Peer Reviewed
• [Journal Article] Effective combined medical treatment with octreotide long acting release (Sandostatin LAR) and cabergoline for an extremely rare pituitary somatotroph adenoma producing growth hormone-releasing hormone (2008)
  • Author(s): Matsuno A, Katakami H, Okazaki R, Mizutani A, Osamura RY, Miyawaki S, Uno T, Ide F, Asano S, Tanaka J, Nakaguchi H, Sasaki M, Murakami M, Yamazaki K, Ishida Y, Okinaga H, Teramoto A
  • Journal Title: Endocrinologist 18 Pages: 266-269
  • Peer Reviewed
• [Journal Article] Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: a role of rab3B in secretion of GH through porosome. (2008)
  • Author(s): Matsuno A, Itoh J, Mizutani A, Takekoshi S, Osamura RY, Okinaga H, Ide F, Miyawaki S, Uno T, Asano S, Tanaka J, Nakaguchi H, Sasaki M, Murakami M
  • Journal Title: Folia Histochem Cytobiol 46 Pages: 419-421
  • Peer Reviewed
• [Journal Article] Prediction of the incidence of spontaneous intracerebral hemorrhage from meteorological data. (2008)
  • Author(s): Nakaguchi H, Matsuno A, Teraoka A
  • Journal Title: Int J Biometeorol 52 Pages: 323-329
  • Peer Reviewed
• [Journal Article] The role of the AIP gene in familial and sporadic pituitary adenomas. (2008)
  • Author(s): Leontiou CA, Gueorguiev M, van der Spuy J, Quinton R, Lolli F, Hassan S, Chahal HS, Igreja SC, Jordan S, Rowe J, Stolbrink M, Christian HC, Wray J, Bishop-Bailey D, Berney DM, Wass JAH, Popovic V, Ribeiro-Oliveira Jr. A, Gadelha MR, Monson JP, Davis JR, Clayton RN, Yoshimoto K, Iwata T, Matsuno A, Eguchi K, Musat M, Peters G, Bolger G, Chapple JP, Frohman LA, Grossman AB, Korbonits M
  • Journal Title: J Clin Endocrinol Metab 93 Pages: 2390-2401
  • Peer Reviewed
• [Journal Article] Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell : a role of rab3B in secretion of GH through porosome (2008)
  • Author(s): Matsuno A, et al.
  • Journal Title: Folia Histochem Cytobiol 46 Pages: 419-421
  • Peer Reviewed
• [Journal Article] Effective combined medical treatment with octreotide long acting release (Sandostatin LAR) and cabergoline for an extremely rare pituitary somatotroph adenoma producing growth hormone-releasing hormone (2008)
  • Author(s): Matsuno A, et al.
  • Journal Title: Endocrinologist 18 Pages: 266-269
  • Peer Reviewed
• [Journal Article] The role of the AIP gene in familial and sporadic pituitary adenomas (2008)
  • Author(s): Leontiou CA, et al.
  • Journal Title: J Clin Endocrinol Metab 93 Pages: 2390-2401
  • Peer Reviewed
• [Journal Article] Disappearance of gadolinium enhancement in a chemoresistant astrocytoma of the tectum after high dose interferon β (2008)
  • Author(s): Matsuno A, et al.
  • Journal Title: Tumori 2008 94 Pages: 853-855
  • Peer Reviewed
• [Journal Article] Functional molecular morphology of anterior pituitary cells, from hormone production to intracellular transport and secretion.
  • Author(s): Matsuno A, Mizutani A, Okinaga H, Takano K, Yamada S, Yamada SM, Nakaguchi H, Hoya K, Murakami M, Takeuchi M, Sugaya M, Itoh J, Takekoshi S, Osamura RY
  • Journal Title: Med Mol Morphol (in press)
  • NAID: 10029036664
  • Peer Reviewed
• [Journal Article] A mechanism of aquiring temozolomide resistance during transformation of atypical prolactinoma into prolactin-producing pituitary carcinoma
  • Author(s): Murakami M, Mizutani A, Asano S, Katakami H, Ozawa Y, Yamazaki K, Ishida Y, Takano K, Okinaga H, Matsuno A
  • Journal Title: Neurosurgery (in press)
  • Peer Reviewed
• [Journal Article] Combination therapy with rituximab and temozolomide for recurrent and refractory primary central nervous system lymphoma.
