Homing of circulating osteogenic progenitors to sites of fracture repair under the low-intensity pulsed ultrasound treatment

• Project/Area Number: 20591763
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Yokohama City University
• Principal Investigator: SAITO Tomoyuki Yokohama City University, 医学研究科, 教授 (30170517)
• Co-Investigator(Kenkyū-buntansha): KUMAGAI Ken 横浜市立大学, 医学部, 助教 (10468176)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: 骨折治癒 / 低出力超音波 / 末梢血由来細胞 / ホーミング / 骨形成促進効果 / 骨形成促進

Research Abstract

A parabiotic model was created by surgically conjoining one transgenic GFP mouse and one wild-type partner. After shared circulation was established, a transverse fracture with periosteal stripping was created in the contralateral diaphysial femur of the wild-type partner. The fracture site was treated with daily exposure of low-intensity pulsed ultrasound (LIPUS). GFP+ cells that gained access to the osteotomy site via the systemic circulation were identified in the bone-forming site. The findings suggested that LIPUS induced homing of circulating osteogenic progenitors to the osteotomy site for possible contribution to new bone formation.

Research Products

• [Journal Article] Engrafted VHL peptide-delivered bone marrow stromal cells promote spinal cord repair in rats. (2010)
  • Author(s): Yamazaki Y, Kanno H, Maeda K, Yoshida T, Kobayashi N, Kubo A, Yamaguchi Y, Saito T.
  • Journal Title: Neuroreport. 21(4) Pages: 287-292
  • Peer Reviewed
• [Journal Article] Efficient growth inhibition of human osteosarcoma cells using a peptide derived from the MDM-2-binding siisisi sif p53. (2010)
  • Author(s): Ito R, Kanno H, Takahashi A, Matsumoto R, Kobayashi N, Yoshida T, Saito T.
  • Journal Title: Protein Pept Lett. 17(5) Pages: 610-615
  • Peer Reviewed
• [Journal Article] Imaging of a rat osteoarthritis model using (18)F-fluoride positron emission tomography. (2010)
  • Author(s): Umemoto Y, Oka T, Inoue T, Saito T.
  • Journal Title: Ann Nucl Med. (Epub ahead of print)
  • NAID: 10027659532
  • Peer Reviewed
• [Journal Article] A mid term comparison of open wedge high tibial osteotomy vs unicompartmental knee arthroplasty for medial compartment osteoarthritis of the knee. (2010)
  • Author(s): Takeuchi R, Umemoto Y, Aratake M, Bito H, Saito I, Kumagai K, Sasaki Y, Akamatsu Y, Ishikawa H, Koshino T, Saito T.
  • Journal Title: J Orthop Surg Res. 5(1) Pages: 65-65
  • Peer Reviewed
• [Journal Article] In vitro stability of open wedge high tibial osteotomy with synthetic bone graft. (2010)
  • Author(s): Takeuchi R, Bito H, Akamatsu Y, Shiraishi T, Morishita S, Koshino T, Saito T.
  • Journal Title: Knee. 17(3) Pages: 217-220
  • Peer Reviewed
• [Journal Article] Opening wedge high tibial osteotomy affects both the lateral patellar tilt and patellar height. (2010)
  • Author(s): Bito H, Takeuchi R, Kumagai K, Aratake M, Saito I, Hayashi R, Sasaki Y, Saito T.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc. 18(7) Pages: 955-960
  • Peer Reviewed
• [Journal Article] 骨折治癒におけるcirculating osteogenic cellの関与 (2010)
  • Author(s): 熊谷研, 齋藤知行
  • Journal Title: 関節外科 Volume: 29 Pages: 749-751
• [Journal Article] Transplantation of Von Hippel-Lindau peptide delivered neural stem cells promotes recovery in the injured rat spinal cord. (2009)
  • Author(s): Maeda K, Kanno H, Yamazaki Y, Kubo A, Sato F, Yamaguchi Y, Saito T.
  • Journal Title: Neuroreport. 20(17) Pages: 1559-1563
  • Peer Reviewed
• [Journal Article] Relationship between low bone mineral density and varus deformity in postmenopausal women with knee osteoarthritis. (2009)
  • Author(s): Akamatsu Y, Mitsugi N, Taki N, Takeuchi R, Saito T.
  • Journal Title: J Rheumatol. 36(3) Pages: 592-597
  • Peer Reviewed
• [Journal Article] A predictive factor for acquiring an ideal lower limb realignment after opening-wedge high tibial osteotomy. (2009)
  • Author(s): Bito H, Takeuchi R, Kumagai K, Aratake M, Saito I, Hayashi R, Sasaki Y, Aota Y, Saito T.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc. 17(4) Pages: 382-389
  • Peer Reviewed
• [Journal Article] Clinical results and radiographical evaluation of opening wedge high tibial osteotomy for spontaneous osteonecrosis of the knee. (2009)
  • Author(s): Takeuchi R, Aratake M, Bito H, Saito I, Kumagai K, Hayashi R, Sasaki Y, Akamatsu Y, Ishikawa H, Amakado E, Aota Y, Saito T.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc. 17(4) Pages: 361-368
  • Peer Reviewed
• [Journal Article] Medial opening wedge high tibial osteotomy with early full weight bearing. (2009)
  • Author(s): Takeuchi R, Ishikawa H, Aratake M, Bito H, Saito I, Kumagai K, Akamatsu Y, Saito T.
  • Journal Title: Arthroscopy. 25(1) Pages: 46-53
  • Peer Reviewed
