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Plasticity and in Spinal Dorsal Horn Neuron and Neuropathic Pain : An Analysis by Patch Clamp Recordings

Research Project

Project/Area Number 20591845
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKansai College of Oriental Medicine

Principal Investigator

KASHIBA Hitoshi  Kansai College of Oriental Medicine, 保健医療学部, 教授 (10185754)

Co-Investigator(Kenkyū-buntansha) OSHIMA Minoru  関西医療大学, 保健医療学部, 講師 (20342230)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords疼痛管理学 / 神経因性疼痛 / 可塑性 / 脊髄後角ニューロン / 神経科学 / 脳・脊髄 / パッチクランプ法 / 脊髄後角
Research Abstract

The actions of neurotransmitters and modulators have well been investigated in superficial spinal dorsal horn neurons, but little is known about those in deep dorsal horn neurons. Our previous studies suggested that SP, CGRP and somatostatin play important roles in synaptic transmission to deep dorsal horn neurons. In this study, responses of SP and mENK to deep dorsal horn neurons were examined by the blind patch clamp technique used freshly sliced spinal cord of the rat (3-4weeks). About 60% of deep dorsal horn neurons in the deep lamina (III-V) showed the slow inward currents more than 5 pA by the bath application of SP (1 μM) for 1 min. These currents are considered to be evoked though G protein-Coupled SP receptors on the patched cells. Some of the recorded neurons enhanced the number and the peak amplitude of spontaneous EPSCs and/or IPSCs by the application SP. On the other hand, about 60% of the deep dorsal horn neurons showed the slow outward currents more than 5 pA by the bath application of mENK (5 μM), DAMGO (mu-receptor agonist. 1 μM), or DADLE (delta-receptor agonist; 1 μM) for 1 min. These currents are also considered to be evoked though G protein-coupled mu/delta-receptors on the patched cells. The responsive neurons to mENK always displayed the outward current by DAMGO, DADLE, or both. The amplitude of outward current was not suppressed by the repetitive application of mENK. Most of deep dorsal horn neurons with the slow inward currents by SP showed the slow outward currents by enkephalin, DAMGO, or DADLE, and vice versa. The present study suggests that about 60% of deep dorsal horn neurons receive both excitatory inputs though the NK1 receptors and inhibitory inputs though the mu-/delta-receptors.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2011 2010 2008 2007 2004 2003 2002 2001 Other

All Journal Article (7 results) Presentation (3 results) Book (5 results) Remarks (1 results)

  • [Journal Article] TRPV2-immunoreactive intrinsic neurons in the rat intestine.2004

    • Author(s)
      Kashiba H, Uchida Y, Takeda D, Nishigori A, Ueda Y, Kuribayashi K, Minoru Ohshima M.
    • Journal Title

      Neurosci.Lett. 366

      Pages: 193-196

    • Related Report
      2010 Final Research Report
  • [Journal Article] Distribution and colocalization of NGF and GDNF family ligand receptor mRNAs in dorsal root and nodose ganglion neurons of adult rats.2003

    • Author(s)
      Kashiba H, Uchida Y, Senba E
    • Journal Title

      Mol.Brain Res. 110

      Pages: 52-62

    • Related Report
      2010 Final Research Report
  • [Journal Article] Electroacupuncture induces the expression of Fos in rat dorsal horn via capsaicin-insensitive afferents.2003

    • Author(s)
      Uchida Y, Nishigori A, Takeda D, Ohshiro M, Ueda Y, Ohshima M, Kashiba H
    • Journal Title

      Brain Res 978

      Pages: 136-140

    • Related Report
      2010 Final Research Report
  • [Journal Article] NIH「Acupuncture」合意声明に対する日本と米国における受け止め方の違い2003

    • Author(s)
      金田太吾、樫葉均
    • Journal Title

      医道の日本 62巻6号

      Pages: 139-144

    • NAID

      40020372487

    • Related Report
      2010 Final Research Report
  • [Journal Article] 痛覚伝道路の可塑性と神経栄養因子2002

    • Author(s)
      樫葉均, 仙波恵美子
    • Journal Title

      Clinical Neuroscience 20巻

      Pages: 1116-1118

    • Related Report
      2010 Final Research Report
  • [Journal Article] Difference in binding by isolectin B4 to trkA and c-ret mRNA-expressing neurons in rat sensory ganglia.2001

    • Author(s)
      Kashiba H, Uchida Y, Senba E
    • Journal Title

      Mol.Brain Res. 95

      Pages: 18-26

    • Related Report
      2010 Final Research Report
  • [Journal Article] Histamine H1 receptor mRNA is expressed in capsaicin-insensitive sensory neurons with neuropeptide Y-immunoreactivity in guinea pigs.2001

    • Author(s)
      Kashiba H, Fukui H, Senba E
    • Journal Title

      Brain Res. 901

      Pages: 85-93

    • Related Report
      2010 Final Research Report
  • [Presentation] サブスタンスPに応答する脊髄後角の深層ニューロンはエンケファリンにも応答する-パッチクランプ法による解析-2011

    • Author(s)
      樫葉均, 他5名
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      盛岡市
    • Related Report
      2010 Annual Research Report
  • [Presentation] サブスタンスPに応答する脊髄後角の深層ニューロンはエンケファリンにも応答する-パッチクランプ法による解析-2010

    • Author(s)
      清行康邦、中塚映政、武田大輔、内田靖之、大島稔、樫葉均
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      盛岡
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 脊髄後角深層ニューロンの神経ペプチド(SP、CGRP、STT)に対する膜電位応答2008

    • Author(s)
      樫葉均、武田大輔、内田靖之、大島稔
    • Organizer
      第85回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Book] 分担;第3章A 鎮痛(日本伝統医学テキスト鍼灸編)2011

    • Author(s)
      樫葉均
    • Publisher
      医学書院(完成予定)
    • Related Report
      2010 Final Research Report
  • [Book] 日本伝統医学テキスト鍼灸編(第3章A 鎮痛)(2011年11月完成予定)2011

    • Author(s)
      樫葉均(分担)
    • Total Pages
      356
    • Publisher
      医学書院
    • Related Report
      2010 Annual Research Report
  • [Book] Biochemical Basis of Nociception : The Role of Cytokines(The Handbook of Chronic Pain Part I, Chapter 2)2007

    • Author(s)
      Senba E, Imbe H, Kashiba H
    • Publisher
      Nova Science Publishers Inc. New York
    • Related Report
      2010 Final Research Report
  • [Book] 心と体を結ぶ感覚-特に痛みについて-(動きを生み出す心と体のしくみ 第六章)2004

    • Author(s)
      樫葉均(分担)
    • Publisher
      あいり出版
    • Related Report
      2010 Final Research Report
  • [Book] 脳機能の解明-生命科学の主潮流-(慢性痛におけるヒスタミン・セロトニンの関与)2002

    • Author(s)
      仙波恵美子、岡本圭一郎、井辺弘樹、樫葉均
    • Publisher
      ガイア出版
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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