Analysis of genome maintenance of testicular stem cells in DNA deficient mice with impaired spermatogenesis.

• Project/Area Number: 20591898
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Tottori University
• Principal Investigator: NAKANE Hironobu Tottori University, 医学部, 助教 (10304205)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: DNA修復欠損マウス / 精子形成 / 精巣幹細胞

Research Abstract

We have showed that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] provide a valuable model for growth retardation, internal tumor formation, impaired spermatogenesis as well as for UV-induced skin tumors in XPA patients. Gene expression analysis of each aged Xpa mice testis showed a different expression pattern of DNA repair gene, cell cycle-related gene(checkpoint), apoptosis-related gene compared to control. We found other pathological changes of the aged Xpa mice testis. These data will help us to understand the impaired spermatogenesis in the Xpa mice.

Research Products

• [Journal Article] Histopathological analysis of impaired spermatogenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice (2011)
  • Author(s): Hironobu Nakane, Kiyoji Tanaka, Toshio Kameie, Sumire Inaga, Tomonori Naguro
  • Journal Title: The Journal of Physiological Sciences Vol.61.supple.1
• [Journal Article] Differential DNA damage signaling accounts for distinct neural apoptotic responses in DNA repair deficient mice of ATLD, NBS, XPA, XPG-Insight from neuropathology of DNA repair-deficiency disorders- (2011)
  • Author(s): Hironobu Nakane
  • Journal Title: 1^<st> RIRBM International Symposium-Genome Damage and Non-Cancerous Diseases-抄録集 Pages: 16-18
• [Journal Article] Histopathological analysis of impaired spermatogenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice (2011)
  • Author(s): Hironobu Nakane, et al.
  • Journal Title: The Journal of Physiological Sciences Volume: 61.supple.1
• [Journal Article] コケイン症候群のモデル動物としてのXpgヌルマウスの検討 (2010)
  • Author(s): 中根裕信, 他
  • Journal Title: 第4回日本エピジェネティクス研究会年会要旨集 Pages: 94-94
• [Journal Article] DNA修復異常症におけるDNA損傷応答の違いによる脳神経病委への関与-ATLD,NBS,XPAの神経病変 (2010)
  • Author(s): 中根裕信, 他
  • Journal Title: BMB2010 categ2.workshop.pdf抄録集 Pages: 134-134
• [Journal Article] ヌクレオチド除去修復欠損マウス-転写異常とその病態- (2009)
  • Author(s): 中根裕信
  • Journal Title: 医学のあゆみ 228巻 Pages: 157-158
• [Journal Article] XP・CSモデルマウスの分子病態 (2009)
  • Author(s): 中根裕信
  • Journal Title: 色素性乾皮症全国大会(難病指定記念講演会)報告集 Pages: 5-5
• [Journal Article] ヌクレオチド除去修復欠損マウス 転写異常とその病態(解説/特集) (2009)
  • Author(s): 中根裕信
  • Journal Title: 医学のあゆみ 1月10日号 228 Pages: 157-158
• [Journal Article] Impaired spermatogenesis and elevated spontaneous tumorigenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice. (2008)
  • Author(s): Hironobu Nakane, Seiichi Hirota, Philip J.Brooks, Yusaku Nakabeppu, Yoshimichi Nakatsu, Yoshitake Nishimune, Akihiro Iino, Kiyoji Tanaka
  • Journal Title: DNA Repair (Amst). vol.7(No.12) Pages: 1938-1950
  • Peer Reviewed
• [Journal Article] Impaired spermatogenesis and elevated spontaneous tumorigenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice (2008)
  • Author(s): Nakane H., et al.
  • Journal Title: DNA Repair 7 Pages: 1938-1950
  • Peer Reviewed
• [Presentation] Differential DNA damage signaling accounts for distinct neural apoptotic responses in DNA repair deficient mice of ATLD, NBS, XPA, XPG -Insight from neuropathology of DNA repair-deficiency disorders- (2011)
  • Author(s): 中根裕信
  • Organizer: 1^<st> RIRBM Intemational Symposium -Genome Damage and Non-Cancerous Diseases- (invited speaker)
  • Place of Presentation: Koujin Conference Hall on Kasumi Campus, Hiroshima University
