New methods of differential diagnosis and treatment by GABA system in human cartilage tumors
| Project/Area Number |
20591768
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
KANBARA Kiyoto Osaka Medical College, 医学部, 講師 (40298758)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Yoshiaki 大阪医科大学, 医学部, 准教授 (70268192)
渡辺 正仁 大阪医科大学, 医学部, 准教授 (70084902)
相馬 義郎 慶應義塾大学, 医学部, 准教授 (60268183)
植田 直樹 大阪医科大学, 医学部, 助教 (70298751)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | GABA / GABA受容体 / 軟骨肉腫 / OUMS-27 / アポトーシス / GABA_B受容体 / GAD / Caチャネル / GABA B受容体 / HCS2/8 / real time PCR / パッチクランプ |
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| Research Abstract |
We investigated GABA system in human cartilage tumors to establish new methods of differential diagnosis and treatment. We detected strongly GAD65 mRNA in human cartilage tumors and chondrosarcoma cells using real time PCR. BrdU incorporation of the cells treated with GABA and GABA receptor agonist was significantly increased, while that of the cells treated with GABA receptor antagonist was significantly inhibited (p>0.01). Our findings indicate that GABA system is related to the proliferation process in human cartilage tumors.
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Report
(4 results)
Research Products
(9 results)
