| Project/Area Number |
20591765
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
HONOKI Kanya Nara Medical University, 医学部・医学科, 講師 (40336863)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIUCHI Toshifumi 近畿大学, 理工学部・生命科学科, 教授 (10254492)
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| Research Collaborator |
TANAKA Yasuhito 奈良県立医科大学, 医学部・医学科, 教授 (30316070)
TOHMA Yasuaki 奈良県立医科大学, 医学部・医学科, 博士研究員 (60448777)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | sarcoma / cancer stem cell / gene expression / drug resistance / osteosarcoma / mesenchymal stem cell / metastasis / stem cell niche / sarcosphere / DNA repair / INK4a |
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| Research Abstract |
The current study indicates that rat and human sarcoma cells contain subpopulation of stem like cells with the formation of 'sarcoshpere'in anchorage-independent, serum starved culture condition, showing higher expression of stemness genes with the ability of self-renewal. Sarcosphere cells show the capacity of resistance to chemo-agents due to the up-regulation of DNA repair enzymes. These results suggest that the chemo-resistance in sarcomas might be caused in part by the presence of stem-like cell populations. These results suggest that targeting sarcoma stem cells could improve the prognosis of sarcoma patients.
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