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The study of a new target for the effects of volatile anesthetics by immunoblotting.

Research Project

Project/Area Number 20591817
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionTokyo Medical University

Principal Investigator

SEKINE Syusuke (2009-2010)  Tokyo Medical University, 医学部, 助教 (10384976)

松本 晶平 (2008)  Tokyo Medical University, 医学部, 准教授 (30256250)

Co-Investigator(Kenkyū-buntansha) 関根 秀介  東京医科大学, 医学部, 助教 (10384976)
Co-Investigator(Renkei-kenkyūsha) MUROZONO Mitihiro  東京医科大学, 医学部, 講師 (70276947)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsイソフルラン / セボフルラン / プロポフォール / PKC-γ / リン酸化PKC-γ / PKCγ / リン酸化PKCγ / イソフルレン / セボフルレン
Research Abstract

Aims : We examined the effects of different types of anesthetics on PKC-γ knockout mice. And we investigated the dynamics of the kinase in brain cells obtained from mice anesthetized with volatile and i.v anesthetics.
Method and Results:We measured the required times for loss of righting reflex (rtfLORR) after administration of isoflurane, sevoflurane, or propofol on PKC-γ knockout mice and compared the times with those of wild-type mice. Volatile anesthetics (isoflurane and sevoflurane) significantly prolonged the rtfLORRs in PKC-γ knockout mice compared to those in wild-type mice. On the other hand, no significant difference was observed between knockout and wild-type mice treated with propofol . We also used immunoblotting to investigate the intracellular distribution of PKC-γ and phosphorylated PKC-γ (p-PKC-γ) in brain cell fractions obtained from wild-type mice during the LORR induced by these anesthetics. PKC-γ was significantly decreased in the synaptic membrane fraction (P2), whereas p-PKC-γ was significantly increased in P2. There was no significant change in the supernatant fraction (S). In propofol-treated mice, PKC-γ and p-PKC-γ showed no significant changes in P2 or S.
Conclusion : Our results provide new evidence to support the possibility of the involvement of PKC-γ in the actions of volatile anesthetics.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (7 results)

All 2011 2009 2008

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (3 results)

  • [Journal Article] Effects of different types of aneathetic arents on cellular PKC γ dynamics in mouse brain2011

    • Author(s)
      Akiko Takeda, Ryoichi Miyashita, Takeo Nagura, Shusuke Sekine, Michihiro Murozono, Shohei Matsumoto, Hiroyuki Uchino
    • Journal Title

      Pharmacology 87巻

      Pages: 180-186

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of different types of aneathetic arents on cellular PKCγ dynamics in mouse brain2011

    • Author(s)
      Akiko Takeda
    • Journal Title

      Pharmacology

      Volume: 87 Pages: 180-186

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isoflurane inhibits protein kinase C gamma and calcium/calmoduline dependent protein kinase II-alfa translocation to synaptic membrane in ischemic mice brains.2008

    • Author(s)
      Shohei Matsumoto, Michihiro Murozono, Daisuke Nagaoka, Shuhei Matsuoka, Akiko Takeda, Hideyuki Narita, Seigo Watanabe, Atsushi Isshiki, Yasuo Watanabe
    • Journal Title

      Neurochemical Res 33巻

      Pages: 2302-9

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isoflurane inhibits protein kinase C gamma and calcium/calumoduline dependent protein kinase II-alfa translocation to synaptic membrane in ischemic mice brains.2008

    • Author(s)
      Matsumoto S
    • Journal Title

      Neurochem. Res. 33

      Pages: 2302-9

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] プロテインキナーゼC-γノックアウトマウスを用いた吸入麻酔薬作用機序の検討2009

    • Author(s)
      宮下亮一
    • Organizer
      第56回日本麻酔科学学術集会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-08-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] プロテインキナーゼCγノックアウトマウスを用いた吸入麻酔薬作用機序の検討2009

    • Author(s)
      宮下亮一
    • Organizer
      日本麻酔科学会第56回学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2009-08-16
    • Related Report
      2010 Final Research Report
  • [Presentation] 吸入麻酔薬によるプロテインキナーゼCγの脳細胞内再分布2008

    • Author(s)
      武田明子
    • Organizer
      日本麻酔科学会第55回学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2008-06-14
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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