| Project/Area Number |
20591847
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
SHINOHARA Nobuo Hokkaido University, 大学院・医学研究科, 准教授 (90250422)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIDA Kyoko 北海道大学, 大学院・歯学研究科, 准教授 (40399952)
NONOMURA Katsuya 北海道大学, 大学院・医学研究科, 教授 (60113750)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Keywords | 腫瘍血管内皮 / スクリーニング / 薬剤感受性 / 抗がん剤 / 腎癌治療 / 血管新生阻害剤 / 腫瘍血管内皮細胞 / 血管内皮細胞マーカー / 染色体異常 |
|---|
| Research Abstract |
To develope new screening system for anti-angiogenic drugs, we isolated normal endothelial cell (NEC) and tumor endothelial cell (TEC) from human renal cell carcinoma xenografts and from dermis of normal mice, respectively. We analyzed the difference between TEC and NEC and investigate the potential of TEC for the screening system which reflect tumor microenvironment.
Especially, we focused on several genes which were upregulated in TEC compared to NEC by DNA microarray analysis.
We found these genes had strong association with abnormal phenotypes of TECs such as drug resistance, high viability, and migration.
The specific phenotype in mouse TEC were also found in human TEC isolated from human renal cell carcinoma.
These results enable us to approach new anti-angiogenic drug screening system and new anti-angiogenic therapy.
|
