Analysis of endocrine-related protein involved in prostate cell growth

• Project/Area Number: 20591869
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Nihon University
• Principal Investigator: TAKAHASHI Satoru Nihon University, 医学部, 教授 (50197141)
• Co-Investigator(Kenkyū-buntansha): INOUE Satoshi 東京大学, 医学部・附属病院, 特任教授 (40251251) ; URANO Tomohiko 東京大学, 医学部・附属病院, 特任助教 (20334386) ; HIRANO Daisaku 日本大学, 医学部, 准教授 (40228804)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 腫瘍学 / ゲノム / 前立腺癌 / Androgen / 2011 / 10 / 01 / ACSL3 / ARFGAP3 / 前立腺 / Oct1 / アンドロゲンレセプター / Amyloid Precursor Protein / アンドロゲン / エストロゲン / estrogen-related receptors / estrogen receptor-binding fragment-associated gene 9 / ETS family / Oct-1 / androgen / estrogen / P450 / APP / EBAG9

Research Abstract

(1) We demonstrated the differential expression of estrogen receptor-related protein in prostate cancer. (2) We evaluated CYP3A4 expression in human prostrate cancer tissues. (3) We identified amyloid precursor protein and ARFGAP3 as a primary androgen targetandrogen target genes that are directly regulated by AR in LNCaP cells, by combining chromatin immunoprecipitation (ChIP) with tiling micro-arrays (ChIP-chip). (4) We identified Oct1 correlates with the poorer survival prognosis of the prostate cancer patients.

