| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Xenotransplantation could provide a solution to the critical shortage of organs for transplantation in humans. In attempt to prevent hyperacute rejection (HAR) and acute humoral xenograft rejection (AHXR), α1,3-galactosyltransferase gene knockout (GalT-KO) pigs have been produced in Massachusetts General Hospital, Boston, USA. In heart xenotransplantation from GalT-KO pig into baboon, heterotopic heart grafts could beat in abdominal cavity by 179 days (median 78 days). In kidney xenotransplantation, GalT-KO kidney grafts rejected by day 34 in chronic immunosuppression protocol, but grafts could survive up to 83 days in tolerance inducing protocol. GalT-KO heart and kidney xenografts did not develop hyperacute rejection. However, all GalT-KO heart and kidney grafts in the chronic immunosuppression group were rejected by AHXR with thrombotic microangiopathy. Acute cellular xenograft rejection (ACXR) and chronic rejection also developed. However, in the kidney grafts in tolerance inducing protocol, AHRX, ACXR, and chronic rejection could not be detected in the grafts. Therefore, we conclude that the tolerance inducing protocol is necessary for success the heart and kidney xenotransplantation from GalT-KO pig into baboons.
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