Development of target therapy for SSX of sarcoma metastasis

• Project/Area Number: 20591771
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses
• Principal Investigator: YOSHIOKA Kiyoko Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses, 研究所, 研究員 (40342993)
• Co-Investigator(Kenkyū-buntansha): ITOH Kazuyuki 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), 研究所, 部長 (20301806)
• Co-Investigator(Renkei-kenkyūsha): YOSHIKAWA Hideki 大阪大学, 大学院・医学系研究科器官制御外科学, 教授 (60191558)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: SSX / 骨軟部腫瘍 / 浸潤 / 転移 / lung metastasis / invasion / MMP-1 / siRNA / LC/MS/MS / ChIP / LC / MS / osteosarcoma

Research Abstract

The SSX genes were initially identified as fusion partners to the SS18 gene in synovial sarcoma. Besides adult human testis, SSX genes were highly expressed in sarcoma, and positively correlated with clinical stage, especially the metastatic case present very high SSX expression. Our results suggested that SSXs would be novel proteins regulate cancer metastasis and potential molecular target(s) under clinical setting.

Research Products

• [Journal Article] Suppression of Colon Cancer Metastasis by Aes through Inhibition of Notch Signaling (2011)
  • Author(s): Sonoshita, M., Aoki, M., Fuwa, H., Aoki K., Hosogi, H., Sakai, Y., Hashida, H., Takabayashi, A., Sasaki, M., Robine, S., Itoh K., Yoshioka K., Kakizaki, F., Kitamura, T., Oshima, M., Taketo. M.M.
  • Journal Title: Cancer Cell 19 Pages: 125-137
  • Peer Reviewed
• [Journal Article] Suppression of Colon Cancer Metastasis by Aes through Inhibition of Notch Signaling (2011)
  • Author(s): Sonoshita, Masahiro
  • Journal Title: Cancer Cell Volume: 19 Pages: 125-137
  • Peer Reviewed
• [Journal Article] 骨・軟部腫瘍の転移に関与する新規標的分子SSX (2010)
  • Author(s): 吉岡潔子、伊藤和幸、中紀文、荒木信人、竹中聡
  • Journal Title: 整形・災害外科 53巻、No.12 Pages: 1344-1345
• [Journal Article] Mesenchymal mode of migration participates in pulmonary metastasis of mouse osteosarcoma LM8. (2010)
  • Author(s): Yui, Y., Itoh K., Yoshioka K., Naka N., Watanabe, M., Hiraumi, Y., Matsubara, H., Watanabe, K., Sano, K., Nakahata, T., Adachi, S.
  • Journal Title: Clin Exp Metastasis 27 Pages: 619-630
  • Peer Reviewed
• [Journal Article] Synovial Sarcoma Is a Stem Cell Malignancy. (2010)
  • Author(s): Naka N., Takenaka S., Araki N., Miwa, T., Hashimoto, N., Yoshioka K., Joyama, S., Hamada, K., Tsukamoto, Y., Tomita, Y., Ueda, T., Yoshikawa, H., Itoh K.
  • Journal Title: Stem Cells 28 Pages: 1119-1131
  • Peer Reviewed
• [Journal Article] Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo. (2010)
  • Author(s): Takenaka S., Naka N., Araki N., Hashimoto, N., Ueda, T., Yoshioka K.,, Yoshikawa, H., Itoh K.
  • Journal Title: Int.J.Oncology 36(4) Pages: 823-831
  • Peer Reviewed
• [Journal Article] SSX(synovial sarcoma X chromosome breakpoint)遺伝子 (2010)
  • Author(s): 吉岡潔子、中紀文、竹中聡、伊藤和幸
  • Journal Title: 臨床整形外科 45巻、Vol.2 Pages: 168-171
• [Journal Article] 骨・軟部腫瘍の転移に関与する新規標的分子SSX (2010)
  • Author(s): 吉岡潔子
  • Journal Title: 整形・災害外科 Volume: 53(12) Pages: 1344-1345
• [Journal Article] Synovial Sarcoma Is a Stem Cell Malignancy (2010)
  • Author(s): Naka, Norifumi
  • Journal Title: Stem Cells Volume: 28 Pages: 1119-1131
  • Peer Reviewed
• [Journal Article] Mesenchymal mode of migration participates in pulmonary metastasis of mouse osteosarcoma LM8 (2010)
  • Author(s): Yui, Yoshihiro
  • Journal Title: Clin Exp Metastasis Volume: 27 Pages: 619-630
