The new treatment strategy for the perinatal brain damage with the mesenchymal stem cell derived from fetal appendages

• Project/Area Number: 20591914
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: KANAGAWA Takeshi Osaka University, 大学院・医学系研究科, 助教 (40346218)
• Co-Investigator(Kenkyū-buntansha): TOMIMATSU Takuji 大阪大学, 大学院・医学系研究科, 助教 (30346209) ; TANIGUCHI Yukiko 大阪大学, 大学院・医学系研究科, 助教 (60423175)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 新生児低酸素虚血性脳障害 / 胎児付属物 / 幹細胞 / CRP / β-ラクタム抗生剤 / プレコンディショニング / 臍帯血管内皮細胞 / 新生児脳障害 / 可溶性fms様チロシンキナーゼ(sFltl) / 可溶性エンドグリン(sEng) / NF-κB / STIC法

Research Abstract

The Perinatal brain damage due to the fetal distress or neonatal distress remains the most important problem although the perinatal care develops rapidly remarkably.
The aims of this research are the decrease the perinatal damage. In this research, we discovered that C-Reactive protein makes the immature brain susceptible to Hypoxic ischemic insult, even if the insult causes little or no injury by itself. We also clarified that the preconditioning by the beta-lactam antibiosis protects the immature brain damage.

Research Products

• [Journal Article] Impaired delivery outcomes in pregnancies following myomectomy compared to myoma-complicated pregnancies. (2011)
  • Author(s): Kinugasa-Taniguchi Y, Ueda Y, Hara-Oyagi C, Enomoto T, Kanagawa T, Kimura T
  • Journal Title: J Reprod Med Journal Reproductive Medicine 3-4 Pages: 142-148
  • Peer Reviewed
• [Journal Article] Ceftriaxone preconditioning confers neuroprotection in neonatal rats through glutamate transporter 1 upregulation. (2010)
  • Author(s): Mimura K, Tomimatsu T, Minato K, Otgonbaatar Jugder, Kinugasa-Taniguchi Y, Kanagawa T, Nozaki M, Yanagihara I, Kimura T.
  • Journal Title: Reprod Sci in press
  • Peer Reviewed
• [Journal Article] A retrospective analysis of ovarian endometriosis during pregnancy. (2010)
  • Author(s): Ueda Y, Enomoto T, Miyatake T, Fujita M, Yamamoto R, Kanagawa T, Shimizu H, Kimura T
  • Journal Title: Fertil Steril 94 Pages: 78-84
  • Peer Reviewed
• [Journal Article] Current status of negative treatment decision-making for fetuses with a prenatal diagnosis of neonatal surgical disease at a single Japanese institution. (2010)
  • Author(s): 臼井規朗, 金川武司, 上山雅史, 谷人, 谷口(衣笠)友基子, 木村正, 福澤正洋
  • Journal Title: J Pediatr Surg 45 Pages: 2328-33
  • Peer Reviewed
• [Journal Article] Maternal blood serum and plasma human tumor- associated antigen RCAS1 during the course of uncomplicated pregnancies : a prospective study. (2010)
  • Author(s): Tskitishvili E, Sharentuya N, Tsubouchi H, Kinugasa-Taniguchi Y, Kanagawa T, Shimoya K, Tomimatsu T, Kimura T
  • Journal Title: Am J Reprod Immunol 64 Pages: 218-24
  • Peer Reviewed
• [Journal Article] Nicotine suppresses interleukin-6 production from vascular endothelial cells : a possible therapeutic role of nicotine for preeclampsia. (2010)
  • Author(s): Sharentuya N, Tomimatsu T, Mimura K, Tskitishvili E, Kinugasa-Taniguchi Y, Kanagawa T, Kimura T
  • Journal Title: Reprod Sci 17 Pages: 556-63
  • Peer Reviewed
• [Journal Article] Nicotine restores endothelial dysfunction caused by excess sFlt1 and sEng in in vitro model of preeclamptic vascular endothelium : apossible therapeutic role of nicotinic acetylcholine receptor (nAChR) agoonists for preeclampsia. (2010)
  • Author(s): Mimura K, Tomimatsu T, Sharentuya N, Tskitishvili E, Kimura T, Kimura T.
