Study on new treatment strategy for bladder storage dysfunction

• Project/Area Number: 20591875
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Shinshu University
• Principal Investigator: IGAWA Yasuhiko Shinshu University, 医学部附属病院, 特任教授 (40159588)
• Co-Investigator(Kenkyū-buntansha): OGAWA Teruyuki 信州大学, 医学部, 特任助教 (90467142) ; AIZAWA Naoki 東京大学, 医学部附属病院, 特任助教 (80595257)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 排尿学 / 膀胱蓄尿障害 / 求心性神経伝達 / 間質性膀胱炎 / 膀胱再生

Research Abstract

Electrophysiological investigation in the rat revealed that beta3-AR agonists and activation of L-arginine/NO inhibit mechanosensitive afferent nerve activities primarily originating from the bladder in response to bladder distension as well as increased afferent nerve activities induced by intravesical instillation of PGE_2 and acrolein, respectively. Freeze-injured mouse bladders implanted with human amniotic mesenchymal cells recovered morphology and function faster than control-injected bladders. CXCL10 in the bladder urothelium and urine increased in ulcer type interstitial cystitis, suggesting its possible use as a biomarker.

Research Products

• [Journal Article] Effects of nitric oxide on the primary bladder afferent activities of the rat with and without intravesical acrolein treatment. (2011)
  • Author(s): Aizawa N, Igawa Y, Nishizawa O, Wyndaele JJ
  • Journal Title: European Urology 59 Pages: 264-271
  • Peer Reviewed
• [Journal Article] Effects of intravesical instillation of ATP on rat bladder primary afferent activity and its relationship with capsaicin-sensitivity. (2011)
  • Author(s): Aizawa N, Igawa Y, Andersson KE, Iijima K, Nishizawa O, Wyndaele JJ
  • Journal Title: Neurourology and Urodynamics 30 Pages: 163-168
  • Peer Reviewed
• [Journal Article] Relation between expression of α(1)-adrenoceptor mRNAs in bladder mucosa and urodynamic findings in men with lower urinary tract symptoms. (2011)
  • Author(s): Kurizaki Y, Ishizuka O, Imamura T, Ichino M, Ogawa T, Igawa Y, Nishizawa O, Andersson KE
  • Journal Title: Scandinavian Journal of Urology and Nephrology 45 Pages: 15-19
  • Peer Reviewed
• [Journal Article] β-Adrenoceptor agonist effects in experimental models of bladder dysfunction. (2011)
  • Author(s): Michel MC, Ochodnicky P, Homma Y, Igawa Y.
  • Journal Title: Pharmacology & Therapeutics 131 Pages: 40-49
  • Peer Reviewed
• [Journal Article] Effects of nitric oxide on the primary bladder afferent activities of the rat with and without intravesical acrolein treatment. (2011)
  • Author(s): Aizawa N, Igawa Y, Nishizawa O, Wyndaele JJ.
  • Journal Title: European Urology Volume: 59 Pages: 264-271
  • Peer Reviewed
• [Journal Article] Effects of intravesical instillation of ATP on rat bladder primary afferent activity and its relationship with capsaicin-sensitivity. (2011)
  • Author(s): Aizawa N, Igawa Y, Andersson KE, Iijima K, Nishizawa O, Wyndaele JJ.
  • Journal Title: Neurourology and Urodynamics Volume: 30 Pages: 163-168
  • Peer Reviewed
• [Journal Article] Relation between expression of α(1)-adrenoceptor mRNAs in bladder mucosa and urodynamic findings in men with lower urinary tract symptoms. (2011)
  • Author(s): Kurizaki Y, Ishizuka O, Imamura T, Ichino M, Ogawa T, Igawa Y, Nishizawa O, Andersson KE.
