Effects of bFGF-incorporated gelatin hydrogel and bone marrow-derived Schwann cell transplantation after spinal cord contusion injury in rats

• Project/Area Number: 20591736
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Chiba University
• Principal Investigator: OKAWA Akihiko Chiba University, 大学院・医学研究院, 講師 (30312945)
• Co-Investigator(Kenkyū-buntansha): YAMAZAKI Masashi 千葉大学, 大学院・医学研究院, 准教授 (50281712) ; MURATA Atsushi 千葉大学, 医学部附属病院, 准教授 (20344997)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: spinal cord injury / gelatin hydrogel / bFGF / allodynia / BBB score / BDA / 脊髄損傷 / ゼラチンゲル / 骨髄間質細胞 / 神経幹細胞 / BBBscore / シュワン細胞 / ラット / 徐放

Research Abstract

We investigated the effects of a sustained-release system of bFGF from gelatin hydrogel in a rat spinal cord contusion model.
One week after spinal cord contusion, gelatin hydrogel (GH) containing bFGF (20μg) was injected into the lesion epicenter of rats. Locomotor recovery was assessed over nine weeks by BBB testing. Sensory abnormalities in the hind paws of all the rats were evaluated at five, seven, and nine weeks.
Although BBB scores were better in the bFGF-GH and GH groups than in the saline group, the differences did not reach statistical significance. The bFGF-GH group showed significantly less sensory abnormalities than the saline group at seven weeks.
Our study findings suggest that bFGF-incorporated gelatin hydrogel could have therapeutic potential for alleviating mechanical allodynia following spinal cord injury.

Research Products

• [Journal Article] Transplantation of human bone marrow stromal cell-derived Schwann cells reduces cystic cavity and promotes functional recovery after contusion injury of adult rat spinal cord. (2011)
  • Author(s): Kamada T, Okawa A, Yamazaki M.
  • Journal Title: Neuropathology 31 Pages: 48-58
  • Peer Reviewed
• [Journal Article] Transplantation of human bone marrow stromal cell-derived Schwann cells reduces cystic cavity and promotes functional recovery after contusion injury of adult rat spinal cord. (2011)
  • Author(s): Kamada T, Okawa A, YamazakiM.
  • Journal Title: Neuropathology Volume: 31 Pages: 48-58
  • Peer Reviewed
• [Journal Article] Treatment of rat spinal cord injury with a Rho-kinase inhibitor 3 and bone marrow stromal cell transplantation (2009)
  • Author(s): 大河昭彦、村田淳、山崎正志
  • Journal Title: Brain Research 1295 Pages: 192-202
  • Peer Reviewed
• [Journal Article] Transplantation of Bone Marrow Stromal Cell-Derived Schwann Cell Reduces Cystic Cavity, Promotes Axonal…… (2008)
  • Author(s): Someya Y, Koda M, Dezawa M, et al.,
  • Journal Title: J Neurosurgery Spine 9(6) Pages: 600-610
• [Journal Article] Brain-derived neurotrophic factor suppresses anoikis-induced death of Schwann cells (2008)
  • Author(s): Koda M, Someya Y, Nishio Y, Kadota R, et al.,
  • Journal Title: Neuroscience Letter 444(2) Pages: 143-147
• [Journal Article] Transplantation of murine induced pluripotent stem (iPS) cell-derived astrocytes increases sensitivity to mechanical stimulus in a rat spinal cord injury model
  • Author(s): Hayashi K., Hashimoto M., Koda M., Naito A., Murata A., Okawa A., Takahashi K., Yamazaki Y.
  • Journal Title: J Neurosurgery Spine in press
  • Peer Reviewed
• [Presentation] ラット脊髄損傷に対するマウス人工多能性幹(iPS)細胞由来Astrocyte移植効果 (2010)
  • Author(s): 林浩一、山崎正志、大河昭彦、村田淳
  • Organizer: 日本整形外科基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-15
• [Presentation] EFFECTS OF BFGF INCORPORATED GELATIN HYDROGEL AND BONE MARROW STROMAL CELL-DERIVED NEURAL PROGENITOR CELL TRANSPLANTATION IN A RAT SPINAL CORD CONTUSION MODEL (2009)
  • Author(s): 大河昭彦、村田淳、山崎正志
  • Organizer: National Neurotrauma Society
  • Place of Presentation: Santa Barbara, CA, USA
  • Year and Date: 2009-09-09
• [Presentation] EFFECTS OF BFGF INCORPORATED GELATIN HYDROGEL AND BONE MARROW STROMAL CELLDERIVED NEURAL PROGENITOR CELL TRANSPLANTATION IN A RAT SPINAL CORD CONTUSION MODEL (2009)
  • Author(s): Hashimoto M., Furuya T., Koda M., Murata A., Okawa A., Yamazaki M., Dezawa M., Matsuse D., Tabata Y., Takahashi K.
  • Organizer: Neurotrauma
  • Place of Presentation: Santa Barbara, California
• [Presentation] ラット脊髄損傷に対するシロスタゾール投与の有用性の検討 (2008)
  • Author(s): 林浩一、國府田正雄、大河昭彦、村田淳、山崎正志
  • Organizer: 日本脊髄障害学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-11-06
• [Presentation] ラット脊髄圧挫損傷モデルにおけるbFGF徐放ゼラチンハイドロゲル移植の検討 (2008)
  • Author(s): 古矢丈雄, 橋本将行, 国府田正雄, 村田淳, 大河昭彦, 山崎正志, 出澤真理, 松瀬大, 田畑泰彦, 高橋和久
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-23
• [Presentation] Effects of bFGF incorporated gelatin hydrogel transplantation in a rat spinal cord contusion (2008)
  • Author(s): Furuya T., Hashimoto M., Koda M., Murata A., Okawa A., Yamazaki M., Dezawa M., Matsuse D., Tabata Y., Takahashi K.
  • Place of Presentation: Orlando, FL