  • Journal Title: Am J Obstet Gynecol 202
  • Peer Reviewed
• [Journal Article] Human C-reactive protein enhances vulnerability of immature ratstohypoxic-ischemic brain damage ; a preliminary study. (2010)
  • Author(s): Kinugasa-Taniguchi Y, Tomimatsu T, Mimura K, Kanagawa T, Shimoya K, Murata Y, Kimura T
  • Journal Title: Reprod Sci 17 Pages: 419-25
  • Peer Reviewed
• [Journal Article] 出生前に消極的治療方針が選択された新生児外科症例の検討. (2010)
  • Author(s): 臼井規朗, 金川武司, 上山雅史, 谷人, 谷口(衣笠)友基子, 木村正, 福澤正洋
  • Journal Title: 日本周産期・新生児医学会誌 46 Pages: 32-8
  • NAID: 10026414098
  • Peer Reviewed
• [Journal Article] 出生前診断された腹壁異常症例における両親の治療方針選択と予後に関する検討 (2010)
  • Author(s): 臼井規朗, 金川武司, 上山雅史, 谷人, 福澤正洋
  • Journal Title: 日本小児外科学会誌 46 Pages: 229-34
  • Peer Reviewed
• [Journal Article] Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells. (2010)
  • Author(s): Tskitishvili E, Sharentuya N, Temma-Asano K, Mimura K, Kinugasa-Taniguchi Y, Kanagawa T, Fukuda H, Kimura T, Tomimatsu T, Shimoya K.
  • Journal Title: Mol Hum Reprod 33 Pages: 188-99
  • Peer Reviewed
• [Journal Article] Temporal and spatial expression of tumor-associated antigen RCAS1 in pregnant mouse uterus (2010)
  • Author(s): Tskitishvili E, Nakamura H, Kinugasa-Taniguchi Y, Kanagawa T, Kimura T, Tomimatsu T, Shimoya K.
  • Journal Title: Am J Reprod Immunol. 63 Pages: 137-43
  • Peer Reviewed
• [Journal Article] Nicotine restores endothelial dysfunction caused by excess sFlt1 and sEng in in vitro model of preeclamptic vascular endothelium : a possible therapeutic role of nicotinic acetylcholine receptor (nAChR) agoonists for preeclampsia. (2010)
  • Author(s): Mimura K, Kanagawa T
  • Journal Title: Am J Obstet Gynecol Volume: 202
  • Peer Reviewed
• [Journal Article] Human C-reactive protein enhances vulnerability of immature rats to hypoxic-ischemic brain damage ; a preliminary study. (2010)
  • Author(s): Kinugasa-Taniguchi Y, Kanagawa T
  • Journal Title: Reprod Sci Volume: 17 Pages: 419-425
  • Peer Reviewed
• [Journal Article] Nicotine suppresses interleukin-6 production from vascular endothelial cells : a possible therapeutic role of nicotine for preeclampsia. (2010)
  • Author(s): Sharentuya N, Kanagawa T
  • Journal Title: Reprod Sci Volume: 17 Pages: 556-563
  • Peer Reviewed
• [Journal Article] A retrospective analysis of ovarian endometriosis during pregnancy. (2010)
  • Author(s): Ueda Y, Kanagawa T
  • Journal Title: Fertil Steril Volume: 94 Pages: 78-84
  • Peer Reviewed
• [Journal Article] 出生前に消極的治療方針が選択された新生児外科症例の検討 (2010)
  • Author(s): 臼井規朗、金川武司
  • Journal Title: 日本周産期・新生児医学会誌 Volume: 46 Pages: 32-38
  • NAID: 10026414098
  • Peer Reviewed
• [Journal Article] Maternal blood serum and plasma human tumor- associated antigen RCAS1 during the course of uncomplicated pregnancies : a prospective study. (2010)
  • Author(s): Tskitishvili E, Kanagawa T
  • Journal Title: Am J Reprod Immunol Volume: 64 Pages: 218-224
  • Peer Reviewed
• [Journal Article] Impaired delivery outcomes in pregnancies following myomectomy compared to myoma-complicated pregnancies. (2010)
  • Author(s): Kinugasa-Taniguchi Y, Kanagawa T
  • Journal Title: J Reprod Med Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Current status of negative treatment decision-making for fetuses with a prenatal diagnosis of neonatal surgical disease at a single Japanese institution. (2010)
  • Author(s): Usui N, Kanagawa T
  • Journal Title: J Pediatr Surg Volume: 46 Pages: 229-234
  • Peer Reviewed
• [Journal Article] Human C-reactive protein enhances vulnerability of immature rats to hypoxic-ischemic brain damage ; a preliminary study (2010)
  • Author(s): Kinugasa-Taniguchi Y., et al.
