| Project/Area Number |
20591748
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
MIYAKI Takayoshi (2010) Tokyo Medical University, 医学部, 准教授 (20157598)
宮木 孝昌 (2008-2009) Tokyo Medical University, 医学部, 准教授 (60366094)
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| Co-Investigator(Kenkyū-buntansha) |
TERAYAMA Hayato 東京医科大学, 医学部, 助教 (00384983)
ITOH Masahiro 東京医科大学, 医学部, 教授 (00232471)
NAITO Munekazu 東京医科大学, 医学部, 講師 (10384984)
HIRAI Shuichi 東京医科大学, 医学部, 助教 (70516054)
AOTA Yoichi 横浜市立大学, 医学部, 准教授 (40363824)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 脊椎脊髄病学(側彎症・整形外科・解剖学・組織学・内分泌) / 側彎 / 骨成長 / 動物モデル / 脊柱変性 / 骨成長速度 / 松果体 / 脊椎 / 側彎症 / カルセイン / ヒヨコ / メラトニン / テトラサイクリン |
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| Research Abstract |
Despite many studies over several decades, the etiology and pathogenesis of idiopathic scoliosis are not known. Melatonin, hormone produced by pineal gland, has been suggested to play an important role in scoliosis and osteoporosis development. In the present study, growth modulation and osteoporosis were analysed in mid-sagittal sections of T7 vertebral bodies in pinealectomy chicken model. Histomorphometric study was performed before the development of marked scoliosis (6 days after pinealectomy). All chickens were double labeled with Calcein injected at 2 and 4 days prior to sacrifice. In pinealectomy chickens, histologies detected a generalized osteopenia. This decrease in trabecular bone was particularly noticeable at the mid-portion of the vertebrae, where pinealectomy chickens had an approximately 50% lower BV/TV than control chickens (14.2±1.8 % versus 7.3±0.8 %; mean±s.d., n=5), which is highly significant (p<0.00005). Histomorphometric study revealed that pinealectomy induced high-turnover osteoporosis. Pinealectomy also resulted in substantial changes in endochondral ossification. In general, avian chondro-osseous junction undulates because metaphyseal vessels invade the growth plate at intervals, and growth plate cartilage becomes included within the newly formed bone. In normal chickens, about 9% of the chondro-osseous junction was covered by osteoblasts (Ob.S/BS). The coverage was significantly increased in pinealectomized chickens (9.1±4.3 % versus 38.9±11.1 %; p<0.05). Longitudinal growth rate, calculated based on unlabeled width in hypertrophic zone was significantly reduced (98.9±32.4 μm/day versus 45.8±5.5 μm/day, p<0.05). Previous in vitro study illustrated that melatonin inhibited the activity of both osteoblast and osteoclast in the co-culture of both cell types. This is the first report illustrating ongoing histological changes of impaired endochondral ossification in MLT deficiency.
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