• [Journal Article] Simultaneous intraoperative detection of methicillin-resistant Staphylococcus and pan-bacterial infection during revision surgery : use of simple DNA release by ultrasonication and real-time polymerase chain reaction. (2009)
  • Author(s): Kobayashi N, Inaba Y, Choe H, Aoki C, Ike H, Ishida T, Iwamoto N, Yukizawa Y, Saito T.
  • Journal Title: J Bone Joint Surg Am. 91(12) Pages: 2896-2902
  • Peer Reviewed
• [Journal Article] Rapid and sensitive detection of methicillin-resistant Staphylococcus periprosthetic infections using real-time polymerase chain reaction. (2009)
  • Author(s): Kobayashi N, Inaba Y, Choe H, Iwamoto N, Ishida T, Yukizawa Y, Aoki C, Ike H, Saito T.
  • Journal Title: Diagn Microbiol Infect Dis. 64(2) Pages: 172-176
  • Peer Reviewed
• [Journal Article] Evaluation of lesion in a spontaneous osteonecrosis of the knee using 18F-fluoride positron emission tomography. (2009)
  • Author(s): Aratake M, Yoshifumi T, Takahashi A, Takeuchi R, Inoue T, Saito T.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc. 17(1) Pages: 53-59
  • Peer Reviewed
• [Journal Article] Changes in physical function after palliative surgery for metastatic spinal tumor : association of the revised Tokuhashi score with neurologic recovery. (2008)
  • Author(s): Yamashita T, Aota Y, Kushida K, Murayama H, Hiruma T, Takeyama M, Iwamura Y, Saito T.
  • Journal Title: Spine. 33(21) Pages: 2341-2346
  • Peer Reviewed
• [Journal Article] Clinical results of a valgus high tibial osteotomy for the treatment of osteoarthritis of the knee and the ipsilateral ankle. (2008)
  • Author(s): Takeuchi R, Saito T, Koshino T.
  • Journal Title: Knee. 15(3) Pages: 196-200
  • Peer Reviewed
• [Journal Article] Circulating cells with osteogenic potential are physiologically mobilized into the fracture healing site in the parabiotic mice model. (2008)
  • Author(s): Kumagai K, Vasanji A, Drazba JA, Butler RS, Muschler GF.
  • Journal Title: J Orthop Res. 26(2) Pages: 165-175
  • Peer Reviewed
• [Journal Article] Cellular strategies for enhancement of fracture repair. (2008)
  • Author(s): Patterson TE, Kumagai K, Griffith L, Muschler GF.
  • Journal Title: J Bone Joint Surg Am. 90 Suppl 1 Pages: 111-119
  • Peer Reviewed
• [Presentation] マウス骨切りモデルにおける低出力超音波パルス(LIPUS)刺激の骨形成促進効果と末梢血由来骨前駆細胞の関与 (2010)
  • Author(s): 熊谷研
  • Organizer: 第13回超音波骨折治療研究会
  • Place of Presentation: 東京 東京ステーションコンファレンス
  • Year and Date: 2010-01-23
• [Presentation] Bone formation and homing of circulating connective tissue progenitors at fracture site were enhanced by low-intensity pulsed ultrasound. (2010)
  • Author(s): Kumagai K, Takeuchi R, Ishikawa H, Yamaguchi Y, Fujisawa T, Kuniya T, Takagawa S, Saito T
  • Organizer: 7th Combined Meeting of the Orthopaedic Research Societies
  • Place of Presentation: Kyoto
• [Presentation] Recruitment of recipient derived osteogenic connective tissue progenitors to β-tricalcium phosphate scaffold combined with bone marrow cells. (2010)
  • Author(s): Kumagai K, Takeuchi R, Ishikawa H, Saito I, Yamaguchi Y, Chiba N, Takagawa S, Saito T
  • Organizer: 56th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans
• [Presentation] Low-Intensity Pulsed Ultrasound Enhances Fracture Healing and Recruitment of Circulating Osteogenic Progenitors. (2010)
  • Author(s): 熊谷研
  • Organizer: The 20th Japanese-Korean Combined Orthopaedic Symposium
  • Place of Presentation: 鹿児島 指宿 白水館
• [Presentation] 骨膜除去骨折モデルへの低出力超音波刺激による骨形成促進と末梢血由来骨前駆細胞の関与 (2009)
  • Author(s): 熊谷研, 竹内良平, 荒武正人, 斎藤泉, 尾藤晴彦, 林陸, 佐々木洋平, 齋藤知行
  • Organizer: 第24回日本整形外科学会基礎学術集会
  • Place of Presentation: 横浜
• [Presentation] Effects of Low-Intensity Pulsed Ultrasound on Intramedullary Bone Formation and Homing of Circulating Connective Tissue Progenitors in Fracture with Periosteal Stripping. (2009)
  • Author(s): Kumagai K, Takeuchi R, Ishikawa H, Saito I, Yamaguchi Y, Chiba N, Takagawa S, Saito T
  • Organizer: 31st Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Denver, CO
• [Presentation] Low-intensity pulsed ultrasound stimulates iIntramedullary bone formation and homing of circulating connective tissue progenitors at fracture site with periosteal stripping in mice. (2009)
  • Author(s): 熊谷研, 竹内良平, 荒武正人, 斎藤泉, 尾藤晴彦, 佐々木洋平, 齋藤知行
  • Organizer: 55th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Las Vegas, NV
• [Presentation] Effects of Low-Intensity Pulsed Ultrasound on Intramedullary Bone Formation and Homing of Circulating Connective Tissue Progenitors in Fracture with Periosteal Stripping (2009)
  • Author(s): 熊谷研
  • Organizer: 31st Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Denver, CO, USA
• [Presentation] Low-Intensity Pulsed Ultrasound Stimulates Intramedullary Bone Formation and Homing of Circulating Connective Tissue Progenitors at Fracture Site with Periosteal Stripping in Mice. (2009)
  • Author(s): 熊谷 研
  • Organizer: 55th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Las Vegas, NV, USA