  • Journal Title: Reprod Sci
  • Peer Reviewed
• [Journal Article] Nicotine suppresses interleukin-6 production from vascular endothelial cells : a possible therapeutic role of nicotine for preeclampsia (2010)
  • Author(s): Sharentuya N., et al.
  • Journal Title: Reprod Sci
  • Peer Reviewed
• [Journal Article] 本学におけるActive Management of Labor. (2009)
  • Author(s): 荻田和秀, 冨松拓治, 澤田健二郎, 上田豊, 金川武司, 衣笠友基子, 坂田正博, 木村正
  • Journal Title: 産婦人科の進歩 61 Pages: 101-3
  • NAID: 130000137673
  • Peer Reviewed
• [Journal Article] Amniotic fluid 'sludge' detected in patients with subchorionic hematoma:a report of two cases (2009)
  • Author(s): Tskitishvili E, Tomimatsu T, Kanagawa T, Sawada K, Kinugasa Y, Mimura K, Kimura T.
  • Journal Title: Ultrasound Obstet Gynecol 33 Pages: 484-6
  • Peer Reviewed
• [Journal Article] Cesarean scar pregnancies successfully treated with methotrexate (2009)
  • Author(s): Muraji M, Mabuchi S, Hisamoto K, Muranishi M, Kanagawa T, Nishio Y, Kimura T.
  • Journal Title: Acta Obstet Gynecol Scand 88 Pages: 720-3
  • Peer Reviewed
• [Journal Article] A case of recurrent cesareanscarpregnancy. (2009)
  • Author(s): Mabuchi S, Kawase C, Muraji M, Kanagawa T, Kimura T
  • Journal Title: Acta obstet Gynecol Scand 88 Pages: 1059-60
  • Peer Reviewed
• [Journal Article] Postpartum outcome of cervical intraepithelial neoplasia in pregnant women determined by route of delivery. (2009)
  • Author(s): Ueda Y, Enomoto T, Miyatake T, Yoshino K, Fujita M, Miyake T, Fujiwara K, Muraji M, Kanagawa T, Kimura T
  • Journal Title: Reprod Sci 16 Pages: 1034-9
  • Peer Reviewed
• [Journal Article] 【出生前診断の倫理と実際】出生前診断された腹壁異常治療の実際と倫理的問題点 (2009)
  • Author(s): 臼井規朗, 金川武司
  • Journal Title: 小児外科 41 Pages: 494-9
  • Peer Reviewed
• [Journal Article] ●多胎妊娠、子宮外妊娠、繊毛性疾患●母体搬送、産褥搬送●輸血・輸液●その他の偶発合併症 (2009)
  • Author(s): 金川武司
  • Journal Title: ビジュアル産科
  • Peer Reviewed
• [Journal Article] Nicotine restores endothelial dysfunction caused by excess sFlt1 and sEng in in vitro model of preeclamptic vascular endothelium : apossible therapeutic role of nicotinic acetylcholine receptor (nAChR) agoonists for preeclampsia (2009)
  • Author(s): Mimura K., et al.
  • Journal Title: Am J Obstet Gynecol
  • Peer Reviewed
• [Journal Article] Spatio-Temporal Image Correlation(STIC)used by generalobstetriclan : is as effective as 2D fetal echocardiography scanned by experts (2008)
  • Author(s): Kanagava T, Wanitpongpan p, Kinugasa Y, Kinura T
  • Journal Title: Prenat Di agn 28(10) Pages: 923-8
  • Peer Reviewed
• [Journal Article] The diagnostic utility of the fetal nasal bone length in the short-limbed fetus 、 (2008)
  • Author(s): Kanagawa T, Tomlmatsu T, Klnugasa Y, Mimura K, Kimura T
  • Journal Title: Congenital Anomalies 48(2)
  • Peer Reviewed
• [Presentation] 出生前診断 (2010)
  • Author(s): 金川武司
  • Organizer: 第49回大阪母性衛生学会
  • Place of Presentation: 大阪大学中之島センター,大阪
  • Year and Date: 2010-12-19
• [Presentation] 出生前診断(座長) (2010)
  • Author(s): 金川武司
  • Organizer: 第49回 大阪母性衛生学会
  • Place of Presentation: 大阪大学中之島センター(招待講演)
  • Year and Date: 2010-12-19
• [Presentation] 出生前診断 (2010)
  • Author(s): 金川武司
  • Organizer: 第41回阪神周産期勉強会
  • Place of Presentation: 西宮市民会館,兵庫
  • Year and Date: 2010-11-25
• [Presentation] 出生前診断 (2010)
  • Author(s): 金川武司
  • Organizer: 第41回 阪神周産期勉強会
  • Place of Presentation: 西宮市民会館(招待講演)
  • Year and Date: 2010-11-25
• [Presentation] Nicotine restores endothelial dysfunction caused by excess sFlt1 and sEng in in vitro model of preeclamptic vascular endothelialium (2009)
  • Author(s): 味村和哉, et al.