  • Author(s): Murakami M, Fujimaki T, Asano S, Nakaguchi H, Yamada SM, Hoya K, Yamazaki K, Ishida Y, Matsuno A
  • Journal Title: Yonsei Med J (in press)
  • Peer Reviewed
• [Journal Article] Abnormal dynamics of growth hormone after thyrotropin-releasing hormone loading test, gonadotropin-releasing hormone loading test, and oral glucose tolerance test in mixed gonadal dysgenesis with a pituitary lesion.
  • Author(s): Matsuno A, Yamada S, Yamada SM, Nakaguchi H, Hoya K, Murakami M
  • Journal Title: International Journal of Case Reports and Images (in press)
  • Peer Reviewed
• [Presentation] 下垂体細胞における下垂体ホルモンmRNAと蛋白の細胞内発現と細胞内動態、分泌機構の可視化 (2010)
  • Author(s): 松野彰
  • Organizer: 第42回日本臨床分子形態学会
  • Place of Presentation: 三島
  • Year and Date: 2010-09-25
• [Presentation] Microsurgery and neuroendoscopic surgery of pituitary tumors with medical and pathological studies. (2010)
  • Author(s): Matsuno A
  • Organizer: The 4th International Neurosurgical Conference
  • Place of Presentation: Harbin, China
  • Year and Date: 2010-07-17
• [Presentation] Microsurgery and neuroendoscopic surgery of pituitary tumors with medical and pathological studies (2010)
  • Author(s): Matsuno A
  • Organizer: The 4^<th> International Neurosurgical Conference
  • Place of Presentation: Harbin, China
  • Year and Date: 2010-07-17
• [Presentation] Temozolomide treatment of malignant pituitary tumors. (2010)
  • Author(s): Matsuno A, Murakami M, Mizutani A, Nakaguchi H, Yamada SM, Hoya K
  • Organizer: The 8th China-Japan Friendship Neurosurgical Symposium
  • Place of Presentation: Chengdu, China
  • Year and Date: 2010-06-18
• [Presentation] A case of subarachnoid with pituitary apoplexy caused by idiopathic pachymeningitis. (2010)
  • Author(s): Yamada SM, Yamada S, Onda Y, Nakaguchi H, Murakami M, Hoya K, Matsuno A, Nakayama H
  • Organizer: The 8th China-Japan Friendship Neurosurgical Symposium
  • Place of Presentation: Chengdu, China
  • Year and Date: 2010-06-18
• [Presentation] Temozolomide treatment of malignant pituitary tumors. (2010)
  • Author(s): Matsuno A, Murakami M, Mizutani A, Yamada S, Onda Y, Nakaguchi H, Yamada SM, Hoya K, Okinaga H
  • Organizer: The 2nd Russian-Japanese Neurosurgical Symposium
  • Place of Presentation: Yamanashi
  • Year and Date: 2010-05-10
• [Presentation] Differential diagnosis of adrenocorticotropin (ACTH) insufficiency by a novel ultrasensitive EIA. (2010)
  • Author(s): Katakami H, Hashida S, Matsuno A, Miyamura N, Usui T, Yamada S, Nishikawa T
  • Organizer: ICE2010
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-03-27
• [Presentation] Effectiveness and ineffectiveness of temozolomide for atypical prolactinoma and prolactin-producing pituitary carcinoma. (2010)
  • Author(s): Matsuno A, Mizutani A, Yamada S, Onda Y, Nakaguchi H, Yamada SM, Hoya K, Murakami M, Okinaga H, Ishibashi S
  • Organizer: Joint Neurosurgical Convention 2010
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2010-01-24
• [Presentation] Temozolomide is not effective for recurrent pituitary carcinoma transformed from atypical adenoma. (2010)
  • Author(s): Matsuno A, Murakami M, Mizutani A, Asano S, Ishida Y, Okinaga H
  • Organizer: 21^<st> Annual Meeting of Asaia-Pacific Endocrine Conference
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-01-10
• [Presentation] Temozolomide is not effective for recurrent pituitary carcinoma transformed from atypical adenoma. (2009)
  • Author(s): Matsuno A, Murakami M, Mizutani A, Asano S, Yamazaki K, Ishida Y, Okinaga H
  • Organizer: The 9th China-Japan Joint Seminar on Histochemistry and Cytochemistry
  • Place of Presentation: 南寧、中国
  • Year and Date: 2009-11-05
• [Presentation] Temozolomide is not effective for recurrent pituitary carcinoma transformed from atypical a denoma (2009)
  • Author(s): Matsuno A, Murakami M, Mizutani, A, Asano S, Ishida Y, Yamazaki K, Okinaga H
