| Project/Area Number |
20591777
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
HASHIMOTO Jun (2010) Osaka University, 医学系研究科, 准教授 (40237938)
南平 昭豪 (2008-2009) Osaka University, 医学系研究科, 助教 (70464245)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Hideki 大阪大学, 医学系研究科, 教授 (60191558)
MYOUI Akira 大阪大学, 医学部附属病院, 准教授 (10263261)
SHI Kenrin 大阪大学, 医学系研究科, 助教 (60575893)
HIRAO Makoto 大阪大学, 医学系研究科, 助教 (10527708)
橋本 淳 大阪大学, 医学系研究科, 准教授 (40237938)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 骨・軟骨代謝学 / 低酸素濃度 / 骨形成 / 軟骨分化 / 低酸素 / Nkx3.2 / 転写因子 / 破骨細胞 / 高酸素濃度 / 酸化ストレス / M-CSF / 生存 |
|---|
| Research Abstract |
We showed that a high oxygen tension markedly prolonged the duration of osteoclast precursor formation in the presence of supporting cells, and also markedly and persistently increased the production of macrophage colony stimulating factor (M-CSF) by supporting cells. These findings could explain some part of pathophysiology in severe bone destruction or osteolysis accompanied with abundant blood vessel and numeraous osteoclasts seen in several pathological conditions such as rheumatoid arthritis (RA) and primary or metastatic tumors. We also showed that O_2 concentrations decreased depending of the depth in both 20 and 5% O_2 tension, so medium depth is one of critical conditions to study the relation of the cellular environment of pericellular oxygen tension and cell biology.
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