  • Organizer: 第3回 世界産婦人科研究機構サミット
  • Place of Presentation: 仙台国際センター (宮城県)
  • Year and Date: 2009-11-12
• [Presentation] Nicotine suppresses interleukin-6 production from vascular endothelial cells (2009)
  • Author(s): Tomimatsu T., et al.
  • Organizer: 第3回 世界産婦人科研究機構サミット
  • Place of Presentation: 仙台国際センター (宮城県)
  • Year and Date: 2009-11-12
• [Presentation] β-ラクタム抗生剤でのプレコンディショニングによる新生児脳障害予防の可能性 (2009)
  • Author(s): 味村和哉, 他
  • Organizer: 第45回 日本周産期・新生児医学会
  • Place of Presentation: 名古屋国際会館 (愛知県)
  • Year and Date: 2009-07-14
• [Presentation] ニコチンは妊娠高血圧症候群の治療薬となりうるか? -基礎的検討- (2009)
  • Author(s): 沙仁吐雅, 他
  • Organizer: 第61回日本産科婦人科学会
  • Place of Presentation: 国立京都国際会館 (京都府)
  • Year and Date: 2009-04-05
• [Presentation] Nicotine restores endothelial dysfunction caused by excess sFlt1 and sEng in in vitro model of preeclamptic vascular endothelialium. (2009)
  • Author(s): Mimura K, Tomimatsu T, Sharentuya N, Tskitishvili E, Kinugasa-Taniguchi Y, Kanagawa T, Kimura T
  • Organizer: 第3回世界産婦人科研究機構サミット
  • Place of Presentation: 仙台国際センター,宮城
• [Presentation] Nicotine suppresses interleukin-6 production from vascular endothelial cells. (2009)
  • Author(s): Tomimatsu T, Syarentuya N, Mimura K, Tskitishvili E, Kinugasa-Taniguchi Y, Kanagawa T, Kimura T
  • Organizer: 第3回世界産婦人科研究機構サミット
  • Place of Presentation: 仙台国際センター,宮城
• [Presentation] Possible connection between sEndoglin and S100B protein might lead to the development of endothelial disfunction and preeclampsia. (2009)
  • Author(s): Tskitishvili E, Sharentuya N, Temma-Asano K, Tomimatsu T, Kinugasa-Taniguchi Y, Kanagawa T, Kimura T, Shimoya K.
