The search of the prostate cancer marker based on blood carbohydrate chain structure and the establishment of the diagnostic method

• Project/Area Number: 20591867
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Juntendo University
• Principal Investigator: FUJIMURA Tsutomu Juntendo University, 医学(系)研究科, 准教授 (70245778)
• Co-Investigator(Kenkyū-buntansha): KAZUNO SAIKO 順天堂大学, 医学(系)研究科(研究院), 助教 (00338344)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 腫瘍学 / 前立腺癌 / 腫瘍マーカー / ハプトグロビン / 糖鎖修飾 / レクチン / PHA-L、SNA、AAL / ELISA / 診断キット / CA19-9 / フコース / シアル酸

Research Abstract

Postate-specific antigen (PSA) has been widely used for detection and diagnosis of prostate cancer. But a false-positive is high in the diagnosis of the prostate cancer when I use PSA. I compared glycosylation haptoglobin as a prostate cancer marker as alternated PSA. The sialylation and fucosylation of the haptoglobin significantly rose with prostate cancer serum .I showed the possibility of a diagnostic method used as tumor marker of the novel prostate cancer that haptoglobin was based on blood carbohydrate structure.

Research Products

• [Journal Article] Liver autophagy contributes to the maintenance of blood glucose and aminoacid levels. (2011)
  • Author(s): Ezaki J., Fujimura T., Ueno T., et al.
  • Journal Title: Autophagy Volume: (in press)
  • Peer Reviewed
• [Journal Article] Discovery of proteinaceous N-modification in lysine biosynthesis of Termus thermophilus. (2009)
  • Author(s): Horie A., Fujimura T., Nishiyama M., et al.
  • Journal Title: Nat Chem Biol 5巻 Pages: 673-679
  • Peer Reviewed
• [Journal Article] Globotriaosylceramide-expressing Burkitt's lymphoma cells are committed to early apoptotic status by rhamnose binding lectin from catfish eggs. (2009)
  • Author(s): Kawano T., Fujimura T., Taka H., Nitta K., et al.
  • Journal Title: Biol Pharm Bull. 32巻 Pages: 345-353
  • NAID: 110007122745
  • Peer Reviewed
• [Journal Article] Mite serine protease activates protease-activated receptor-2 and induces cytokine release in human keratinocytes. (2009)
  • Author(s): Kato T., Takai T., Fujimura T., Ogawa H., et al.
  • Journal Title: Allergy 64巻 Pages: 1366-13674
  • Peer Reviewed
• [Journal Article] Toxic effects of dopamine metabo-lism in Parkinson's disease. (2009)
  • Author(s): Hattori N., Fujimura T., Mochizuki H., et al.
  • Journal Title: Parkinso-nism Relat Disord 15巻 Pages: 35-38
  • Peer Reviewed
• [Journal Article] Toxic effects of dopamine metabolism in Parkinson's disease. (2009)
  • Author(s): Hattoria N., Fujimura T., Mochizuki H., et al.
  • Journal Title: Parkinsonism Relat Disord. Volume: 15 Pages: 35-38
  • Peer Reviewed
• [Journal Article] Globotriaosylceramide-expressing Burkitt's lymphoma cells are committed to early apoptotic status by rhamnose-binding lectin from catfish eggs. (2009)
  • Author(s): Kawano T., Fujimura T., Taka H., Nitta K., et al.
  • Journal Title: Biol Pharm Bull. Volume: 32 Pages: 345-353
  • NAID: 110007122745
  • Peer Reviewed
• [Journal Article] Discovery of proteinaceous N-modification in lysine biosynthesis of Termus thermophilus (2009)
  • Author(s): Horie A., Fujimura T, Nishiyama M., et al.
  • Journal Title: Nat Chem Biol 5 Pages: 673-679
  • Peer Reviewed
• [Journal Article] Mite serine protease activates protease-activated receptor-2 and induces cytokine release in human keratinocytes (2009)
  • Author(s): Kato T., Takai T., Fujimura T., Ogawa H., et al.
  • Journal Title: Allergy 64 Pages: 1366-74
  • Peer Reviewed
• [Journal Article] Haptoglobin-beta chain defined by monoclonal antibody RM2 as a novel serum marker for prostate cancer. (2008)
  • Author(s): Saito S., Fujimura T., Arai Y., et al.
  • Journal Title: Int J Cancer 123巻 Pages: 633-640
  • Peer Reviewed
• [Journal Article] Haptoglobin-beta chain defined by monoclonal antibody RM2 as a novel serum marker for prostate cancer. (2008)
  • Author(s): Saito S., Fujimura T., Arai Y., et al.
  • Journal Title: Int J Cancer 123 Pages: 633-640
  • Peer Reviewed
• [Presentation] 血中のハプトグロビンをバイオマーカーに用いる前立腺ガンの早期診断法 (2010)
  • Author(s): 藤村務、他3名
  • Organizer: BMB2010(日本分子生物・生化学会合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 前立腺癌診断を目的とした血清糖蛋白の糖鎖変化を捉えるSPR多段階解決法の確立 (2010)
  • Author(s): 數野彩子、藤村務、他2名
  • Organizer: BMB2010(日本分子生物・生化学会合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] Identification of nitrotryptophan in the sequence of nitrated proteins generated by the treatment of PC12 celllysate with peroxynitrite. (2009)
  • Author(s): Hiroaki K., Tsutomu F., Fumiyuki Y., 他3名
  • Organizer: The 82^<nd> Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] Identification of nitrotryptophan in the sequence of nitrated proteins generated by the treatment of PC12 cell lysate with peroxynitrite (2009)
  • Author(s): Hiroaki K., Tsutomu F., Fumiyuki Y.
  • Organizer: The 82^<nd> Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] Glycosylation status of haptoglobin in sera of patients with prostate cancer versus benign prostate disease and normal subjects. (2008)
  • Author(s): Fujimura T., Shinohara Y., Hakomori S.
  • Organizer: Biochemistry and Molecular Biology2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] Glycosylation status of haptoglobin in sera of patients with prostate cancer versus benign prostate disease and normal subjects. (2008)
  • Author(s): Tsutomu Fjimura、他4名
  • Organizer: Biochemistry and Molecular Biology 2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-10-23
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] 新規な糖鎖修飾ハプトグロビン (2008)
  • Inventor(s): 五十嵐靖之、藤村務、他3名
  • Industrial Property Rights Holder: 五十嵐靖之、藤村務、他3名
  • Filing Date: 2008-05-29