Development of a new therapy using i-RNA method in a newly created pulmonary hypertension model in mice
| Project/Area Number |
20591844
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
UEZONO Shoichi Jikei University School of Medicine, 医学部, 教授 (10291676)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 肺高血圧 / マウス / 血管リモデリング / 核内転写因子 / 核酸医薬 / ラット / モノクロタリン / RNA干渉 / 先天性心疾患 / モノグロタリン / KLF5 / 転写因子 |
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| Research Abstract |
Remodeling of pulmonary vasculature (neointimal formation) is a key pathophysiology of development of pulmonary hypertension. The primary purpose of this research is to establish severe form of pulmonary hypertension model in mice. We observed that three-week-long exposure to hypoxic (FiO2=17%) environment with monocrotaline injection caused severe pulmonary hypertension. Unfortunately, more than 50% of these pulmonary hypertensive mice were too sick to tolerate an experiment of measurement of pulmonary artery pressure, necessitating abandon of this experimental model. Using a large cohort of pregnant women with congenital heart disease, we revealed the presence of pulmonary hypertension is a risk factor for maternal and neonatal poor outcome of pregnancy.
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Report
(4 results)
Research Products
(3 results)
