Analysis of the sperm maturation mechanism by androgen signaling
Project/Area Number |
20591903
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Obstetrics and gynecology
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Research Institution | Tohoku University |
Principal Investigator |
SUZUKI Kichiya Tohoku University, 研究所, 主任研究員 (30422116)
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Co-Investigator(Kenkyū-buntansha) |
寺田 幸弘 東北大学, 病院, 准教授 (10260431)
高橋 藍子 東北大学, 病院, 技能補佐員 (30436125)
長谷川 久隆 東北大学, 病院, 技能補佐員 (20455835)
村上 節 東北大学, 大学院・医学系研究科, 准教授 (20240666)
|
Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 精巣上体 / 精子成熟 / アンドロゲンレセプター / コンディショナルノックアウトマウス / コンデイショナルノックアウトマウス |
Research Abstract |
In order to examine androgen-signaling pathway in the epididymis, we have attempted to establish a conditional knock out mice of the androgen receptor gene in the initial segment of the epididymis. We first generated a transgenic mouse carrying a tamoxifen-regulated Cre recombinase gene driven by the epididymis-specific lipocalin gene promoter. When the mice was crossbred with a ROSA26-EGFP mice to confirm the Cre expression in the epididymis, unexpectedly the EGFP expression was recognized in the cauda epididymis. Although the Cre mice was crossbred with a mice carrying a conditional androgen receptor allele to generate a conditional androgen receptor knock out mice in the epididymis, the mice demonstrated a normal sperm concentration and mobility.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] The Role of Foxa Proteins in the Regulation of Androgen Receptor Activity.2009
Author(s)
De Graff DJ, Yu X, Sun Q, Mirosevich J, Jin RJ, Wang Y, Gupta A, Nandana S, Case T, Paul M, Huang HY, Shapiro E, Logan S, Suzuki K, Orgebin-Crist MC, Matusik RJ
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Journal Title
Androgen Action in Prostate Cancer(Tindall D.and Mohler J.eds)(Springer Science)
Pages: 587-615
Related Report
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[Journal Article]2009
Author(s)
De Graff DJ
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Journal Title
Androgen Action in Prostate Cancer(Springer Science)
Pages: 587-615
Related Report
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[Journal Article] Intramanchette transport during primate spermiogenesis : expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis.2008
Author(s)
Hayasaka S, Terada Y, Suzuki K, Murakawa H, Tachibana I, Sankai T, Murakami T, Yaegashi N, Okamura K
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Journal Title
Asian J Androl 10
Pages: 561-8
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