  • Journal Title: Scandinavian Journal of Urology and Nephrology Volume: 45 Pages: 15-19
  • Peer Reviewed
• [Journal Article] Comparison of the effects of oestrogen deficiency and old age on primary bladder afferent activity and voiding behaviour in the ageing female rat. (2010)
  • Author(s): Aizawa N, Iijima K, Rosenbaum JS, Downs TR, Igawa Y, Andersson KE, Wyndaele JJ
  • Journal Title: BJU International
  • Peer Reviewed
• [Journal Article] Effects of CL316,243, a beta(3)-adrenoceptor agonist, and intravesical prostaglandin E(2) on the primary bladder afferent activity of the rat. (2010)
  • Author(s): Aizawa N, Igawa Y, Nishizawa O, Wyndaele JJ
  • Journal Title: Neurourology and Urodynamics 29 Pages: 771-776
  • Peer Reviewed
• [Journal Article] Beta3-adrenoceptor agonists : possible role in the treatment of overactive bladder. (2010)
  • Author(s): Igawa Y, Aizawa N, Homma Y
  • Journal Title: Korean Journal of Urology 51 Pages: 811-818
  • Peer Reviewed
• [Journal Article] Differentiation of smooth muscle cells from human amniotic mesenchymal cells implanted in the freeze-injured mouse urinary bladder. (2010)
  • Author(s): Minagawa T, Imamura T, IgawaY, Aizawa N, Ishizuka O, Nishizawa O
  • Journal Title: European Urology 58 Pages: 299-306
  • NAID: 120007107384
  • Peer Reviewed
• [Journal Article] Autologous bone-marrow-derived mesenchymal stem cell transplantation into injured rat urethral sphincter. (2010)
  • Author(s): Kinebuchi Y, Aizawa N, Imamura T, Ishizuka O, Igawa Y, Nishizawa O
  • Journal Title: International Journal of Urology 17 Pages: 359-368
  • NAID: 10027258036
  • Peer Reviewed
• [Journal Article] CXCR3 binding chemokine and TNFSF14 over expression in bladder urothelium of patients with ulcerative interstitial cystitis. (2010)
  • Author(s): Ogawa T, Homma T, Igawa Y, Seki S, Ishizuka O, Imamura T, Akahane S, Homma Y, Nishizawa O
  • Journal Title: The Journal of Urology 183 Pages: 1206-1212
  • Peer Reviewed
• [Journal Article] Beta3-adrenoceptor agonists : possible role in the treatment of overactive Bladder. (2010)
  • Author(s): Igawa Y, Aizawa N, Homma Y.
  • Journal Title: Korean Journal of Urology Volume: 51 Pages: 811-818
  • Peer Reviewed
• [Journal Article] Comparison of the effects of oestrogen deficiency and old age on primary bladder afferent activity and voiding behaviour in the ageing female rat. (2010)
  • Author(s): Aizawa N, Iijima K, Rosenbaum JS, Downs TR, Igawa Y, Andersson KE, Wyndaele JJ.
  • Journal Title: British Journal of Urology International
  • Peer Reviewed
• [Journal Article] Autologous bone-marrow-derived mesenchymal stem cell transplantation into injured rat urethral sphincter. (2010)
  • Author(s): Kinebuchi Y, Aizawa N, Imamura T, Ishizuka O, Igawa Y, Nishizawa O.
  • Journal Title: International Journal of Urology Volume: 17 Pages: 359-368
  • NAID: 10027258036
  • Peer Reviewed
• [Journal Article] CXCR3 binding chemokine and TNFSF14 over expression in bladder urothelium of patients with ulcerative interstitial cystitis. (2010)
  • Author(s): Ogawa T, Homma T, Igawa Y, Seki S, Ishizuka O, Imamura T, Akahane S, Homma Y, Nishizawa O.
  • Journal Title: Journal of Urology Volume: 183 Pages: 1206-1212
  • Peer Reviewed
• [Journal Article] Differentiation of smooth muscle cells from human amniotic mesenchymal cells implanted in the freeze-injured mouse urinary bladder. (2010)
  • Author(s): Minagawa T, Imamura T, Igawa Y, Aizawa N, Ishizuka O, Nishizawa O.
  • Journal Title: European Urology Volume: 58 Pages: 299-306
  • NAID: 120007107384
  • Peer Reviewed
• [Journal Article] Effects of CL316, 243, a beta 3-adrenoceptor agonist, and intravesical prostaglandin E2 on the primary bladder afferent activity of the rat. (2010)
  • Author(s): Aizawa N, Igawa Y, Nishizawa O, Wyndaele JJ.
