| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
We examined the involvement of signal transducer and activator of transcription3(STAT3) in bladder outlet obstruction (BOO)-induced bladder smooth muscle hypertrophy using rat in vivo and in vitro study. BOO induced an increase in bladder weight and the thickness of bladder smooth muscle layer 1 week after operation. In the profiling study, we found an increase in the expression of STAT3. Significant increase in the nuclear phosphorylated STAT3 expression was confirmed in bladder smooth muscle tissue by immunohistochemistry and Western blot analysis. Cyclical stretch-relaxation (1 Hz) at 120% elongation significantly increased the expression of STAT3 and α-smooth muscle actin in primary cultured bladder smooth muscle cells. Furthermore, blockade of STAT3 expression by the transfection of STAT3 small interfering RNA (siRNA) significantly prevented stretch-induced increase in α-smooth muscle actin expression. These results suggest that STAT3 has an important role in the induction of bladder smooth muscle hypertrophy.
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