  • Peer Reviewed
• [Journal Article] Synovial sarcoma is a stem cell malignancy (2010)
  • Author(s): Naka, Norifumi
  • Journal Title: Stem Cells (in press)
  • Peer Reviewed
• [Journal Article] Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo (2010)
  • Author(s): Takenaka, Satoshi
  • Journal Title: Int J Oncology 36 Pages: 823-831
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 吉岡潔子
  • Journal Title: 臨床整形外科(第42巻 第2号)最新基礎科学/知っておきたいSSX(synovial sarcoma X chromosome breakpoint)遺伝子(医学書院) Pages: 168-171
• [Journal Article] IGF-I secreted by osteoblasts acts as a potent chemotactic factor for osteoblasts. (2008)
  • Author(s): Nakasaki, M., Yoshioka K., Miyamoto, Y., Sasaki, T., Yoshikawa, H., Itoh K.
  • Journal Title: Bone 43 Pages: 869-879
  • Peer Reviewed
• [Journal Article] Evidence for efficient phosphorylation of EGFR and rapid endocytosis of phosphorylated EGFR via the early/late endocytic pathway in a gefitinib-sensitive non-small cell lung cancer cell line. (2008)
  • Author(s): Nishimura, Y., Yoshioka K., Bereczky, B., Itoh K.
  • Journal Title: Molecular Cancer 7(42) Pages: 1-13
  • Peer Reviewed
• [Journal Article] Evidence for efficient phosphorylation of EGFR and rapid endocytosis of phosphorylated EGFR via the early/late endocytic pathway in a gefitinib-sensitive non-small cell lung cancer cell line (2008)
  • Author(s): Nishimura, Yukio
  • Journal Title: Molecular Cancer 7:42 Pages: 1-13
  • Peer Reviewed
• [Journal Article] IGF-I secreted by osteoblasts acts as a potent chemotactic factor for osteoblasts (2008)
  • Author(s): Nakasaki, Manando
  • Journal Title: Bone 43 Pages: 869-879
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): 吉岡潔子(日本がん転移学会編)
  • Journal Title: がん転移研究の実験手法(金芳堂) Pages: 195-200
• [Presentation] SSXの浸潤・転移におけるRac1-myosinの経路とMMP-1の関与 (2010)
  • Author(s): 吉岡潔子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: リーガロイヤルホテル大阪(大阪府)
  • Year and Date: 2010-09-22
• [Presentation] SSXの浸潤・転移におけるRac-1-myosinの経路とMMP-1の関与 (2010)
  • Author(s): 吉岡潔子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: リーガロイヤルホテル大阪(大阪府)
  • Year and Date: 2010-09-22
• [Presentation] 骨・軟部腫瘍の肺転移に関与する分子標的SSX (2010)
  • Author(s): 吉岡潔子
  • Organizer: 第43回日本整形外科学会 骨・軟部腫瘍学術集会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-07-15
• [Presentation] 骨・軟部腫瘍の肺転移に関与する分子標的SSX (2010)
  • Author(s): 吉岡潔子
  • Organizer: 第43回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-07-15
• [Presentation] ヒストン脱アセチル化酵素阻害剤・酪酸ナトリウムによるがん細胞浸潤の抑制 (2010)
  • Author(s): 吉岡潔子
  • Organizer: 第19回日本がん転移学会学術集会・総会
  • Place of Presentation: 金沢市文化ホール(石川県)
  • Year and Date: 2010-06-16
• [Presentation] 骨・軟部腫瘍の肺転移に対する分子標的SSXの機能解析 (2009)
  • Author(s): 吉岡潔子
  • Organizer: 第24回日本整形外科学会基礎学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-11-06
• [Presentation] 骨・軟部腫瘍の肺転移に対する分子標的SSXの機能解析 (2009)
  • Author(s): 吉岡潔子
  • Organizer: 第24回日本整形外科学会基礎学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-11-06
• [Presentation] SSXの浸潤・転移におけるPARP-1、Histone H1、MMP-1の関与 (2009)
  • Author(s): 吉岡潔子
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-10-01
• [Presentation] SSXの浸潤・転移におけるPARP-1、Histone H1、MMP1の関与 (2009)
  • Author(s): 吉岡潔子
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Presentation] 骨軟部腫瘍の肺転移に対する分子標的SSXの機能解析 (2009)
  • Author(s): 吉岡潔子
  • Organizer: 第18回日本がん転移学会 学術集会・総会
  • Place of Presentation: 旭川グランドホテル(北海道)
  • Year and Date: 2009-07-24