• [Presentation] Differential DNA damage signaling accounts for distinct neural apoptotic responses in DNA repair deficient mice of ATLD, NBS, XPA, XPG-Insight from neuropathology of DNA repair-deficiency disorders- (2011)
  • Author(s): Hironobu Nakane
  • Organizer: 1^<st> RIRBM International Symposium-Genome Damage and Non-Cancerous Diseases-
  • Place of Presentation: Koujin Conference Hall on Kasumi Campus, Hiroshima University
• [Presentation] DNA修復異常症におけるDNA損傷応答の違いによる脳神経病変への関与-ATLD, NBS, XPAの神経病変 (2010)
  • Author(s): 中根裕信、Erin R.P.Shull、Youngsoo Lee、栗政明弘、稲賀すみれ、名黒知徳、田中亀代次、Peter J.McKinnon
  • Organizer: BMB2010 workshop (招待講演)
  • Place of Presentation: 神戸国際展示場(兵庫県神戸市)
  • Year and Date: 2010-12-08
• [Presentation] DNA修復異常症におけるDNA損傷応答の違いによる脳神経病変への関与-ATLD,NBS,XPAの神経病変 (2010)
  • Author(s): 中根裕信, 他
  • Organizer: BMB2010
  • Place of Presentation: 神戸国際展示場(兵庫県神戸市)
  • Year and Date: 2010-12-08
• [Presentation] 色素性乾皮症G群遺伝子欠損マウス(XPG nullマウス)の病理学的解析 (2010)
  • Author(s): 中根裕信、広田誠一、塩見忠博、大浜栄作、亀家俊夫、稲賀すみれ、名黒知徳
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手県民会館(盛岡市)
  • Year and Date: 2010-03-30
• [Presentation] 色素性乾皮症G群遺伝子欠損マウス(XPG null マウス)の病理学的解析 (2010)
  • Author(s): 中根裕信
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手県民会館(盛岡市)
  • Year and Date: 2010-03-30
• [Presentation] CSモデルマウスの解析 (2010)
  • Author(s): 中根裕信
  • Organizer: コケイン症候群(CS)の集い2010
  • Place of Presentation: ロレアル東京アカデミー(東京都渋谷区代々木)
• [Presentation] コケイン症候群のモデル動物としてのXpgヌルマウスの検討 (2010)
  • Author(s): 中根裕信、広田誠一、塩見忠博、大浜栄作、亀家俊夫、稲賀すみれ、名黒知徳
  • Organizer: 第4回日本エピジェネティクス研究会
  • Place of Presentation: 米子市文化ホール(米子市)
• [Presentation] コケイン症候群のモデル動物としてのXpgヌルマウスの検討 (2010)
  • Author(s): 中根裕信, 他
  • Organizer: 第4回日本エピジェネティクス研究会
  • Place of Presentation: 米子市文化ホール(鳥取県米子市)
• [Presentation] CSモデルマウスの解析 (2010)
  • Author(s): 中根裕信
  • Organizer: コケイン症候群(CS)の集い2010
  • Place of Presentation: ロレアル 東京アカデミー(東京都渋谷区代々木)
• [Presentation] Impaired spermatogenesis and elevated spontaneous tumorigenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice (2009)
  • Author(s): 中根裕信, 広田誠一, 田中亀代次
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山理科大学(岡山県)
  • Year and Date: 2009-03-29
• [Presentation] Impaired spermatogenesis and elevated spontaneous tumorigenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice (2009)
  • Author(s): Nakane H., et al.
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山県、岡山理科大学
  • Year and Date: 2009-03-29
• [Presentation] XP・CS モデルマウスの分子病態 (2008)
  • Author(s): 中根裕信
  • Organizer: 色素性乾皮症全国大会(難病指定記念講演会)
  • Place of Presentation: 愛知県、あいち健康プラザ
  • Year and Date: 2008-11-01
• [Presentation] Pathological analysis of XPG-deficient mice (2008)
  • Author(s): Nakane H., et al.
  • Organizer: The 6th 3R Symposium(3R Symposium 2008)
  • Place of Presentation: Kakegawa, Shizuoka
  • Year and Date: 2008-10-27
• [Presentation] Hypersensitivity to oxidative damage in the developing nervous system of XPA mice (2008)
  • Author(s): Nakane H., et al.
  • Organizer: The International Ataxia-Telangiectasia Workshop 2008
  • Place of Presentation: Ohtsu, Shiga
  • Year and Date: 2008-04-22
• [Presentation] XP・CSモデルマウスの分子病態 (2008)
  • Author(s): 中根裕信
  • Organizer: 色素性乾皮症全国大会(難病指定記念講演会)
  • Place of Presentation: あいち健康プラザ(愛知県)
• [Presentation] Pathological analysis of XPG-deficient mice (2008)
  • Author(s): 中根裕信, 広田誠一, 塩見忠博, 大浜栄作, 亀家俊夫, 稲賀すみれ, 名黒知徳
  • Organizer: The 6^<th> 3R Symposium (3R Symposium 2008)
  • Place of Presentation: Tsumagoi Resort (Kakegawa, Shizuoka)
• [Presentation] Hypersensitivity to oxidative damage in the developing nervous system of XPA mice (2008)
  • Author(s): 中根裕信, 栗政明弘, 田中亀代次
  • Organizer: The International Ataxia-Telangiectasia Workshop 2008
  • Place of Presentation: Ohtsu Prince Hotel (Ohtsu, Shiga)