| Project/Area Number |
20591807
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Oita University |
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Principal Investigator |
KITANO Takaaki Oita University, 医学部, 教授 (20211196)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUMARU Osamu 大分大学, 医学部, 准教授 (40360151)
YOKOI Isao 大分大学, 医学部, 教授 (80150366)
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| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 病態生理学 / 核磁気共鳴法 / 脳虚血 / 脳保護 / 電子スピン共鳴法 / フルクトース1,6-ニリン酸 / クレアチンリン酸 / ATP / フルクトース1,6-2リン酸 / 虚血-再灌流 / fructose-1, 6-dihoshate / 脱分極 / エネルギー代謝 / 高エネルギーリン酸 / fructose-1,6-diphosphate |
|---|
| Research Abstract |
Fructose 1,6-diphophate is an intermediate substrate of glycolysis. It is a known neuroprotectant and acts as energy metabolite and a radical scavenger. The neuroprotective capacity of FDP was studied using 31P-nuclear magnetic resonance spectroscopy (^<31>P-NMR). The state of energy metabolism during ischemia-reperfusion insult was evaluated by 31P-NMR. The recovery of phosphocreatine, energetic buffer of ATP in living cells, was significantly higher when brain slices were superfused with 5 mM FDP. The series of our experiments, including 31P-NMR and electron spin resonance spectroscopy, indicated that FDP functions as a neuroprotectant not only as an energy metabolite of glycolysis but also as a radical scavenger.
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