  • Journal Title: Neurourology and Urodynamics Volume: 29 Pages: 771-776
  • Peer Reviewed
• [Journal Article] Differentiation of Smooth Muscle Cells from Human Amniotic Mesenchymal cell implantation in the Freeze-Injured Mouse Urinary Bladder (2010)
  • Author(s): Minagawa T, Imamura T, Igawa Y, Aizawa N, Ishizuka O, Nishizawa O
  • Journal Title: European Urology (E-published)
  • NAID: 120007107384
  • Peer Reviewed
• [Journal Article] Mechanosensitive primary bladder afferent activity in rats with and without spinal cord transaction. (2009)
  • Author(s): Iijima K, Igawa Y, Wyndaele JJ, De Wachter S
  • Journal Title: The Journal of Urology 182 Pages: 1504-1510
  • Peer Reviewed
• [Journal Article] Mechanosensitive primary bladder afferent activity in rats with and without spinal cord transection (2009)
  • Author(s): Iijima K, Igawa Y, Wyndaele JJ, De Wachter S
  • Journal Title: The Journal of Urology 182 Pages: 2504-2510
  • Peer Reviewed
• [Journal Article] Effects of CL316, 243, a beta (3)-adrenoceptor agonist, and intravesical prostaglandin E (2) on the primary bladder afferent activity of the rat (2009)
  • Author(s): Aizawa N, Igawa Y, Nishizawa O, Wyndaele JJ
  • Journal Title: Neurourology and Urodynamics (E-published)
  • Peer Reviewed
• [Presentation] Lower urinary tract reconstruction using tissue engineering. (2010)
  • Author(s): 井川靖彦
  • Organizer: Taiwanees Continence Society Annual Meeting.
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-10-17
• [Presentation] Lower urinary tract reconstruction using tissue engineering. (2010)
  • Author(s): Igawa Y.
  • Organizer: Taiwanees Continence Society Annual Meeting
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-10-17
• [Presentation] The beta3-adrenoceptor agonist : Its possible roles in OAB treatment. (2010)
  • Author(s): 井川靖彦
  • Organizer: OAB symposium Taipei.
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-10-16
• [Presentation] The beta3-adrenoceptor agonist : Its possible roles in OAB treatment. (2010)
  • Author(s): Igawa Y.
  • Organizer: OAB symposium Taipei
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-10-16
• [Presentation] 膀胱知覚受容のメカニズム (2010)
  • Author(s): 井川靖彦
  • Organizer: 京都府立医科大学大学院・特別講義
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-05
• [Presentation] In vivo単一神経活動導出法による膀胱求心性神経の機能解析 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第17回日本排尿機能学会(ワークショップ1求心性神経伝達と下部尿路機能)
  • Place of Presentation: 甲府市
  • Year and Date: 2010-09-30
• [Presentation] ベータ3アドレナリン受容体作動薬 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第17回日本排尿機能学会(シンポジウム1.下部尿路機能障害の薬物療法-現状と展望)
  • Place of Presentation: 甲府市
  • Year and Date: 2010-09-30
• [Presentation] ベータ3アドレナリン受容体作動薬 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第17回日本排尿機能学会シンポジウム1下部尿路機能障害の薬物療法-現状と展望
  • Place of Presentation: 甲府市
  • Year and Date: 2010-09-30
• [Presentation] In vivo単一神経活動導出法による膀胱求心性神経の機能解析 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第17回日本排尿機能学会ワークショップ1求心性神経伝達と下部尿路機能
  • Place of Presentation: 甲府市
  • Year and Date: 2010-09-30
• [Presentation] メタボリックシンドロームと下部尿路症状 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第75回日本泌尿器科学会東部総会(シンポジウム2. メタボリックシンドロームと泌尿器疾患)
  • Place of Presentation: 宇都宮市
  • Year and Date: 2010-09-15
• [Presentation] メタボリックシンドロームと下部尿路症状 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第75回日本泌尿器科学会東部総会 シンポジウム2メタボリックシンドロームと泌尿器疾患
  • Place of Presentation: 宇都宮市
  • Year and Date: 2010-09-15
• [Presentation] A new urethral lengthening procedure using intestinal tube for sphincteric incontinence in patients with myelodysplasia. (2010)
  • Author(s): Igawa Y, Kato H, Ichino M, Nishizawa O
  • Organizer: 23rd International Symposium on Paediatric Surgical Research.