  • Organizer: The 9th China-Japan Joint Seminar on Histochemistry and Cytochemistry
  • Place of Presentation: Nannng, China
  • Year and Date: 2009-11-05
• [Presentation] Temozolomide is not effective for recurrent pituitary carcinoma transformed from atypical adenoma. (2009)
  • Author(s): Matsuno A, Murakami M, Mizutani A, Asano S, Ishida Y, Okinaga H
  • Organizer: The 11th International Pituitary Pathology Society (IPPS) Meeting
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2009-10-19
• [Presentation] Temozolomide is not effective for recurrent pituitary carcinoma transformed from atypical adenoma (2009)
  • Author(s): Matsuno A, Murakami M, Mizutani, A, Asano S, Ishida Y, Okinaga H
  • Organizer: The 11th International Pituitary Pathology Society (IPPS) Meeting
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2009-10-19
• [Presentation] Intravenous rituximab combined with temozolomide as a second line therapy for CD20 positive primary central nervous system malignant lymphoma. (2009)
  • Author(s): Matsuno A, Murakami M, Nakaguchi H, Sasaki M, Uno T, Ide F, Tanaka H, Fujimaki T
  • Organizer: The 3rd Quadrennial Meeting of the World Federation of Neuro-Oncology, jointly with The 6th Meeting of the Asian Society for Neuro-Oncology
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2009-05-14
• [Presentation] Ectopic acromegaly due to extracranial GHRH-producing tumors in Japan : Prevalence and clinical features. (2009)
  • Author(s): Katakami H, Genga S, Kawa S, Fujii S, Okajima T, Ishikwa E, , Nishina Y, Sugihara H, Kurita M, Hizuka N, Yamada S, Matsuno A, Hashida S
  • Organizer: The 11th International Pituitary Congress
  • Place of Presentation: Washinton D.C., U.S.A.
• [Presentation] In situ hybridization with quantum dot detection and confocal scanning microscopy for three-dimensional visualization of protein and mRNA : comparison with electron microscopic in situ hybridization. (2008)
  • Author(s): Matsuno A
  • Organizer: The 13th Congress of the International Federation of Societies for Histochemistry and Cytochemistry
  • Place of Presentation: Gdansk, Poland
  • Year and Date: 2008-08-27
• [Presentation] In situ hybridization with quantum dot detection and confocal scanning microscopy for three-dimensional visualization of protein and mRNA : comparison with electron microscopic in situ hybridization (2008)
  • Author(s): Matsuno A
  • Organizer: The 13th Congress of the International Federation of Societies for Histochemistry and Cytochemistry
  • Place of Presentation: Gdansk, Poland
  • Year and Date: 2008-08-27
• [Presentation] Contribution of factors influencing the acute effect of octreotide in GH-secreting adenomas. (2008)
  • Author(s): Matsuno A, Nakashima M, Takano K, Yasufuku-Takano J, Mizutani A, Osamura RY, Takei M, Tahara S, Teramoto A, Yamada M, Yamazaki K, Ishida Y, Katakami H, Ide F, Miyawaki S, Uno T, Tanaka J, Asano S, Nakaguchi H, Sasaki M, Murakami M
  • Organizer: 19th Annual Meeting of Asaia-Pacific Endocrine Conference
  • Place of Presentation: Shzhou, China
  • Year and Date: 2008-01-13
• [Book] Progress in Laser and Electro-Optics Research. (2010)
  • Author(s): Matsuno A, Mizutani A, Itoh J, Utsunomiya H, Takekoshi S, Osamura RY
  • Publisher: Nova Science Publishers, Inc.
• [Book] Antisense Elements (Genetics) Research Focus. (2008)
  • Author(s): Matsuno A
  • Publisher: Nova Science Publishers, Inc.
• [Book] Quantum Dots : Research, Technology and Applications. (2008)
  • Author(s): Matsuno A, Mizutani A, Takekoshi S, Osamura RY, Itoh J, Ide F, Miyawaki S, Uno T, Asano S, Tanaka J, Nakaguchi H, Sasaki M, Murakami M, Okinaga H
  • Publisher: Nova Science Publishers, Inc.
• [Book] New Research on Neurosecretory Systems. (2008)
  • Author(s): Matsuno A, Mizutani A, Takekoshi S, Osamura RY, Itoh J, Takano K, Ide F, Miyawaki S, Uno T, Asano S, Tanaka J, Nakaguchi H, Sasaki M, Murakami M, Okinaga H
  • Publisher: Nova Science Publishers, Inc.