| Project/Area Number |
20591903
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
SUZUKI Kichiya Tohoku University, 研究所, 主任研究員 (30422116)
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| Co-Investigator(Kenkyū-buntansha) |
寺田 幸弘 東北大学, 病院, 准教授 (10260431)
高橋 藍子 東北大学, 病院, 技能補佐員 (30436125)
長谷川 久隆 東北大学, 病院, 技能補佐員 (20455835)
村上 節 東北大学, 大学院・医学系研究科, 准教授 (20240666)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 精巣上体 / 精子成熟 / アンドロゲンレセプター / コンディショナルノックアウトマウス / コンデイショナルノックアウトマウス |
|---|
| Research Abstract |
In order to examine androgen-signaling pathway in the epididymis, we have attempted to establish a conditional knock out mice of the androgen receptor gene in the initial segment of the epididymis. We first generated a transgenic mouse carrying a tamoxifen-regulated Cre recombinase gene driven by the epididymis-specific lipocalin gene promoter. When the mice was crossbred with a ROSA26-EGFP mice to confirm the Cre expression in the epididymis, unexpectedly the EGFP expression was recognized in the cauda epididymis. Although the Cre mice was crossbred with a mice carrying a conditional androgen receptor allele to generate a conditional androgen receptor knock out mice in the epididymis, the mice demonstrated a normal sperm concentration and mobility.
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