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-14
• [Presentation] Urodynamic risk factors against spontaneous resolution of primary vesico-ureteral reflux in male infants. (2010)
  • Author(s): Igawa Y, Ichino M, Nishizawa O
  • Organizer: 23rd International Symposium on Paediatric Surgical Research.
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-14
• [Presentation] Urodynamic risk factors against spontaneous resolution of primary vesico-ureteral reflux in male infants. (2010)
  • Author(s): Igawa Y, Ichino M, Nishizawa O.
  • Organizer: 23rd International Symposium on Paediatric Surgical Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-14
• [Presentation] A new urethral lengthening procedure using intestinal tube for sphincteric incontinence in patients with myelodysplasia. (2010)
  • Author(s): Igawa Y, Kato H, Ichino M, Nishizawa O.
  • Organizer: 23rd International Symposium on Paediatric Surgical Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-14
• [Presentation] 小児下部尿路機能障害の診断と治療 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第9回小児泌尿器教育セミナー(第19回日本小児泌尿器科学会)
  • Place of Presentation: 札幌市
  • Year and Date: 2010-07-01
• [Presentation] 神経因性膀胱の診断と治療の進歩 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第47回日本リハビリテーション医学会学術集会(シンポジウム7排尿障害のリハビリテーションの進歩)
  • Place of Presentation: 鹿児島市
  • Year and Date: 2010-05-22
• [Presentation] 神経因性膀胱の診断と治療の進歩 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第47回日本リハビリテーション医学会学術集会シンポジウム7排尿障害のリハビリテーションの進歩
  • Place of Presentation: 鹿児島市
  • Year and Date: 2010-05-22
• [Presentation] 神経因性膀胱の治療 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-04-29
• [Presentation] 脊髄脂肪腫に伴う下部尿路機能障害の特徴および脂肪腫摘出術前後での変化 (2010)
  • Author(s): 井川靖彦, 市野みどり, 小川輝之, 石塚修, 西沢理
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-04-27
• [Presentation] Characteristics of neurogenic lower urinary tract dysfunction associated with spinal lipoma and : impact of lipoma removal. (2010)
  • Author(s): Igawa Y, Ichino M, Ogawa T, Nishizawa O
  • Organizer: 25th Annual European Association of Urology Congress.
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-04-19
• [Presentation] Characteristics of neurogenic lower urinary tract dysfunction associated with spinal lipoma and : impact of lipoma removal. (2010)
  • Author(s): Igawa Y, Ichino M, Ogawa T, Nishizawa O.
  • Organizer: 25th Annual European Association of Urology Congress
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-04-19
• [Presentation] Expression level of CXCL10 peptide in bladder urothelium and urine aspossible biomarkers for diagnosis of ulcerative interstitial cystitis. (2010)
  • Author(s): Imamura T, Igawa Y, Ogawa T, Homma T, Seki S, Ishizuka O, Akahane S, Homma Y, Nishizawa O
  • Organizer: 25th Annual European Association of Urology Congress.
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-04-18
• [Presentation] Expression level of CXCL10 peptide in bladder urothelium and urine as possible biomarkers for diagnosis of ulcerative interstitial cystitis. (2010)
  • Author(s): Imamura T, Igawa Y, Ogawa T, Homma T, Seki S, Ishizuka O, Akahane S, Homma Y, Nishizawa O.
  • Organizer: 25th Annual European Association of Urology Congress
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-04-18
• [Presentation] 尿路再建術の現状と今後の展望 (2010)
  • Author(s): 井川靖彦
  • Organizer: 第1回山梨排尿障害セミナー
  • Place of Presentation: 甲府市
  • Year and Date: 2010-04-08
• [Presentation] 過活動膀胱の病態生理UPDATE (2010)
  • Author(s): 井川靖彦
  • Organizer: 京都排尿障害フォーラム
  • Place of Presentation: 京都市
  • Year and Date: 2010-03-19
• [Presentation] Lower urinary tract dysfunction after radical hysterectomy. (2010)
  • Author(s): Ichino M, Igawa Y, Ishizuka O, Nishizawa O
  • Organizer: International continence Society-International Urogynecology Association joint Annual Meeting.
