Elucidation of the mechanism involved in ischemic preconditioning using siRNA technology

• Project/Area Number: 20591803
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Osaka University
• Principal Investigator: KAMIBAYASHI Takahiko Osaka University, 医学系研究科, 助教 (10273640)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Yukio 大阪大学, 医学系研究科, 准教授 (60294063) ; SHIBUTA Satoshi 大阪大学, 医学系研究科, 助教 (20324767)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ラット / プレコンディショニング / 脳虚血 / 心筋虚血 / 脳死 / 心機能

Research Abstract

We tried to elucidate the role of hypoxia inducible factor (HIF)-1α in ischemic preconditioning by regulating HIF-1α gene expression using siRNA technology, but the effect of the siRNA did not reach statistical significance. During the experiment, we found that brain damage significantly affected cardiac function, and that the mitochondrial ATP sensitive potassium channel, which is known to be another important component of preconditioning, played an important role in the phenomenon.

Research Products

• [Journal Article] Nicorandil preserves myocardial function following brain death in rats by mitochondrial adenosine triphosphate- sensitive potassium channel-dependent mechanism. (2011)
  • Author(s): Mitsuo Iwasaki, Yukio Hayashi, Hiroo Yamanaka, Takahiko Kamibayashi, Takashi Mashimo
  • Journal Title: Eur J Cardiothorac Surg(電子出版済) 印刷中
  • Peer Reviewed
• [Journal Article] Nicorandil preserves myocardial function following brain death in rats by mitochondrial adenosine triphosphate-sensitive potassium channel-dependent mechanism (2011)
  • Author(s): Iwasaki K, Kamibayashi T
  • Journal Title: Eur J Cardiothorac Surg Volume: (印刷中 電子出版済)
  • Peer Reviewed