The elucidation of the intracerebral transporter control to lead the new cerebroprotection method

• Project/Area Number: 20591818
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Tokyo Medical University
• Principal Investigator: MUROZONO Michihiro Tokyo Medical University, 医学部, 講師 (70276947)
• Co-Investigator(Kenkyū-buntansha): 岡田 真也 東京医科大学, 医学部, 講師 (20297279)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 脳虚血 / mdr1a / 血液脳関門 / P-glycoprotein / サイトカイン / P-glucoprotein / ノックアウトマウス

Research Abstract

There is mdr1a intracranially, which blocks invasion of multi drug to brain. Mdr1 also influences displacement of endogenous substance. We made a focal cerebral ischemia model using mdr1a knockout mouse and measured blood cytokine and apoptosis-related substances such as Bcl-2 in brain. From the results, we suggest that mdr1a influences IL-6, Bcl-2 and Bax after cerebral ischemia and promotes the damage to brain.

Research Products

• [Journal Article] Effects of Differential Types of Anesthetic Agents on Cellular Protein Kinase C-γ Dynamics in Mouse Brain (2011)
  • Author(s): Akiko Takeda, Ryoichi Miyashita, Takeo Nagura, Shusuke Sekine, Michihiro Murozono, et al.
  • Journal Title: Pharmacology 87 Pages: 180-186
  • Peer Reviewed
• [Journal Article] Reduction of Brain Infarction Induced by a Transient brain Ischemia in mdr1a Knockout Mice (2009)
  • Author(s): Michihiro Murozono, Shohei Matsumoto, Sinya Okada, et al.
  • Journal Title: Neurochem Res 34 Pages: 1555-1561
  • Peer Reviewed
• [Journal Article] Reduction of brain infarction induced by a transient brainischemia in mdrla knockout mice (2009)
  • Author(s): Michihiro Murozono
  • Journal Title: Neurochemical research 34 Pages: 1555-1561
  • Peer Reviewed
• [Journal Article] Isoflurane inhibits protein kinase Cgamma and calcium/calmodulin dependent protein kinase ii-alpha translocation to synaptic membranes in ischemic mice brains. (2008)
  • Author(s): Shohei Matsumoto Michihiro Murozono
  • Journal Title: Neurochemical Research 33 Pages: 2302-2309
  • Peer Reviewed
• [Presentation] 二次元レーザー血流計を用いたマウス-過性脳虚血モデルでの脳血流測定 (2010)
  • Author(s): 室園美智博
  • Organizer: 第57回日本麻酔科学会学術集会
  • Place of Presentation: マリンメッセ福岡(福岡)
  • Year and Date: 2010-06-04
• [Presentation] 二次元レーザー血流計を用いたマウス一過性脳虚血モデルでの脳血流測定 (2010)
  • Author(s): 室園美智博, ら
  • Organizer: 日本麻酔科学会第57回学術集会
• [Presentation] Mdrlaノックアウトマウスによる一過性脳虚血でのアポトーシス及びサイトカインに関する検討 (2009)
  • Author(s): 室園美智博
  • Organizer: 日本麻酔科学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-08-16
• [Presentation] mdr1aノックアウトマウスにおける一過性局所脳虚血でのサイトカインに関する検討 (2009)
  • Author(s): 室園美智博, ら
  • Organizer: 第24回日本shock学会総会
• [Presentation] mdr1aノックアウトマウスによる一過性脳虚血でのアポトーシス及びサイトカインに関する検討日本麻酔科学会 (2009)
  • Author(s): 室園美智博, ら
  • Organizer: 第56回学術集会
• [Presentation] 一過性脳虚血におけるアポトーシス及びサイトカインに対する、mdr1a由来p-glycoproteinの影響 (2009)
  • Author(s): 室園美智博, ら
  • Organizer: 日本蘇生学会第28回大会
• [Presentation] Mdrla由来P-glycoproteinの脳虚血への影響(mdrla ノックアウトマウスによる実験より) (2008)
  • Author(s): 室園美智博
  • Organizer: 第23回日本ショック学会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-10
• [Presentation] mdr1a由来P-glycoproteinの脳虚血への影響 (2008)
  • Author(s): 室園美智博, ら
  • Organizer: 第23回日本Shock学会総会
• [Remarks] ホームページ等