Enhancement of cartilage differentiation of mesenchymal stem cells with Wnt protein stimulation

• Project/Area Number: 20591758
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Hiroshima University
• Principal Investigator: ADACHI Nobuo Hiroshima University, 病院, 准教授 (30294383)
• Co-Investigator(Kenkyū-buntansha): OCHI Mitsuo 広島大学, 病院, 教授 (70177244) ; 石川 正和 広島大学, 病院, 助教 (60372158)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 変形性関節症 / 再生医学 / Wnt / 関節軟骨

Research Abstract

Me senchymal stem cells (MSC) have been considered as good candidate as cell source in regenerative medicine. On the other hand, it is reported that Wnt protein can affect cartilage differentiation of those cells. This study clearly demonstrated that supplementation of Wnt5a protein enhanced cartilage differentiation of MSC in three-dimensional culture system.