• [Presentation] 骨軟部腫瘍の肺転移に対する分子標的SSXの機能解析 (2009)
  • Author(s): 吉岡潔子
  • Organizer: 第18回日本がん転移学会学術集会・総会
  • Place of Presentation: 旭川
  • Year and Date: 2009-07-24
• [Presentation] 骨・軟部腫瘍の肺転移に対する分子標的SSXの機能解析 (2009)
  • Author(s): 吉岡潔子
  • Organizer: 第42回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-07-16
• [Presentation] 骨・軟部腫瘍の肺転移に対する分子標的SSXの機能解析 (2009)
  • Author(s): 吉岡潔子
  • Organizer: 第42回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-07-16
• [Presentation] SSX regulate cancer invasion via PARP-1, Histone H1 and MMP-1 signaling. (2008)
  • Author(s): Itoh, Kazuyuki
  • Organizer: The 48th American Society for Cell Biology Annual Meeting
  • Place of Presentation: Moscone Center, San Francisco, U.S.A
  • Year and Date: 2008-12-16
• [Presentation] SSX regulate cancer invasion via PARP-1, Histone H1 and MMP1 signaling (2008)
  • Author(s): Itoh, Kazuyuki
  • Organizer: The 48th American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, U. S. A
  • Year and Date: 2008-12-16
• [Presentation] 骨・軟部腫瘍の肺転移に関与する分子SSXの機能解析 (2008)
  • Author(s): 吉岡潔子
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2008-10-29
• [Presentation] 骨・軟部腫瘍の肺転移に関与する分子SSXの機能解析 (2008)
  • Author(s): 吉岡潔子
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] 骨・軟部腫瘍の肺転移に関与する分子SSXの機能解析 (2008)
  • Author(s): 吉岡潔子
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2008-10-24
• [Presentation] 骨・軟部腫瘍の肺転移に関与する分子SSXの機能解析 (2008)
  • Author(s): 吉岡潔子
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-24
• [Presentation] A novel highly metastatic synovial sarcoma cell line, Yamato-SS, is enriched cancer stem cells. (2008)
  • Author(s): Naka, Norifumi
  • Organizer: Joint Metastasis Research Society-AACR Conference on Metastasis
  • Place of Presentation: The Fairmont Hotel Vancouver, Vancouver, Canada
  • Year and Date: 2008-08-05
• [Presentation] A novel highly metastatic synovial sarcoma cell line, Yamato -SS, is enriched cancer stem cells (2008)
  • Author(s): Naka, Norifumi
  • Organizer: Joint Metastasis Research Society-AACR Conference on Metastasis
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2008-08-05
• [Presentation] SSX-New Molecular target Regulating Cancer Invasion and Metastasis. (2008)
  • Author(s): Itoh, Kazuyuki
  • Organizer: Joint Metastasis Research Society-AACR Conference on Metastasis
  • Place of Presentation: The Fairmont Hotel Vancouver, Vancouver, Canada
  • Year and Date: 2008-08-04
• [Presentation] SSX-New Molecular target Regulating Cancer Invasion and Metastasis (2008)
  • Author(s): Itoh, Kazuyuki
  • Organizer: Joint Metastasis Research Society-AACR Conference on Metastasis
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2008-08-04
• [Presentation] 骨・軟部腫瘍の肺転移に関与する分子SSXの機能解析 (2008)
  • Author(s): 吉岡潔子
  • Organizer: 第17回日本がん転移学会総会
  • Place of Presentation: 鹿児島サンロイヤルホテル(鹿児島県)
  • Year and Date: 2008-07-24
• [Presentation] 骨・軟部腫瘍の肺転移に関与する分子SSXの機能解析 (2008)
  • Author(s): 吉岡潔子
  • Organizer: 第17回日本がん転移学会総会
  • Place of Presentation: 鹿児島
  • Year and Date: 2008-07-24
• [Presentation] 骨・軟部腫瘍の転移に対する新規分子標的SSXの機能解析 (2008)
  • Author(s): 吉岡潔子
  • Organizer: 第41回整形外科学会 骨・軟部腫瘍学術集会
  • Place of Presentation: アクトシティ浜松(静岡県)
  • Year and Date: 2008-07-18
• [Presentation] 骨・軟部腫瘍の転移に対する新規分子標的SSXの機能解析 (2008)
  • Author(s): 吉岡潔子
  • Organizer: 第41回整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2008-07-18
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