Research Products

• [Journal Article] Oct1 regulates cell growth of LNCaP cells and is a prognostic factor for prostate cancer. (2011)
  • Author(s): Obinata D, Takayama KI, Urano T, Murata T, Kumagai J, Fujimura T, Ikeda K, Horie-Inoue K, Homma Y, Ouchi Y, Takahashi S, Inoue S
  • Journal Title: International Journal of Cancer (in press)
• [Journal Article] Amyloid precursor protein is a primary androgen target gene that promotes prostate cancer growth. (2011)
  • Author(s): 高山賢一
  • Journal Title: Cancer Research Volume: 69 Pages: 137-42
  • Peer Reviewed
• [Journal Article] Oct1 regulates cell growth of LNCaP cells and is a prognostic factor for prostate cancer. (2011)
  • Author(s): 大日方大亮
  • Journal Title: International Journal of Cancer Volume: (In Press)
  • Peer Reviewed
• [Journal Article] Differential expression of estrogen-related receptors beta and gamma (ERR beta and ERR gamma) and their clinical significance in human prostate cancer. (2010)
  • Author(s): Fujimura T, Takahashi S, Urano T, Ijichi N, Ikeda K, Kumagai J, Murata T, Takayama K, Horie-Inoue K, Ouchi Y, Muramatsu M, Homma Y Inoue S
  • Journal Title: Cancer Science 101 Pages: 646-651
• [Journal Article] Differential expression of estrogen-related receptors β and γ(ERRβ and ERRγ)and their clinical significance in human prostate cancer (2010)
  • Author(s): Fujimura T, Takahashi S, Urano T, Ijichi N, Ikeda K, Kumagai J, Murata T, Takayama K, Horie-Inoue K, Ouchi Y, Muramatsu M, Homma Y, Inoue S
  • Journal Title: Cancer Science 101 Pages: 646-51
  • NAID: 10026589308
  • Peer Reviewed
• [Journal Article] Amyloid precursor protein is a primary androgen target gene that promotes prostate cancer growth. (2009)
  • Author(s): Takayama K, Tsutsumi S, Suzuki T, Horie-Inoue K, Ikeda K, Kaneshiro K, Fujimura T, Kumagai J, Urano T, Sakaki Y, Shirahige K, Sasano H, Takahashi S, Kitamura T, Ouichi Y, Aburatani H, Inoue S
  • Journal Title: Cancer Research 69 Pages: 137-142
• [Journal Article] Expression of cytochrome P450 3A4 and its clinical significance in human prostate cancer. (2009)
  • Author(s): Fujimura T, Takahashi S, Urano T, Kumagai J, Murata T, Takayama K, Ogushi T, Horie-Inoue K, Ouchi Y, Kitamura T, Muramatsu M, Homma Y, Inoue S
  • Journal Title: Urology 74 Pages: 391-397
• [Journal Article] Estrogen receptor-binding fragment-associated gene 9 expression and its clinical significance in human testicular cancer. (2009)
  • Author(s): Fujimura T, Takahashi S, Urano T, Xiaoqiang L, Ogushi T, Muramatsu M, Ouchi Y, Kitamura T, Homma Y, Inoue S
  • Journal Title: Int J Urol 16 Pages: 329-332
  • NAID: 10025197204
• [Journal Article] EBAG9 is a tumor-promoting and prognostic factor for bladder cancer. (2009)
  • Author(s): Kumagai J, Urano T, Ogushi T, Takahashi S, Horie-Inoue K, Fujimura T, Azuma K, Muramatsu M, Ouchi Y, Kitamura T, Inoue S
  • Journal Title: International Journal of Cancer 123 Pages: 799-805
• [Journal Article] Expression of cytochrome P450 3A4 and its clinical significance in human prostate cancer (2009)
  • Author(s): Fujimura T, Takahashi S, Urano T, Kumagai J, Murata T, Ogushi T, Horie-Inoue K, Ouchi Y, Kitamura T, Muramatsu M, Homma Y, Inoue S
  • Journal Title: Urology 74 Pages: 391-397
  • Peer Reviewed
• [Journal Article] Estrogen receptor-binding fragment-associated gene 9 expression and its clinical significance in human testicular cancer (2009)
  • Author(s): Fujimura T, Takahashi S, Urano T, Xiaoqiang L, Ogushi T, Muramatsu M, Ouchi Y, Kitamura T, Homma Y, Inoue S
  • Journal Title: Int J Urol 16 Pages: 329-332
  • NAID: 10025197204
  • Peer Reviewed
• [Journal Article] Amyloid precursor protein is a primary androgen target gene that promotes prostate cancer growth. (2009)
  • Author(s): Takayama K, Tsutsumi S, Suzuki T, Horie-Inoue K, Ikeda K, Kaneshiro K, Fujimura T, Kumagai J, Urano T, Sakaki Y, Shirahige K, Sasano H, Takahashi S, Kitamura T, Ouichi Y, Aburatani H, Inoue S.
  • Journal Title: Cancer Research 69 Pages: 137-142
  • Peer Reviewed
• [Journal Article] EBAG9 is a tumor-promoting and prognostic factor for bladder cancer. (2009)
  • Author(s): Kumagai J, Urano T, Ogushi T, Takahashi S, Horie-Inoue K, Fujimura T, Azuma K, Muramatsu M, Ouchi Y, Kitamura T, Inoue S.
  • Journal Title: International Journal of Cancer 123 Pages: 799-805
  • Peer Reviewed
• [Journal Article] Expression of cytochrome P450 3A4 and its clinical significance in human prostate cancer (2009)
  • Author(s): Fujimura T, Takahashi S, Urano T, Kumagai J, Murata T, Ogushi T, Horie-Inoue K, Ouchi Y, Kitamura T, Muramatsu M, Homma Yukio, Inoue S.
  • Journal Title: Urology (In press)
  • Peer Reviewed
• [Presentation] 前立腺癌においてETS Familyと癒合する遺伝子ACSL3のアンドロゲン応答にOct-1が重要である (2010)
  • Author(s): 大日方大亮
  • Organizer: 第17回日本ステロイドホルモン学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2010-11-14
• [Presentation] 前立腺癌においてETS Familyと癒合する遺伝子ACSL3のアンドロゲン応答にOct-1が重要である (2010)
  • Author(s): 大日方大亮
  • Organizer: 第17回日本ステロイドホルモン学会学術集会
  • Place of Presentation: 福岡県
  • Year and Date: 2010-11-14
• [Presentation] ARFGAP3, an androgen target gene, promotes prostate cancer cell proliferation and migration. (2010)
  • Author(s): 大日方大亮
  • Organizer: The 69th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] 前立腺癌における癒合遺伝子のアンドロゲン応答性に関わるARとOct-1転写因子の協調作用 (2010)
  • Author(s): 大日方大亮, 高山賢一, 浦野友彦, 堀江公仁子, 池田和博, 大内尉義, 高橋悟, 井上聡
  • Organizer: 第19回泌尿器科分子・細胞研究会
  • Place of Presentation: 神戸
  • Year and Date: 2010-02-19
• [Presentation] ARFGAP3 is an androgen-responsive gene that positively promotes proliferation in prostate cancer cell line. (2009)
  • Author(s): 大日方大亮
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10