| Project/Area Number |
20591817
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
SEKINE Syusuke (2009-2010) Tokyo Medical University, 医学部, 助教 (10384976)
松本 晶平 (2008) Tokyo Medical University, 医学部, 准教授 (30256250)
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| Co-Investigator(Kenkyū-buntansha) |
関根 秀介 東京医科大学, 医学部, 助教 (10384976)
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| Co-Investigator(Renkei-kenkyūsha) |
MUROZONO Mitihiro 東京医科大学, 医学部, 講師 (70276947)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | イソフルラン / セボフルラン / プロポフォール / PKC-γ / リン酸化PKC-γ / PKCγ / リン酸化PKCγ / イソフルレン / セボフルレン |
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| Research Abstract |
Aims : We examined the effects of different types of anesthetics on PKC-γ knockout mice. And we investigated the dynamics of the kinase in brain cells obtained from mice anesthetized with volatile and i.v anesthetics.
Method and Results:We measured the required times for loss of righting reflex (rtfLORR) after administration of isoflurane, sevoflurane, or propofol on PKC-γ knockout mice and compared the times with those of wild-type mice. Volatile anesthetics (isoflurane and sevoflurane) significantly prolonged the rtfLORRs in PKC-γ knockout mice compared to those in wild-type mice. On the other hand, no significant difference was observed between knockout and wild-type mice treated with propofol . We also used immunoblotting to investigate the intracellular distribution of PKC-γ and phosphorylated PKC-γ (p-PKC-γ) in brain cell fractions obtained from wild-type mice during the LORR induced by these anesthetics. PKC-γ was significantly decreased in the synaptic membrane fraction (P2), whereas p-PKC-γ was significantly increased in P2. There was no significant change in the supernatant fraction (S). In propofol-treated mice, PKC-γ and p-PKC-γ showed no significant changes in P2 or S.
Conclusion : Our results provide new evidence to support the possibility of the involvement of PKC-γ in the actions of volatile anesthetics.
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