  • Organizer: 第3回世界産婦人科研究機構サミット
  • Place of Presentation: 仙台国際センター,宮城
• [Presentation] 分娩前まで持続したCINは帝王切開より経膣分娩の方が有意に退縮しやすい (2009)
  • Author(s): 金雅子, 藤田征巳, 松崎慎哉, 金川武司, 吉野潔, 村田雄二
  • Organizer: 第61回日本産科婦人科学会
  • Place of Presentation: 国立京都国際会館,京都
• [Presentation] 妊娠に合併した卵巣子宮内膜症性嚢胞の検討 (2009)
  • Author(s): 上田豊, 榎本隆之, 宮武崇, 連美穂, 藤田征巳, 金川武司, 山本亮, 山本敏也, 清水廣, 吉野潔, 村田雄二, 木村正
  • Organizer: 第61回日本産科婦人科学会
  • Place of Presentation: 国立京都国際会館,京都
• [Presentation] 口唇・口蓋裂の胎児診断 (2009)
  • Author(s): 金川武司
  • Organizer: 第33回遺伝カウンセリングリフレッシュセミナー
  • Place of Presentation: 大阪
• [Presentation] 当院における出生前診断の適応と帰結 (2008)
  • Author(s): 衣笠友基子, 味村和哉, 香山晋輔, 上田豊, 金川武司, 澤田健二郎, 冨松拓治, 木村正
  • Organizer: 第119回近畿産婦人科学会
  • Place of Presentation: 千里ライフサイエンスセンター,大阪
  • Year and Date: 2008-11-09
• [Presentation] 前置胎盤合併に対して、子宮内胎児死亡誘導後に中期中絶術を施行した2症例 (2008)
  • Author(s): 金川武司, 味村和哉, 香山晋輔, 衣笠友基子, 上田豊, 澤田健二郎, 冨松拓治, 坂田正博, 木村正
  • Organizer: 第119回近畿産婦人科学会
  • Place of Presentation: 千里ライフサイエンスセンター,大阪
  • Year and Date: 2008-11-09
• [Presentation] 妊娠中に腫大した卵巣子宮内膜症(chocolate cyst) (2008)
  • Author(s): 連美穂, 上田豊, 榎本隆之, 宮武崇, 藤田征巳, 山本亮, 金川武司, 木村正
  • Organizer: 第119回近畿産婦人科学会
  • Place of Presentation: 千里ライフサイエンスセンター,大阪
  • Year and Date: 2008-11-09
• [Presentation] 産科超音波の基礎-妊娠初期から中期までの超音波スクリーニング- (2008)
  • Author(s): 金川武司
  • Organizer: 大阪大学産科婦人科専攻医クルズス
  • Place of Presentation: 大阪大学,大阪
  • Year and Date: 2008-10-30
• [Presentation] 妊娠初期から中期までの超音波スクリーニングと精査 (2008)
  • Author(s): 金川武司
  • Organizer: 第1回大阪産婦人科臨床フォーラム
  • Place of Presentation: 大阪
  • Year and Date: 2008-08-09
• [Presentation] 出生前超音波検査で認めた四肢短縮例における鼻骨長計測の有用性 (2008)
  • Author(s): 金川武司, 冨松拓治, 衣笠友基子, 味村和哉, 木村正
  • Organizer: 第48回日本先天異常学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-29
• [Presentation] 出生前超音波検査で認めた四肢短縮例における鼻骨長計測の有用性 (2008)
  • Author(s): 金川武司 冨松拓治 衣笠友基子 味村和哉 木村正
  • Organizer: 第48回日本先天異常学会学術集会
  • Place of Presentation: 聖路加病院東京
  • Year and Date: 2008-06-29
• [Presentation] 湿布の大量貼付によって引き起こされたと思われる胎児動脈管早期収縮の一例 (2008)
  • Author(s): 金川武司, 冨松拓治, 衣笠友基子, 荻田和秀, 荒堀仁美, 小垣滋豊, 和田和子, 木村正
  • Organizer: 第10回大阪新生児研究会
  • Place of Presentation: 大阪薬業年金会館,大阪
  • Year and Date: 2008-06-07
• [Presentation] ニコチンの妊娠高血圧症候群治療薬としての可能性～HUVECと抗血管新生因子を用いた妊娠高血圧症候群モデルを用いて～. (2008)
  • Author(s): 味村和哉, 冨松拓治, 沙仁吐雅, 衣笠友基子, 金川武司, 木村正
  • Organizer: 第29回日本妊娠高血圧学会
  • Place of Presentation: 福島ビューホテル,福島
• [Presentation] 湿布の大量貼付によって引き起こされたと思われる胎児動脈管早期収縮の一例 (2008)
  • Author(s): 金川武司, 冨松拓治, 衣笠友基子, 荻田和秀, 荒堀仁美, 小垣滋豊, 和田和子, 木村正
  • Organizer: 第44回日本周産期・新生児医学会
  • Place of Presentation: パシフィコ横浜, 神奈川
• [Presentation] 横隔膜ヘルニアの治療ストラテジー予測重症度に基づいた胎児横隔膜ヘルニアの治療方針 (2008)
  • Author(s): 臼井規朗, 奥山宏臣, 澤井利夫, 福澤正洋, 窪田昭男, 金川武司, 木村正, 稲村昇, 濱中拓郎, 末原則幸
  • Organizer: 第44回日本周産期・新生児医学会
  • Place of Presentation: パシフィコ横浜,神奈川