  • Place of Presentation: Toronto, Canada
• [Presentation] Relation between expression of ・_1-adrenoceptor mRNAs in bladder mucosa and urodynamic findings in men with lower urinary tract symptoms. (2010)
  • Author(s): Kurizaki Y, Ishizuka O, Imamura T, Ichino M, Ogawa T, Igawa Y, Nishizawa O, Andersson K
  • Organizer: International continence Society-International Urogynecology Association joint Annual Meeting.
  • Place of Presentation: Toronto, Canada
• [Presentation] Saline loading caused the change of the expression of A1-adrenoceptor subtypes and angiotensin II type1 receptors in the prostate of spontaneously hypertensive rats. (2010)
  • Author(s): Ishizuka O, Nakayama T, Imamura T, Kurizaki Y, Igawa Y, Nishizawa O, Andersson K
  • Organizer: International continence Society-International Urogynecology Association joint Annual Meeting.
  • Place of Presentation: Toronto, Canada
• [Presentation] Effects of L-arginine, an no donor, on the primary afferent activity withor without acrolein-Treated rat bladder. International continence Society- (2010)
  • Author(s): Aizawa N, Igawa Y, Wyndaele J
  • Organizer: International Urogynecology Association joint Annual Meeting.
  • Place of Presentation: Toronto, Canada
• [Presentation] Effects of L-arginine, an no donor, on the primary afferent activity with or without acrolein-Treated rat bladder. (2010)
  • Author(s): Aizawa N, Igawa Y, Wyndaele J.
  • Organizer: International continence Society-International Urogynecology Association joint Annual Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] Saline loading caused the change of the expression of A1-adrenoceptor subtypes and angiotensin II typel receptors in the prostate of spontaneously hypertensive rats. (2010)
  • Author(s): Ishizuka O, Nakayama T, Imamura T, Kurizaki Y, Igawa Y, Nishizawa O, Andersson K.
  • Organizer: International continence Society-International Urogynecology Association joint Annual Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] Relation between expression of A1-adrenoceptor mRNAs in bladder mucosa and urodynamic findings in men with lower urinary tract symptoms. (2010)
  • Author(s): Kurizaki Y, Ishizuka O, Imamura T, Ichino M, Ogawa T, Igawa Y, Nishizawa O, Andersson K.
  • Organizer: International continence Society-International Urogynecology Association joint Annual Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] Lower urinary tract dysfunction after radical hysterectomy. (2010)
  • Author(s): Ichino M, Igawa Y, Ishizuka O, Nishizawa O.
  • Organizer: International continence Society-International Urogynecology Association joint Annual Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] Effects of CL316, 243, a beta3-adrenoceptor agonist, and prostaglandin E2 on the primary bladder afferent activity of the rat. (2009)
  • Author(s): Igawa Y, Aizawa N, Wyndaele JJ
  • Organizer: 39^<th> Annual Meeting of International Continence Society
  • Place of Presentation: Sun Francisco, USA
  • Year and Date: 2009-10-03
• [Presentation] Effects of CL316, 246, a beta3-adrenoceptor agonist, and prostaglandin E2 on the primary bladder afferent activity of the rat (2009)
  • Author(s): Igawa Y, Aizawa N, Wyndaele JJ
  • Organizer: 39^<th> Annual Meeting of International Continence Society
  • Place of Presentation: Sun Francisco, USA
  • Year and Date: 2009-10-03
• [Presentation] Effects of intravesical capsaicin, resiniferatoxin and ATP on primary bladder afferent activity of the rat. (2008)
  • Author(s): Igawa Y, Aizawa N, Iijima K, Wyndaele JJ, Andersson K-E, Nishizawa O
  • Organizer: 38^<th> Annual Meeting of International Continence Society.
  • Place of Presentation: Cairo, Egypt
  • Year and Date: 2008-10-24
• [Presentation] Effects of intravesical capsajcin, resiniferatoxin and ATP on primary bladderafferent activity of the rat (2008)
  • Author(s): Igawa Y, Aizawa N, lijima K, Wyndaele JJ, Andersson K-E, Nishizawa o
  • Organizer: 38^<th> Annual Meeting of International Continence Society
  • Place of Presentation: Cairo, Egypt
  • Year and Date: 2008-10-24
• [Remarks] ホームページ等
• [Remarks] コンチネンス医学講座
  • URL: http://cont-med.umin.jp/